Platinum sulfide protein nanoparticle having near-infrared photothermal effect and multimodal imaging function, preparation method therefor and application thereof

Patent metadata

Field	Value
Publication number	US-11364312-B2
Application number	US-202117169455-A
Country	US
Kind code	B2
Filing date	Feb 6, 2021
Priority date	Aug 7, 2018
Publication date	Jun 21, 2022
Grant date	Jun 21, 2022

Abstract

A platinum sulfide protein nanoparticle having near-infrared photothermal effect and multi-modal imaging function, a preparation method therefor and an application thereof. The platinum sulfide nanoparticle having near-infrared photothermal effect and multi-modal imaging function is prepared in aqueous phase by means of formulation screening and process limitation. The nanoparticle has an ultra-small particle size and good stability as well as tumor targeting and photothermal effects and integrates functions of near-infrared imaging, CT imaging, and thermal imaging, so as to achieve high sensitivity, high resolution, and precise positioning of tumors, and to produce high-efficiency photothermal effects under the excitation of near-infrared light to kill tumor cells by thermal ablation, thereby achieving the purpose of efficient, safe, visual, and accurate treatment of tumors. The nanoparticle has the potential for further development and clinical application.

First claim

The invention claimed is:

1. A method of preparing a platinum sulfide protein nanoparticle with near-infrared photothermal effect and multi-modal imaging function, consisting of the following steps:
mixing a platinum dichloride solution with a protein solution,
adding sodium sulphide solution,
reacting to form a mixture, and
dialyzing and ultrafiltrating the mixture to obtain the platinum sulfide protein nanoparticle with near-infrared photothermal effect and multi-modal imaging function,
wherein a concentration of the platinum dichloride solution is 2 to 8 mmol/L; a concentration of the protein solution is 1 to 9 mg/mL; a concentration of the sodium sulphide solution is 1-50 mmol/L; a volume ratio of the platinum dichloride solution, the protein solution, and the sodium sulfide solution is 1:0.2:0.05;
wherein a reaction temperature is 0 to 55° C., a reaction time is 0 to 5 h;
wherein a cut-off molecular weight for dialyzing is 3500 kD, dialyzing dialysis time is 1 to 24 h, a receiving medium for dialyzing is deionized water, and the receiving medium is changed 6 to 8 times; and
wherein a cut-off molecular weight for ultrafiltrating is 100 kD, a rotation speed of ultrafiltrating is 1500 to 4000 r/min, and a number of ultrafiltrating is at least 20 times.

2. A method of preparing a reagent with near-infrared photothermal effect and multi-modal imaging function, consisting of the following steps:
mixing a platinum dichloride solution with a protein solution,
adding a sodium sulphide solution,
reacting to form a mixture; and
dialyzing and ultrafiltrating the mixture to obtain a platinum sulfide protein nanoparticle with near-infrared photothermal effect and multi-modal imaging function, and
dispersing the platinum sulfide protein nanoparticle with deionized water to obtain the reagent with near-infrared photothermal effect and multi-modal imaging function,
wherein a concentration of the platinum dichloride solution is 2 to 8 mmol/L; a concentration of the protein solution is 1 to 9 mg/mL; a concentration of the sodium sulphide solution is 1-50 mmol/L; a volume ratio of the platinum dichloride solution, the protein solution, and the sodium sulfide solution is 1:0.2:0.05;
wherein a reaction temperature is 0 to 55° C., a reaction time is 0 to 5 h;
wherein a cut-off molecular weight for dialyzing is 3500 kD, dialyzing dialysis time is 1 to 24 h, a receiving medium for dialyzing is deionized water, and the receiving medium is changed 6 to 8 times; and
wherein a cut-off molecular weight for ultrafiltrating is 100 kD, a rotation speed of ultrafiltrating is 1500 to 4000 r/min, and a number of ultrafiltrating is at least 20 times.

3. The method according to claim 1 , wherein a diameter of the platinum sulfide protein nanoparticle with near-infrared photothermal effect and multi-modal imaging function is 1 to 5 nm.

4. The method according to claim 3 , wherein the platinum sulfide protein nanoparticle with near-infrared photothermal effect and multi-modal imaging function consists of a platinum sulfide protein nanoparticle scaffold and a the platinum sulfide core.

Assignees

• Univ Soochow

Inventors

• Yang Hong
• Chen Huabing
• Wang Xue
• Ke Hengte
• Li Ming
• Xu Tao
• Zhang Miya

Classifications

• A61K9/5169

  Proteins, e.g. albumin, gelatin · CPC title

• A61K9/5192

  Processes · CPC title

• A61K9/5115

  Inorganic compounds · CPC title

• A61K41/0052 Primary

  Thermotherapy; Hyperthermia; Magnetic induction; Induction heating therapy · CPC title

• A61K49/04

  X-ray contrast preparations · CPC title