New process and intermediates
US-2018354889-A1 · Dec 13, 2018 · US
Engineered transaminase polypeptides for industrial biocatalysis
US11359183B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11359183-B2 |
| Application number | US-201816615999-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 23, 2018 |
| Priority date | Jun 14, 2017 |
| Publication date | Jun 14, 2022 |
| Grant date | Jun 14, 2022 |
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- Title
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Abstract
Official abstract text for this publication.
The present invention provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The invention also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.
First claim
Opening claim text (preview).
We claim: 1. An engineered transaminase comprising a polypeptide sequence having at least 97% sequence identity to SEQ ID NO:4, wherein said engineered transaminase comprises amino acid substitutions in said polypeptide sequence at positions 85 and 422, wherein the amino acid substitution at position 422 is V422C, V422G, or V422A, and the amino acid substitution at position 85 is F85V, wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:4, and wherein said engineered transaminase exhibits greater enzymatic activity and greater stereoselectivity toward the (S) amine diastereomer than the transaminase of SEQ ID NO:4. 2. The engineered transaminase of claim 1 , wherein said engineered transaminase comprises a polypeptide sequence that has at least 97% sequence identity to the sequence of the transaminase variant set forth in SEQ ID NO: 30. 3. The engineered transaminase of claim 1 , wherein said engineered transaminase comprises a polypeptide sequence of SEQ ID NO: 30. 4. The engineered transaminase of claim 1 , wherein said engineered transaminase comprises at least one improved property compared to wild-type V. fluvialis transaminase. 5. The engineered transaminase of claim 4 , wherein said improved property comprises improved enzymatic activity on a substrate having the following structure: 6. The engineered transaminase of claim 4 , wherein said improved property comprises improved thermostability. 7. The engineered transaminase of claim 1 , wherein said engineered transaminase is purified. 8. A composition comprising the engineered transaminase of claim 1 .
Assignees
Inventors
Classifications
- C12N9/1096Primary
transferring nitrogenous groups (2.6) · CPC title
Transaminases (2.6.1) · CPC title
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression · CPC title
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- What does patent US11359183B2 cover?
- The present invention provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The invention also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral a…
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/1096. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 14 2022 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 7 related publications on this page (citations in our corpus or others sharing the same primary CPC).