Method for efficient production of psicose

Patent metadata

Field	Value
Publication number	US-11358980-B2
Application number	US-201716463977-A
Country	US
Kind code	B2
Filing date	Nov 24, 2017
Priority date	Dec 8, 2016
Publication date	Jun 14, 2022
Grant date	Jun 14, 2022

Abstract

The present invention relates to a method for effectively utilizing fructose raffinate obtained in the process for separating psicose conversion product with a high purity chromatography in the process for preparing psicose, and more specifically, it is utilized for preparation of fructose-containing raw material solution for preparation of psicose by putting fructose raffinate obtained in the process for preparing psicose into the process for preparing fructose.

First claim

The invention claimed is:

1. A method of preparation for psicose comprising:
separating psicose-conversion product with a simulated moving bed (SMB) chromatography to obtain a psicose fraction and a fructose raffinate,
providing the fructose raffinate into a separation process of fructose preparation to prepare fructose-containing raw material, and
conducting psicose conversion reaction by using the fructose-containing raw material,
wherein the psicose conversion product are obtained by a biological psicose conversion process on fructose raw material using a biocatalyst having a conversion activity from fructose to psicose, and
wherein the separation process of fructose preparation comprises an ion purification step which removes ion from fructose isomerization product, and a separation step using a SMB chromatography, and the fructose raffinate is provided to one or more kinds of steps selected from the group consisting of the ion purification step and the separation step using SMB chromatography.

2. The method of preparation of claim 1 , wherein the separation process of fructose preparation comprises a primary ion purification step which removes ion from fructose isomerization product, a separation step using a SMB chromatography, a secondary ion purification step and a concentration step, and the fructose raffinate is provided to one or more kinds of steps selected from the group consisting of the primary ion purification step, the high purity separation step using the SMB chromatography and the secondary ion purification step.

3. The method of preparation of claim 1 , wherein the fructose-containing raw material has an electrical conductivity of 0 to 15 μs/cm.

4. The method of preparation of claim 1 , wherein the fructose-containing raw material has a calcium ion concentration of 0.05 mM or lower.

5. The method of preparation of claim 1 , wherein the fructose raffinate obtained in the separating step with using the SMB chromatography has an electrical conductivity of 20 to 200 μs/cm.

6. The method of preparation of claim 1 , wherein 65 to 95% by weight of a fructose solution obtained in the fructose preparation is mixed with 5 to 35% by weight of the fructose raffinate based on the total 100% by weight of the fructose solution and the fructose raffinate, when the fructose solution and the fructose raffinate are adjusted to 50 Brix each.

7. The method of preparation of claim 1 , wherein the fructose content of fructose-containing raw material which is put into the psicose conversion reaction is 85% by weight or higher based on 100% by weight of the total saccharides content.

8. The method of preparation of claim 1 , wherein the fructose raffinate obtained in the separating step with the SMB chromatography comprises 85 to 99% by weight of fructose and 2.0% by weight or lower of psicose based on 100% by weight of the total saccharides.

9. The method of preparation of claim 1 , wherein the separating step with SMB chromatography process is carried out with a column chromatograph packed with cation exchange resin in which a calcium active group is attached.

10. The method of preparation of claim 1 , wherein the psicose conversion reaction uses a biological catalyst having a psicose coversion ratio of 15% to 70%.

11. The method of preparation of claim 1 , further comprising a step of cooling the fructose raffinate obtained in the separating step with the SMB chromatography by a heat exchanger.

12. The method of preparation of claim 1 , further comprising a step of concentrating the psicose fraction and crystallizing psicose from the concentrates to obtain psicose crystal and crystallization mother liquor.

Assignees

• Samyang Corp

Inventors

• Park Ji Won
• Park Sung Won
• Park Chong Jin

Classifications

• C07H3/02 Primary

  Monosaccharides · CPC title

• B01D15/185 Primary

  characterised by the components to be separated · CPC title

• B01D15/361

  Ion-exchange · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C07H1/06

  Separation; Purification · CPC title