Agarose ultrafiltration membrane composites for size based separations

Patent metadata

Field	Value
Publication number	US-11358102-B2
Application number	US-202016918431-A
Country	US
Kind code	B2
Filing date	Jul 1, 2020
Priority date	Aug 17, 2015
Publication date	Jun 14, 2022
Grant date	Jun 14, 2022

Abstract

The embodiments described herein relate to agarose ultrafiltration membrane composites and methods for making and using the same.

First claim

What is claimed is:

1. A method for making an agarose ultrafiltration membrane composite, the method comprising the steps of:
i. providing a porous support membrane having an average pore size ranging from 0.01 μm to 10 μm and an average thickness ranging from 20 μm to 150 μm,
ii. providing an agarose solution, wherein the agarose solution further comprises ZnCl2 at a concentration equal to or less than 15 wt.-%, and
iii. casting a layer of the agarose solution onto the porous support membrane, and
iv. immersing the agarose coated porous support membrane into a water bath at a temperature below the gelling point of the agarose solution
in an agarose ultrafiltration membrane composite having average pore size less than 0.1 μm.

2. The method according to claim 1 , wherein the porous support membrane comprises a polymer selected from the group consisting of polyethylene, polypropylene, polycaprolactam, poly(hexamethylene adipamide), polyethylene terephthalate, polyether-ether ketone, polysulfone, polyethersulfone, polytetrafluoroethylene, and polyvinylidene fluoridepolyvinylidene fluoride (PVDF).

3. The method according to claim 1 , wherein the porous support membrane comprises a polymer selected from the group consisting of polyvinylidene fluoride (PVDF), ultrahigh molecular weight polyethylene (UHMW-PE), polycaprolactam, poly(hexamethylene adipamide), polysulfone, and polyethersulfone.

4. The method according to claim 1 , wherein the agarose solution in (ii) comprises agarose at a concentration ranging from 1-12 wt.-%.

5. The method of claim 2 , wherein the agarose solution in (ii) comprises a crosslinker at a concentration ranging from 0.01 wt.-% to 1 wt.-%.

6. The method according to claim 1 , wherein the agarose solution in (ii) comprises ZnCl2 at a concentration equal to or less than 15 wt.-% and divinylsulfone (DVS) as crosslinker.

7. The method according to claim 1 , wherein agarose solution in step (ii) is heated to a temperature ranging from 20 to 90° C.

8. The method according to claim 1 , wherein the porous support membrane in step (iii) is heated to a temperature ranging from 20 to 90° C.

9. The method according to claim 1 , wherein in step iv) the agarose coated porous support membrane is immersed in a water bath at a temperature ranging from 5-60° C.

10. The method according to claim 1 , wherein in step i) a porous support membrane is provided having an average thickness between 100 μm-200 μm and an average pore size of 0.2 μm.

11. A method for making an agarose ultrafiltration membrane composite, comprising:
i. providing a porous support membrane having an average pore size ranging from 0.01 μm to 10 μm and an average thickness ranging from 20 μm to 150 μm,
ii. providing an agarose solution, wherein the agarose solution comprises a crosslinker at a concentration ranging from 0.01 wt.-% to 1 wt.-%,
iii. casting a layer of the agarose solution onto the porous support membrane, wherein the agarose layer infiltrates the porous support membrane,
iv. immersing the agarose coated porous support membrane into a water bath at a temperature below the gelling point of the agarose solution, wherein the agarose layer forms a hydrogel, and
v. cooling the agarose coated porous support membrane,
wherein an agarose ultrafiltration membrane composite having average pore size less than 0.1 μm is solidified, making an agarose ultrafiltration membrane composite.

Assignees

• Emd Millipore Corp

Inventors

• Jaber Jad
• Moya Wilson
• Kozlov Mikhail

Classifications

• B01D67/0013

  Casting processes · CPC title

• B01D67/00113

  Pretreatment of the casting solutions, e.g. thermal treatment or ageing · CPC title

• B01D2325/0283

  Pore size · CPC title

• B01D69/107

  Organic support material · CPC title

• B01D67/0016

  Coagulation · CPC title