Methods of making gels and films using curli nanofibers
US-11098133-B2 · Aug 24, 2021 · US
Genetic reprogramming of bacterial biofilms
US11352397B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11352397-B2 |
| Application number | US-201916713368-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 13, 2019 |
| Priority date | Apr 23, 2013 |
| Publication date | Jun 7, 2022 |
| Grant date | Jun 7, 2022 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
Described herein are methods and compositions relating to engineered curli fibers, e.g. CsgA polypeptide. In some embodiments, the methods and compositions described herein relate to functionalized biofilms.
First claim
Opening claim text (preview).
What is claimed herein is: 1. An engineered CsgA polypeptide comprising a wild-type Escherichia coli ( E. coli ) CsgA polypeptide with a C-terminal display tag flanking the CsgA, wherein the display tag comprises an activity polypeptide and a flexible polypeptide linker sequence, wherein the linker sequence is located N-terminal to the display tag, wherein the activity polypeptide comprises a binding domain selected from the group consisting of SpyTag peptide, metal binding domain (MBD) peptide, graphene binding peptide (GBP), carbon nanotube binding peptide (CBP), gold binding A3 peptide, ZnS quantum dot templating CT43 peptide, FLAG affinity tag peptide, ZnS quantum dot templating Z8 peptide, CdS quantum dot templating E14 peptide, quartz/glass binding QBP1 peptide, hydroxyapatite templating CLP12 peptide, ice crystal binding AFP8 peptide, biotin acceptor peptide (BAP), biotin carboxyl carrier protein (BCCP), and a peptide comprising a LPXTG (SEQ ID NO: 23) motif. 2. The CsgA polypeptide of claim 1 , wherein the linker sequence consists of glycine and serine residues. 3. A composition comprising the engineered CsgA polypeptide of claim 1 . 4. The composition of claim 3 , wherein the composition comprises filaments comprising the engineered CsgA polypeptide of claim 1 . 5. The composition of claim 3 , wherein the composition comprises a proteinaceous network. 6. The composition of claim 3 , wherein the composition further comprises proteinaceous biofilm components. 7. The composition of claim 3 , wherein the composition further comprises an engineered microbial cell comprising a vector or nucleic acid sequence encoding the engineered CsgA polypeptide. 8. The composition of claim 3 , wherein the composition further comprises a functionalizing polypeptide comprising an interacting domain.
Assignees
Inventors
Classifications
Antibacterial agents · CPC title
- C07K14/245Primary
Escherichia (G) · CPC title
containing a domain for self-assembly, e.g. a viral coat protein (includes phage display) · CPC title
containing a tag with affinity for a non-protein ligand · CPC title
from Pseudomonadaceae (F) · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US11352397B2 cover?
- Described herein are methods and compositions relating to engineered curli fibers, e.g. CsgA polypeptide. In some embodiments, the methods and compositions described herein relate to functionalized biofilms.
- Who is the assignee on this patent?
- Harvard College
- What technology area does this patent fall under?
- Primary CPC classification C07K14/245. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 07 2022 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 7 related publications on this page (citations in our corpus or others sharing the same primary CPC).