Solid medium for producing glucosamine and its application

Patent metadata

Field	Value
Publication number	US-11345942-B2
Application number	US-201916562529-A
Country	US
Kind code	B2
Filing date	Sep 6, 2019
Priority date	Sep 17, 2018
Publication date	May 31, 2022
Grant date	May 31, 2022

Abstract

The invention relates to solid medium for producing glucosamine, including a substrate and 0.15-4.8 mL/g substrate of supplemental solution, which includes 0.1-2 g/L KH 2 PO 4 , 0.1-2 g/L NaCl, and 0.1-2 g/L MgSO 4 .7H 2 O. The invention also relates to a method for producing glucosamine, including providing microorganism being able to produce glucosamine, and fermenting the microorganism in the medium mentioned above.

First claim

What is claimed is:

1. A method for producing glucosamine, comprising:
providing a microorganism being able to produce glucosamine, wherein the microorganism is Aspergillus sydowii ; and
fermenting the microorganism in a solid medium which comprises substrate and 0.15-4.8 mL/g substrate of supplemental solution;
wherein the substrate is wheat bran without alkaline pretreatment and the supplemental solution including
ental solution including
0.1-2 g/L KH 2 PO 4 ;
0.1-2 g/L NaCl; and
0.1-2 g/L MgSO 4 .7H 2 O.

2. The method of claim 1 , wherein the substrate is treated by high temperature, high pressure, or the combination thereof.

3. The method of claim 1 , wherein the substrate is in a container and has a thickness of 0.5-2.5 mm.

4. The method of claim 1 , wherein the microorganism has an inoculum amount of 9 to 36 mg/g substrate.

5. The method of claim 1 , wherein the solid medium further comprises 5-50 percentage by volume of molasses, and the molasses is pretreated with the following steps: mixing a molasses stock with water at a ratio of 0.5:1 to 5:1 by volume, allowing the mixed molasses stock and water to settle and form an upper layer and a lower layer, and collecting the upper layer as treated molasses.

6. The method of claim 5 , wherein the supplemental solution is divided into a first portion and a second portion, the first portion has 50-60 percentage by volume (vol %) of the supplemental solution, and the second portion has 40-50 vol % of the supplemental solution, and the first portion is added to the substrate before the second portion is added to the substrate.

7. The method of claim 1 , wherein the microorganism is fermented for 48-120 hours.

8. The method of claim 1 , wherein glucosamine is harvested when the microorganism begins to produce spore capsule.

Assignees

• Univ Yuan Ze

Inventors

• Wu Ho-Shing
• Peng jia-wei

Classifications

• C12N1/14

  Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor · CPC title

• C12P19/26 Primary

  Preparation of nitrogen-containing carbohydrates · CPC title

• C12N2500/30

  Organic components · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12N2500/16

  Magnesium; Mg chelators · CPC title