Genetically modified bacillus subtilis strain and use as a live delivery and production system
US-2024390433-A1 · Nov 28, 2024 · US
Production of frambinone by a recombinant fungal microorganism
US11345936B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11345936-B2 |
| Application number | US-202017004863-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 27, 2020 |
| Priority date | Jun 3, 2016 |
| Publication date | May 31, 2022 |
| Grant date | May 31, 2022 |
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- Title
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Abstract
Official abstract text for this publication.
The invention relates to a genetically modified fungal microorganism for the production of frambinone, the microorganism having the following characteristics: —the capacity to produce frambinone from tyrosine; and —a limited capacity or no capacity to break tyrosine down into tyrosol, p-hydroxyphenylacetaldehyde and/or p-hydroxyphenylacetate; and to the use of same for producing frambinone.
First claim
Opening claim text (preview).
The invention claimed is: 1. A process for the production of frambinone comprising culturing a genetically modified fungal microorganism with a capacity to produce frambinone from tyrosine in a medium comprising tyrosine and a repressor of the path for breaking tyrosine down into tyrosol, p-hydroxyphenylacetaldehyde and/or p-hydroxyphenylacetate, and wherein the repressor is glutamate. 2. The process for the production of frambinone according to claim 1 characterized in that the microorganism (a) belongs to the phyla chosen from among the ascomycetes or basidiomycetes, (b) includes at least one heterologous sequence encoding the enzyme 4-coumarate: CoA ligase (4CL) or benzalacetone synthase (BAS), (c) comprises at least one heterologous or supernumerary sequence encoding the enzyme tyrosine ammonia lyase (TAL) or benzalacetone reductase (BAR), (d) comprises at least one heterologous or supernumerary sequence encoding the enzyme phenylalanine ammonia lyase (PAL) or cinnamate 4 hydroxylase (C4H), or (e) comprises any combination of characteristics (a)-(d). 3. The process for the production of frambinone according to claim 1 characterized in that the microorganism is the industrial strain Saccharomyces cerevisiae RK4 registered with the CNCM on Jun. 1, 2016 under number I-5101. 4. The process for the production of frambinone according to claim 1 characterized in that the microorganism is the industrial strain Saccharomyces cerevisiae RK5 registered with the CNCM on Apr. 26, 2017 under number I-5199. 5. The process for the production of frambinone according to claim 1 characterized in that the microorganism belongs to the genus Yarrowia, Debaryomyces, Arxula, Scheffersomyces, Geotrichum, Pichia , or Saccharomyces. 6. The process for the production of frambinone of claim 2 characterized in that the microorganism includes at least one heterologous sequence encoding 4CL and BAS, at least one heterologous or supernumerary sequence encoding TAL and BAR, and/or at least one heterologous or supernumerary sequence encoding PAL or C4H.
Assignees
Inventors
Classifications
aromatic · CPC title
acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title
- C12N15/52Primary
Genes encoding for enzymes or proenzymes · CPC title
Saccharomyces isolates · CPC title
transferring groups other than amino-acyl groups (2.3.1) · CPC title
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Frequently asked questions
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- What does patent US11345936B2 cover?
- The invention relates to a genetically modified fungal microorganism for the production of frambinone, the microorganism having the following characteristics: —the capacity to produce frambinone from tyrosine; and —a limited capacity or no capacity to break tyrosine down into tyrosol, p-hydroxyphenylacetaldehyde and/or p-hydroxyphenylacetate; and to the use of same for producing frambinone.
- Who is the assignee on this patent?
- Lesaffre & Cie
- What technology area does this patent fall under?
- Primary CPC classification C12N15/52. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue May 31 2022 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).