Engineered enzymes

Patent metadata

Field	Value
Publication number	US-11345903-B2
Application number	US-202016953253-A
Country	US
Kind code	B2
Filing date	Nov 19, 2020
Priority date	Oct 22, 2018
Publication date	May 31, 2022
Grant date	May 31, 2022

Abstract

The present disclosure provides engineered RNA-guided enzymes for editing live cells.

First claim

We claim:

1. An engineered nucleic acid-guided nuclease with a lowered cutting activity relative to the nucleic acid-guided nuclease having the sequence of SEQ ID No. 1, wherein the engineered nucleic acid-guided nuclease has a sequence comprising any of SEQ ID Nos. 8, 9, 10 or 15.

2. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 8.

3. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 9.

4. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 10.

5. The engineered nucleic acid-guided nuclease of claim 1 comprising SEQ ID No. 15.

6. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 .

7. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 2.

8. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 3.

9. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 11.

10. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 12.

11. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 13.

12. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 14.

13. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 67.

14. An enzyme cocktail comprising an engineered nucleic acid-guided nuclease of claim 1 and SEQ ID No. 68.

15. An enzyme cocktail comprising at least two of the engineered nucleic acid-guided nucleases of claim 1 .

16. An enzyme cocktail comprising at least three of the engineered nucleic acid-guided nucleases of claim 1 .

17. An enzyme cocktail comprising all of the engineered nucleic acid-guided nucleases of claim 1 .

Assignees

• Inscripta Inc

Inventors

• Mijts Benjamin
• Kim Juhan
• Mir Aamir
• Seamon Kyle
• Garst Andrew

Classifications

• C12N9/22 Primary

  Ribonucleases {[RNase]; Deoxyribonucleases [DNase]} · CPC title

• C12N15/10

  Processes for the isolation, preparation or purification of DNA or RNA (chemical preparation of DNA or RNA C07H21/00; preparation of non-structural polynucleotides from microorganisms or with enzymes C12P19/34) · CPC title

• C12N15/90

  Stable introduction of foreign DNA into chromosome · CPC title

• C12N15/11

  DNA or RNA fragments; Modified forms thereof (DNA or RNA not used in recombinant technology, C07H21/00); {Non-coding nucleic acids having a biological activity} · CPC title