Blood treatment with inactivation of circulating nucleic acids

Patent metadata

Field	Value
Publication number	US-11344661-B2
Application number	US-201716075679-A
Country	US
Kind code	B2
Filing date	Feb 9, 2017
Priority date	Feb 9, 2016
Publication date	May 31, 2022
Grant date	May 31, 2022

Abstract

The present invention relates to a device for the treatment of blood comprising a solid phase on which a polypeptide is immobilized which is suitable for the inactivation of free nucleic acids. Suitable polypeptides are, for example, deoxyribonucleases, ribonucleases, DNA methyltransferases or cytosine deaminases. The invention further comprises the use of such devices for the treatment of patients suffering from chronic kidney failure, cancer or lupus erythematosus, as well as methods and systems for the treatment of blood, wherein free nucleic acids are inactivated outside the body.

First claim

The invention claimed is:

1. A device for the treatment of blood, comprising:
(a) tubes for whole blood or blood plasma to flow through from and to the patient;
(b) a solid phase on which a polypeptide is immobilized which is suitable for the inactivation of free nucleic acids; and
(c) a dialyzer or haemofilter, wherein
the solid phase is upstream the dialyzer or haemofilter, or the solid phase is located within the dialyzer or haemofilter.

2. The device according to claim 1 , wherein the polypeptide is selected from the group consisting of deoxyribonucleases, ribonucleases, endonucleases, exonucleases, endoribonucleases, exoribonucleases or peptides with nuclease activity.

3. The device according to claim 1 , wherein the polypeptide is selected from the group consisting of DNA methyltransferases 1 (DNMT1) or peptides with methyltransferase activity.

4. The device according to claim 1 , wherein the polypeptide has a cytosine deaminase activity.

5. The device according to claim 1 , wherein the device furthermore comprises (d) a plasma filter.

6. The device according to claim 1 , wherein the solid phase on which the polypeptide is immobilized comprises a hollow fibre membrane, beads or a non-woven.

7. The device according to claim 1 , wherein the free nucleic acids to be inactivated have an inflammatory effect.

8. The device according to claim 1 , wherein the free nucleic acids to be inactivated are selected from the group consisting of human genomic DNA, mitochondrial DNA (mtDNA) or mRNA.

9. The device according to claim 1 , wherein the free nucleic acids to be inactivated are selected from the group consisting of bacterial or viral DNA or RNA.

10. A method for treating proinflammatory conditions in patients suffering from chronic kidney failure, cancer or lupus erythematosus, said method comprising inactivating free nucleic acids of said patients by treating blood of said patients with the device of claim 1 .

11. A method for the treatment of blood, wherein free nucleic acids are inactivated outside the body, said method comprising treating the blood with the device of claim 1 .

12. The method according to claim 11 , further comprising the step of conveying the whole blood or blood plasma of the patient through the device under conditions which make it possible to inactivate the free nucleic acids by means of the polypeptide immobilized on the solid phase.

13. The method according to claim 11 , further comprising a step of filtering the blood plasma from the whole blood.

14. The method according to claim 11 , wherein the inactivated free nucleic acids are removed from the whole blood or blood plasma by means of dialysis.

15. A system for the treatment of blood, comprising an extracorporeal circulation containing a device according to claim 1 .

16. A method for treating proinflammatory conditions in patients suffering from chronic kidney failure, cancer or lupus erythematosus, said method comprising inactivating free nucleic acids of said patients by treating blood of said patients with the system of claim 15 .

Assignees

• Fresenius Medical Care Deutschland Gmbh

Inventors

• Laubrock Andreas
• Fislage Rainer

Classifications

• A61M1/34 Primary

  Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration · CPC title

• A61M2202/203

  Bacteria · CPC title

• A61M1/36

  Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation {; Extra-corporeal blood circuits} · CPC title

• A61M1/3689

  by biological cells · CPC title

• C12N11/12

  Cellulose or derivatives thereof · CPC title