Modified foot-and-mouth disease virus 3C proteases and methods for processing FMDV P1 precursor polypeptide

Patent metadata

Field	Value
Publication number	US-11339387-B2
Application number	US-201815986214-A
Country	US
Kind code	B2
Filing date	May 22, 2018
Priority date	May 22, 2018
Publication date	May 24, 2022
Grant date	May 24, 2022

Abstract

The disclosure is directed to an isolated polynucleotide encoding a modified picornavirus 3C protease, wherein the modified picornavirus 3C protease includes an altered secondary structure and one or more amino acid substitution(s) located at one or more amino acid position(s) corresponding to positions 16-25, 99-100 and 115-130 of a wild-type Fool-and-Mouth Disease Virus (FMDV) 3C protease, wherein the isolated polynucleotide encoding the modified picornavirus 3C protease, when transformed into and co-expressed in a host cell, enhances transgene expression of a P1 precursor polypeptide in comparison to an amount of P1 precursor polypeptide transgene expression exhibited in a host cell transformed and co-expressed with a control picornavirus 3C protease, wherein the one or more corresponding amino acid position(s) is/are identified by an alignment of the modified picornavirus 3C protease with the one or more of the wild type FMDV 3C protease(s). Methods for processing a picornavirus P1 precursor polypeptide into picornavirus viral proteins and/or virus-like particles using the isolated polynucleotides are also provided.

First claim

The invention claimed is:

1. An isolated polynucleotide encoding a modified foot-and-mouth disease virus (FMDV) 3C protease,
wherein the modified FMDV 3C protease comprises a V124P amino acid substitution of a wild-type FMDV 3C protease,
wherein the modified FMDV 3C protease, when expressed in a host cell, enhances the accumulated amount of processed FMDV P1 precursor polypeptide fragments in comparison to an accumulated amount of processed FMDV P1 precursor polypeptide fragments exhibited in a host cell expressing a wild-type FMDV 3C protease, and
wherein the modified FMDV 3C protease has at least 95% identity to the wild-type FMDV 3C protease consisting of SEQ ID NO: 6.

2. The isolated polynucleotide according to claim 1 , wherein the modified FMDV 3C protease comprises
one or more amino acid substitution corresponding to amino acid positions 16-25 of a wild type FMDV 3C protease, and/or
one or more amino acid substitution corresponding to amino acid positions 99 and 100 of a wild type FMDV 3C protease.

3. The isolated polynucleotide encoding the modified FMDV 3C protease of claim 1 , wherein the modified FMDV 3C protease comprises one or more amino acid substitution at one or more position selected from the group consisting of I22 and L23.

4. The isolated polynucleotide encoding the modified FMDV 3C protease of claim 1 , wherein the modified FMDV 3C further comprises an amino acid substitution at position T100.

5. The isolated polynucleotide encoding the modified FMDV 3C protease of claim 1 , wherein said polynucleotide, when transformed into and expressed in a host cell, reduces an amount of proteolytically-cleaved eIF4A1 compared to an amount of proteolytically-cleaved eIF4A1 in a host cell expressing the wild-type FMDV 3C protease.

6. The isolated polynucleotide encoding the modified FMDV 3C protease of claim 2 , wherein at least one of the one or more amino acid substitution comprises substitution with a proline.

7. The isolated polynucleotide encoding the modified FMDV 3C protease of claim 1 , comprising at least one polynucleotide sequence encoding a P1 precursor polypeptide.

8. A vector comprising the isolated polynucleotide sequence encoding a modified FMDV 3C protease of claim 1 .

9. A host cell comprising the vector of claim 8 .

10. A composition comprising the polynucleotide encoding the modified FMDV 3C protease of claim 1 and a pharmaceutically acceptable excipient, adjuvant, buffer or solution.

11. A method for processing a FMDV P1 precursor polypeptide into FMDV viral proteins and/or Virus-Like Particles (VLPs), which method comprises:
culturing the host cell of claim 9 in a suitable medium; and
recovering at least one viral protein selected from among VP0, VP1, VP2, VP3 and VP4 and/or VLPs.

12. The isolated polynucleotide encoding the modified FMDV 3C protease of claim 1 , wherein the modified FMDV 3C protease comprises one or more amino acid substitution(s) located at one or more amino acid position(s) corresponding to positions 16-25 of the wild-type FMDV 3C protease.

13. The isolated polynucleotide encoding the modified FMDV 3C protease of claim 1 , wherein the modified FMDV 3C protease comprises one or more amino acid substitution(s) located at one or more amino acid position(s) corresponding to positions 99-100 of the wild-type FMDV 3C protease.

14. The isolated polynucleotide encoding the modified FMDV 3C protease of claim 1 , wherein the modified FMDV 3C protease comprises one or more amino acid substitution selected from the group consisting of D123P, G125P, and I128P.

15. A composition comprising the vector of claim 8 and a pharmaceutically acceptable excipient, adjuvant, buffer or solution.

16. The isolated polynucleotide encoding the modified FMDV 3C protease of claim 1 , wherein the modified FMDV 3C protease comprises one or more amino acid substitution selected from the group consisting of I22P, L23P, and T100P.

17. The isolated polynucleotide encoding the modified FMDV 3C protease of claim 1 , wherein the modified FMDV 3C protease is selected from the group consisting of SEQ ID NOS: 222, 224, 226, 230, 232, 234, and 238.

18. The isolated polynucleotide encoding the modified FMDV 3C protease of claim 1 , wherein the modified FMDV 3C protease has at least 95% identity to at least one selected from the group consisting of SEQ ID NOS: 222, 224, 226, 230, 232, 234, 236, 238, and 240.

19. The isolated polynucleotide encoding the modified FMDV 3C protease of claim 1 , wherein the wild-type FMDV 3C protease is SEQ ID NO 6.

20. The isolated polynucleotide of claim 1 wherein the encoded modified foot-and-mouth disease virus (FMDV) 3C protease has at least 95% identity to one or more wild-type FMDV 3C proteases selected from the group consisting of SEQ ID NOS: 2, 4, 10, 12, 14, 16, 18 and 20.

Assignees

• The Government Of The Us Secretary Of Homeland Security

Inventors

• Puckette Michael
• Neilan John

Classifications

• C07K14/005

  from viruses · CPC title

• C12Y304/22028

  Picornain 3C (3.4.22.28) · CPC title

• C12N9/506 Primary

  derived from RNA viruses · CPC title

• A61K39/135

  Foot- and mouth-disease virus · CPC title

• C12N15/52

  Genes encoding for enzymes or proenzymes · CPC title