Sorting biological and non-biological moieties using magnetic levitation

Patent metadata

Field	Value
Publication number	US-11338290-B2
Application number	US-201615764017-A
Country	US
Kind code	B2
Filing date	Sep 30, 2016
Priority date	Oct 2, 2015
Publication date	May 24, 2022
Grant date	May 24, 2022

Abstract

Systems and methods for levitating populations of moieties, cells, or other such units using one or more magnets in a microfluidic environment are provided. These systems and methods may be used to, for example, separate or sort heterogeneous populations of the units from one another, to assembly a multi-unit assembly during the levitating of the units, and to evaluate samples at the point of care in real-time. These systems and methods may also utilize a frame that enables an imaging device, such as a smartphone, to capture the units in real time as they are manipulated in the system.

First claim

What is claimed is:

1. A method of separating and collecting a heterogeneous population of cells, the method comprising:
obtaining a microcapillary or microfluidic channel comprising two or more outlets;
placing in the microcapillary or microfluidic channel the heterogeneous population of cells in a paramagnetic medium;
separating the heterogeneous population of cells in the microcapillary or microfluidic channel based on differences in magnetic susceptibility and density of the heterogeneous population of cells; and
withdrawing fluid comprising the separated cells from the two or more outlets using variable flow rates by microfluidic pumps at respective ones of the two or more outlets simultaneously to fractionalize the fluid comprising the separated cells across the two or more outlets by manipulation of the variable flow rates relative to one another.

2. The method according to claim 1 wherein the microcapillary or microfluidic channel is coupled to a single magnet for performing a step of levitating the heterogeneous population of cells in the microcapillary or microfluidic channel based on differences in magnetic susceptibility and density of the heterogeneous population of cells.

3. A method according to claim 2 wherein the cells are blood cells.

4. A method according to claim 3 wherein the heterogeneous population of cells include circulating tumor cells which are separated from the heterogeneous population of cells.

5. The method according to claim 1 wherein the heterogeneous population of cells comprises a blood sample with fetal cells and wherein fetal cells are separated from the blood sample in which the blood sample and a paramagnetic medium are placed in the microcapillary or microfluidic channel subjected to a magnetic field; the fetal cells are levitated within the microcapillary or microfluidic channel based on the magnetic susceptibility and density of the fetal cells; and the fetal cells are isolated using a levitation height threshold of the microcapillary or microfluidic channel.

6. A method according to claim 5 wherein the concentration of blood cells in the blood sample as provided to the microcapillary or microfluidic channel is 100,000/mL or lower.

7. A method according to claim 5 wherein the concentration of blood cells in the blood sample as provided to the microcapillary or microfluidic channel is from 0.8 to 25 million/mL.

8. A method according to claim 5 wherein the concentration of blood cells in the blood sample as provided to the microcapillary or microfluidic channel is from 50 to 250 million/mL.

9. A method according to claim 5 wherein the concentration of blood cells in the blood sample as provided to the microcapillary or microfluidic channel is from 1 to 5 million/mL.

10. A method according to claim 1 wherein the two or more outlets of the microcapillary or microfluidic channel include a top outlet and a bottom outlet.

11. A method according to claim 10 wherein a flow from the top outlet is greater than a flow from the bottom outlet.

12. A method according to claim 10 wherein a flow from the bottom outlet is greater than a flow from the top outlet.

13. A method according to claim 1 wherein the collected cells are from a blood sample.

14. A method according to claim 1 wherein the cells are collected from a saliva sample.

15. A method according to claim 1 wherein the cells are collected from a vaginal swab sample.

16. A method according to claim 1 wherein the cells are collected from an environmental sample.

17. A method according to claim 1 wherein the collected cells include cancer cells.

18. A method according to claim 1 wherein the cells are collected from a plasma sample.

19. A method according to claim 1 wherein the cells are collected from a serum sample.

Assignees

• Univ Leland Stanford Junior

Inventors

• Baday Murat
• Durmus Naside Gozde
• Calamak Semih
• Demirci Utkan
• Davis Ronald W
• Steinmetz Lars
• Yang Jaeyoung
• Chinnasamy Thiruppathiraja
• Tocchio Alessandro

Classifications

• G01N33/575

  for cancer · CPC title

• B03C2201/26

  for use in medical or biological applications · CPC title

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B03C2201/18

  Magnetic separation whereby the particles are suspended in a liquid · CPC title

• G01N2015/1486

  Counting the particles · CPC title