Maize plants with improved disease resistance

Patent metadata

Field	Value
Publication number	US-11337388-B2
Application number	US-201715586140-A
Country	US
Kind code	B2
Filing date	May 3, 2017
Priority date	May 4, 2016
Publication date	May 24, 2022
Grant date	May 24, 2022

Abstract

The present invention is in the field of plant breeding and disease resistance. More specifically, the invention includes a method for breeding corn plants containing one or more markers that are associated with resistance to fungi. The invention further includes germplasm and the use of germplasm containing at least one marker associated with resistance to Fusarium stalk rot (FSR) infection for introgression into elite germplasm in a breeding program, thus producing novel FSR resistant germplasm.

First claim

What is claimed is:

1. A method of obtaining a corn plant with enhanced Fusarium stalk rot resistance comprising:
a) providing a population of corn plants;
b) detecting in said plants the presence of a Fusarium stalk rot resistance allele at a polymorphic locus genetically linked to a chromosomal segment flanked by marker loci SEQ ID NO: 141 and SEQ ID NO: 143 on chromosome 6, wherein representative seed comprising said allele have been deposited under NCMA Accession No. 202009006;
c) selecting from said population at least a first plant based on the presence of said Fusarium stalk rot resistance allele and exhibiting enhanced Fusarium stalk rot resistance when compared to a plant lacking said allele; and
d) assaying said plant comprising said Fusarium stalk rot resistance allele for resistance to Fusarium verticilliodes, Fusarium proliferatum , or Fusarium subglutinans.

2. The method of claim 1 , wherein said polymorphic locus comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs:141-143.

3. The method of claim 1 , further defined as comprising selecting from said population at least two plants, thereby forming a population of corn plants comprising said allele and enhanced Fusarium stalk rot resistance compared to a plant lacking said allele.

4. The method of claim 1 , wherein said Fusarium stalk rot resistance allele was introgressed into said population of corn plants from a starting plant or population of corn plants containing said allele.

5. The method of claim 1 , further comprising producing a progeny plant with Fusarium stalk rot resistance from said first plant.

6. The method of claim 5 , wherein producing the progeny plant comprises marker-assisted selection for Fusarium stalk rot resistance.

7. The method of claim 5 , wherein the progeny plant is an F2-F6 progeny plant.

8. The method of claim 5 , wherein producing the progeny plant comprises backcrossing.

9. A method of producing a corn plant with enhanced Fusarium stalk rot resistance comprising:
a) crossing a first corn plant comprising a Fusarium stalk rot resistance allele with a second corn plant of a different genotype to produce one or more progeny plants;
b) selecting a progeny plant based on the presence of said allele at a polymorphic locus genetically linked to a chromosomal segment flanked by marker loci SEQ ID NO: 141 and SEQ ID NO: 143 on chromosome 6, wherein said allele confers enhanced resistance to Fusarium stalk rot compared to a plant lacking said allele, and wherein representative seed comprising said allele have been deposited under NCMA Accession No. 202009006; and
c) assaying said progeny plant comprising said allele for resistance to Fusarium verticilliodes, Fusarium proliferatum , or Fusarium subglutinans.

10. The method of claim 9 , wherein said polymorphic locus comprises a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 141-143.

11. The method of claim 9 , wherein the progeny plant is an F2-F6 progeny plant.

12. The method of claim 9 , wherein producing the progeny plant comprises backcrossing.

13. The method of claim 12 , wherein backcrossing comprises from 2-7 generations of backcrosses.

14. The method of claim 12 , wherein backcrossing comprises marker-assisted selection in at least two generations.

15. The method of claim 14 , wherein backcrossing comprises marker-assisted selection in all generations.

16. The method of claim 9 , wherein the first corn plant is an inbred or a hybrid.

17. The method of claim 9 , wherein the second corn plant is an agronomically elite corn plant.

18. The method of claim 17 , wherein the agronomically elite corn plant is an inbred or a hybrid.

Assignees

• Monsanto Technology Llc

Inventors

• Baley Jr George J
• Fouquet Romain Jean-Baptiste
• GANDHI Sonali Dilip
• Golegaonkar Prashant Ganpatrao
• Jia Hongwu
• Pan Yule
• Stein Jeffrey Michael
• Swamy Dharanendra
• Zhou Hao

Classifications

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12Q1/6895

  for plants, fungi or algae · CPC title

• A01H1/045

  using molecular markers · CPC title

• A01H1/1255 Primary

  for fungal resistance · CPC title