Lignin conversion to fuels, chemicals and materials
US-2018371502-A1 · Dec 27, 2018 · US
Conversion of biomass to useful intermediates
US11326190B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11326190-B2 |
| Application number | US-201916263867-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 31, 2019 |
| Priority date | Mar 23, 2016 |
| Publication date | May 10, 2022 |
| Grant date | May 10, 2022 |
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- Title
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Abstract
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An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.
First claim
Opening claim text (preview).
What is claimed is: 1. A non-naturally occurring Pseudomonas capable of producing 2-oxo-2H-pyran-4,6-dicarboxylic acid, comprising the deletion of genes encoding a protocatechuate 3,4-dioxygenase and further comprising insertion of genes encoding a protocatechuate 4,5-dioxygenase and a 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase wherein the Pseudomonas is capable of growing on at least one of a cellulose decomposition molecule or a lignin decomposition molecule. 2. The Pseudomonas of claim 1 , wherein the Pseudomonas is P. putida KT2440. 3. The Pseudomonas of claim 1 , wherein the cellulose decomposition molecule comprises a sugar molecule. 4. The Pseudomonas of claim 3 , wherein the sugar molecule is at least one of D-xylose or D-glucose. 5. The Pseudomonas of claim 1 , wherein the lignin decomposition molecule comprises an aromatic molecule. 6. The Pseudomonas of claim 5 , wherein the aromatic molecule is at least one of catechol, benzoate, phenol, guaiacol, protecatechuate, ferulate, p-coumarate, vanillate, or 4-hydroxybenzoate. 7. The Pseudomonas of claim 6 , wherein the aromatic molecule is protocatechuate. 8. The Pseudomonas of claim 1 , further comprising an exogenous gene encoding a carboxylase. 9. The Pseudomonas of claim 8 , wherein the exogenous gene encodes for AroY.
Assignees
Inventors
Classifications
Lyases (4.) · CPC title
2-Oxo-3-hexenedioate decarboxylase (4.1.1.77) · CPC title
Protocatechuate 3,4-dioxygenase (1.13.11.3) · CPC title
acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13) · CPC title
2-Hydroxymuconate-semialdehyde hydrolase (3.7.1.9) · CPC title
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- What does patent US11326190B2 cover?
- An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing…
- Who is the assignee on this patent?
- Alliance Sustainable Energy
- What technology area does this patent fall under?
- Primary CPC classification C12P7/44. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue May 10 2022 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 3 related publications on this page (citations in our corpus or others sharing the same primary CPC).