Barley with low levels of hordeins

Patent metadata

Field	Value
Publication number	US-11326134-B2
Application number	US-201916679461-A
Country	US
Kind code	B2
Filing date	Nov 11, 2019
Priority date	Aug 13, 2007
Publication date	May 10, 2022
Grant date	May 10, 2022

Abstract

The present invention relates to methods of producing a food or malt-base beverage suitable for consumption by a subject with Coeliac's disease. In particular, the present invention relates to methods of producing a food or malt-based beverage with low levels of hordeins. Also provided are barley plants which produce grain that can be used in the methods of the invention.

First claim

The invention claimed is:

1. A barley plant which produces barley grain comprising about 25% or less of the level of total hordeins when compared to grain from a corresponding wild-type barley plant, wherein the barley grain is homozygous for: (a) a deletion of most or all of the B-hordein encoding genes at the Hor2 locus of barley and (b) a mutation in a Lys3 gene whereby the barley grain comprising the mutation comprises 25% or less of the level of C-hordein when compared to the corresponding wild-type barley grain.

2. The barley plant of claim 1 , wherein the barley grain comprises about 15% or less of the level of total hordeins when compared to grain from a corresponding wild-type barley plant.

3. The barley plant of claim 1 , wherein the barley grain comprises about 5% or less of the level of hordeins when compared to grain from a corresponding wild-type barley plant.

4. The barley plant of claim 1 , wherein the corresponding wild-type barley plant is Bomi, Sloop, Carlsberg II, K8 or L1.

5. The barley plant of claim 1 , wherein flour produced from the barley grain comprises less than about 0.4% hordeins.

6. The barley plant of claim 1 , wherein the grain has an average weight of about 2.4 g to about 6 g.

7. The barley plant of claim 1 , wherein the grain has an average weight of about 2.4 g to about 6 g.

8. The barley plant of claim 1 , wherein the starch content of the grain is at least about 50% (w/w).

9. The barley plant of claim 1 , wherein the starch content of the grain is about 50% to about 70% (w/w).

10. The barley plant of claim 1 , wherein at least about 50% of the genome of the plant is identical to a barley cultivar Sloop.

11. The barley plant of claim 1 , which is non-transgenic.

12. The barley plant of claim 11 , wherein the barley grain comprises less than 25% of the genetic background of Riso 56 and Riso 1508.

13. The barley plant of claim 1 , wherein at least about 50% of the grain germinates within 3 days following imbibition.

14. Barley grain of the barley plant of claim 1 .

15. A method of producing barley grain, the method comprising;
a) growing the barley plant of claim 1 , and
b) harvesting the barley grain of the barley plant.

16. The method of claim 15 which comprises growing at least 10,000 plants in a field in an area of at least one hectare.

17. A method of producing flour, wholemeal, starch or other product obtained from barley grain, the method comprising;
a) obtaining the barley grain of claim 14 , and
b) processing the grain to produce the flour, wholemeal, starch or other product.

Assignees

• Commw Scient Ind Res Org
• Walter And Eliza Hall Institute Of Medical Res
• Grains Res & Dev Corp
• Melbourne Health

Inventors

• Tanner Gregory John
• Howitt Crispin Alexander

Classifications

• A23L7/25

  Fermentation of cereal malt or of cereal by malting · CPC title

• A01H5/10

  Seeds · CPC title

• C12C1/125

  Continuous or semi-continuous processes for steeping, germinating or drying · CPC title

• A23L5/20

  Removal of unwanted matter, e.g. deodorisation or detoxification · CPC title

• A23V2002/00

  Food compositions, function of food ingredients or processes for food or foodstuffs · CPC title