Device for illuminating a particle, and a system and a method for particle imaging

Patent metadata

Field	Value
Publication number	US-11307133-B2
Application number	US-201816957101-A
Country	US
Kind code	B2
Filing date	Dec 19, 2018
Priority date	Dec 22, 2017
Publication date	Apr 19, 2022
Grant date	Apr 19, 2022

Abstract

A device ( 110 ) for illuminating a particle comprises: a light waveguide ( 112; 412 a, 412 b; 512 a, 512 b ) arranged on a substrate ( 114 ); an output coupler ( 118 ) configured to output a light beam ( 150; 450 a, 450 b; 550 a, 550 b ) forming a sheet-like shape having a cross-section which has an extension in a first direction being larger than a size of a particle; and a fluidic channel ( 116; 416; 516 ) arranged on the substrate ( 114 ) for guiding a flow of particles along a longitudinal direction of the fluidic channel ( 116; 416; 516 ); wherein the sheet-like shape of the light beam ( 150; 450 a, 450 b; 550 a, 550 b ) is arranged within the fluidic channel ( 116; 416; 516 ) and the first direction of the cross-section of the light beam ( 150; 450 a, 450 b; 550 a, 550 b ) forms an angle to the longitudinal direction of the fluidic channel ( 116; 416; 516 ). A system ( 100 ) for imaging the particle comprises the device, an array ( 130; 430 a, 430 b; 530 ) of light-detecting elements ( 132; 432 a, 432 b; 532 ); and a lens ( 120 ) to converge light towards the array ( 130; 430 a, 430 b; 530 ) such that each light-detecting element ( 132; 432 a, 432 b; 532 ) detects light originating from a corresponding position in the fluidic channel ( 116; 416; 516 ).

First claim

The invention claimed is:

1. A device for illuminating a particle in a fluidic channel, the device comprising:
a light waveguide arranged on a substrate;
an output coupler configured to output light from the light waveguide to form a light beam, wherein the light beam forms a sheet-like shape such that at a distance from the output coupler the light beam has a cross-section which has an extension in a first direction being larger than a size of a particle; and
a fluidic channel arranged on the substrate for guiding a flow of particles through the fluidic channel along a longitudinal direction of the fluidic channel;
wherein the light waveguide and the fluidic channel are arranged in relation to each other such that the light beam at said distance from the output coupler is arranged within the fluidic channel, wherein the first direction of the cross-section of the light beam forms an angle to the longitudinal direction of the fluidic channel.

2. The device according to claim 1 , wherein the output coupler is configured to output light, wherein the light beam has a cross-section which has a large extension in the first direction and a small extension in a second direction, the small extension being smaller than the size of a particle.

3. The device according to claim 1 , wherein the first direction of the light beam is transverse to the longitudinal direction of the fluidic channel.

4. The device according to claim 1 , wherein the extension in the first direction of the cross-section of the light beam extends substantially across a cross-section of the fluidic channel from a first side wall of the fluidic channel to a second side wall of the fluidic channel opposite to the first side wall.

5. The device according to claim 1 , wherein the light waveguide and the fluidic channel are arranged in relation to each other such that the light beam from the output coupler enters the fluidic channel through a light entrance wall of the fluidic channel for propagating towards a light exit wall of the fluidic channel opposite to the light entrance wall, and wherein the light beam has a cross-section with a large extension and a small extension throughout the fluidic channel between the light entrance wall and the light exit wall.

6. The device according to claim 1 , wherein the device further comprises a lens arranged on the substrate, wherein the lens is configured to receive light induced by the light beam interacting with a particle in the fluidic channel and converge the received light.

7. The device according to claim 1 , wherein the light waveguide is a first light waveguide and the output coupler is a first output coupler configured to form a first light beam and wherein the device further comprises a second light waveguide and a second output coupler for forming a second light beam, which forms a sheet-like shape such that at a distance from the second output coupler the second light beam has a cross-section with a large extension in a first direction and a small extension in a second direction.

8. The device according to claim 7 , wherein the first output coupler is arranged to output the first light beam to enter the fluidic channel through a light entrance wall at a first cross-section of the fluidic channel and the second output coupler is arranged to output the second light beam to enter the fluidic channel through the light entrance wall at a second cross-section of the fluidic channel downstream to the first cross-section.

9. The device according to claim 7 , wherein the sheet-like shape of the first light beam is tilted towards the second cross-section from the light entrance wall to a light exit wall of the fluidic channel and wherein the sheet-like shape of the second light beam is tilted towards the first cross-section from the light entrance wall to the light exit wall of the fluidic channel, such that the first light beam and the second light beam will intersect at a central portion of the fluidic channel.

10. A system for particle imaging; said system comprising:
a device for illuminating a particle according to claim 1 ; and
an array of light-detecting elements; wherein the array of light-detecting elements is configured such that each light-detecting element detects light originating from a corresponding position in the fluidic channel.

11. The system according to claim 10 , wherein the system comprises a device, wherein the light waveguide is a first light waveguide and the output coupler is a first output coupler configured to form a first light beam and wherein the device further comprises a second light waveguide and a second output coupler for forming a second light beam, which forms a sheet-like shape such that at a distance from the second output coupler the second light beam has a cross-section with a lame extension in a first direction and a small extension in a second direction, wherein the array of light-detecting elements is a first array and the system further comprises a second array of light-detecting elements, wherein the first array is configured to receive light induced by the first light beam interacting with a particle in the fluidic channel and the second array is configured to receive light induced by the second light beam interacting with a particle in the fluidic channel.

12. The system according to claim 10 , further comprising a filter for removing excitation light from the light beam; and passing fluorescence light induced by the light beam.

13. The system according to claim 10 , further comprising a processing unit, which is configured to receive a sequence of recorded light intensities by the array of light-detecting elements acquired while the particle moves through the fluidic channel and to combine the received sequence for forming a two-dimensional image of the particle.

14. A method for particle imaging, said method comprising:
illuminating a particle moving through a fluidic channel, said particle being illuminated by means of a light beam that forms a sheet-like shape such that at a position within the fluidic channel, the light beam has a cross-section with a large extension in a first direction and a small extension in a second direction, the large extension being larger than a size of the particle and the small extension being smaller than the size of a particle;
receiving, light induced by the light beam interacting with a particle in the fluidic channel by an array of light-detecting elements such that each light-detecting element detects light originating from a corresponding position in the fluidic channel;
recording a received light intensity by each of the light-detecting elements in the array for forming image information of a portion of the particle.

15. The method according to claim 14 , further comprising acquiring a sequence of recorded light intensities while the particle moves through the fluidic channel, and combining the received sequence for forming a two-dimensional image of the particle.

Assignees

• Imec Vzw

Inventors

• Liu Chengxun
• Vercruysse Dries
• Verellen Niels
• Yurt Abdulkadir

Classifications

• G03H1/0465

  Particular recording light; Beam shape or geometry (G03H1/06 takes precedence) · CPC title

• G01N15/1436

  the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell · CPC title

• G01N15/147 Primary

  the analysis being performed on a sample stream · CPC title

• G03H2001/0447

  In-line recording arrangement · CPC title

• G01N2015/1454

  using phase shift or interference, e.g. for improving contrast · CPC title