Particle sorting apparatus and particle sorting method

Patent metadata

Field	Value
Publication number	US-11305318-B2
Application number	US-202117240390-A
Country	US
Kind code	B2
Filing date	Apr 26, 2021
Priority date	Apr 4, 2013
Publication date	Apr 19, 2022
Grant date	Apr 19, 2022

Abstract

A particle sorting apparatus is provided. The particle sorting apparatus includes a first detector of a first scatter channel to detect a first light scatter generated by a particle flowing in a flow channel of a fluid sample and passing through a first laser beam, and a second detector of a second scatter channel to detect a second light scatter generated by the particle flowing in the flow channel of the fluid sample and passing through a second laser beam. The particle sorting apparatus also includes circuitry configured to output a first scatter pulse information and a first detection time of the first light scatter channel, a second scatter pulse information and a second detection time of the second light scatter channel. The first laser beam and the second laser beam are irradiated to the particle in different positions of the flow channel.

First claim

The invention is claimed as follows:

1. A flow cytometry system, comprising:
a first detector of a first scatter channel to detect a first light scatter generated by a particle flowing in a flow channel of a fluid sample and passing through a first laser beam;
a second detector of a second scatter channel to detect a second light scatter generated by the particle flowing in the flow channel of the fluid sample and passing through a second laser beam; and
circuitry configured to
output a first scatter pulse information and a first detection time of the first light scatter channel, a second scatter pulse information and a second detection time of the second light scatter channel,
store the first detection time and the second detection time, and
calculate a laser delay for the particle by calculating a time difference between the second detection time and the first detection time,

wherein the first laser beam and the second laser beam are irradiated to the particle in different positions of the flow channel.

2. The flow cytometry system of claim 1 , wherein
the first light scatter and the second light scatter are one of a forward scattered light, a side scattered light, a Rayleigh scattered light and a Mie scattered light.

3. The flow cytometry system of claim 1 , wherein
the circuit is configured to associate an identifier with the detection time for each light scatter channel.

4. The flow cytometry system of claim 3 , wherein
the identifier is channel information.

5. The flow cytometry system of claim 1 , further comprising:
a first laser device to generate the first laser beam; and
a second laser device to generate the second laser beam.

6. The flow cytometry system of claim 1 , wherein
the wavelength of the first laser beam is different from the wavelength of the second laser beam.

7. The flow cytometry system of claim 1 , further comprising:
a flow path to receive a flow of the fluid sample and a flow of a sheath fluid, wherein the flow of the fluid sample is surrounded by the flow of the sheath fluid.

8. The flow cytometry system of claim 1 , wherein
the first scatter pulse information includes at least one of a width, a height, and an area of the waveform of the first light scatter, and
the second scatter pulse information includes at least one of a width, a height, and an area of the waveform of the second light scatter.

9. The flow cytometry system of claim 1 , wherein
the first detection time is calculated based on a time at a center of the waveform of the first light scatter, and
the second detection time is calculated based on a time at a center of the waveform of the second light scatter.

10. The flow cytometry system of claim 1 wherein
the circuitry is configured to determine a particle velocity of the particle based on the laser delay.

11. The flow cytometry system of claim 1 wherein
the circuitry is configured to generate a data packet for the particle, and
the data packet including the first scatter pulse information, the second scatter pulse information, and the laser delay.

12. The flow cytometry system of claim 11 , wherein
the data packet further includes at least one of the first detection time, the second detection time, or the sorting time.

13. The flow cytometry system of claim 12 , wherein
the data packet further includes at least one of an event number, trigger channel, trigger time, gate flag, or sort flag.

14. The flow cytometry system of claim 11 , wherein
the circuitry is configured to output the data packet for the particle to a storage.

15. The flow cytometry system of claim 1 , wherein
the circuitry is configured to calculate a sorting time based on the laser delay.

16. The flow cytometry system of claim 1 , wherein
the first scatter pulse information includes at least one of a width, a height, an area of the waveform, an identifier, and a time of the first light scatter, and
the second scatter pulse information includes at least one of a width, a height, an area of the waveform, an identifier, and a time of the second light scatter.

17. The flow cytometry system of claim 16 , wherein
the time of the first light scatter includes the first detection time, and
the time of the second light scatter includes the second detection time.

18. The flow cytometry system of claim 1 , wherein
the first detection time is calculated based on a time at a maximum height of the waveform of the first light scatter, and
the second detection time is calculated based on a time at a maximum height of the waveform of the second light scatter.

19. The flow cytometry system of claim 1 , further comprising:
a computer including a graphical user interface (GUI).

20. The flow cytometry system of claim 19 , wherein
the GUI is configured to chart detected data.

21. The flow cytometry system of claim 20 , wherein
the detected data includes a histogram chart or a 2D chart showing a maximum height or an area of at least one the waveform of the first light scatter and the waveform of the second light scatter.

22. A flow cytometry method comprising:
detecting, by a first detector of a first scatter channel, a first light scatter generated by a particle flowing in a flow channel of a fluid sample and passing through a first laser beam;
detecting, by a second detector of a second scatter channel, a second light scatter generated by the particle flowing in the flow channel of the fluid sample and passing through a second laser beam;
outputting a first scatter pulse information and a first detection time of the first light scatter channel, and a second scatter pulse information and a second detection time of the second light scatter channel;
storing the first detection time and the second detection time; and
calculating a laser delay for the particle by calculating a time difference between the second detection time and the first detection time,
wherein the first laser beam and the second laser beam are irradiated to the particle in different positions of the flow channel.

Assignees

• Sony Group Corp
• Sony Corp

Inventors

• Ito Tatsumi

Classifications

• F16K99/0001

  Microvalves (microdevices B81B1/00; manufacture or treatment of devices or systems in or on a substrate B81C1/00; microfluidic structures B01L3/5027; micropumps F04B19/006) · CPC title

• B07C5/3416

  according to radiation transmissivity, e.g. for light, x-rays, particle radiation (detecting hidden objects G01V) · CPC title

• B07C5/342 Primary

  according to optical properties, e.g. colour {(according to radiation transmittivity B07C5/3416)} · CPC title

• B07C5/34

  Sorting according to other particular properties {(material testing per se G01N; quality control G07C3/14)} · CPC title

• F16K99/0028

  Valves having multiple inlets or outlets · CPC title