Nanoscale motion detector

Patent metadata

Field	Value
Publication number	US-11299762-B2
Application number	US-201815957694-A
Country	US
Kind code	B2
Filing date	Apr 19, 2018
Priority date	Oct 14, 2011
Publication date	Apr 12, 2022
Grant date	Apr 12, 2022

Abstract

Motion detector comprising a flexible support (1,5) adapted to hold at least one object (6-9), a sensor (4) for measuring the displacement of said support (1) and processing means for differentiating the fluctuations of said support (1) from those induced by said object (6-9).

First claim

The invention claimed is:

1. A method for analyzing a biological object with a motion detector, the motion detector including a flexible support, a sensor for measuring fluctuations of the flexible support, and electronics for analyzing the fluctuations, the biological object consisting of bacteria or cells, in presence or absence of external or internal stimuli, the method comprising the steps of:
bringing the biological object into contact with the flexible support, the biological object imparting a nanoscopic motion,
measuring the fluctuations of the flexible support with the sensor while the biological object is in contact with the flexible support, the fluctuations being caused by the nanoscopic motion imparted by the biological object;
analyzing the statistical variance of the measured fluctuations by the electronics; and
determining a viability of the biological object based on the analysis of a statistical variance of the measured fluctuations.

2. The method according to claim 1 , wherein the step of analyzing includes calculating a statistical variance of the fluctuations of the flexible support caused by the nanoscopic motion of the biological object.

3. The method according to claim 2 , wherein in the step of analyzing, an increased statistical variance of the fluctuations indicates that the fluctuations are induced by the biological object held on the flexible support.

4. The method according to claim 1 , further comprising the step of: treating the biological object for facilitating an adherence of the biological object to the flexible support.

5. The method according to claim 1 , wherein the biological object includes a bacteria, and the nanoscopic motion includes a motion of the bacteria.

6. The method according to claim 1 , wherein the biological object is an enzyme, and the nanoscopic motion includes an interaction of the enzyme with a drug.

7. The method according to claim 1 , wherein the nanoscopic motion of the biological object is intrinsic to the biological object.

8. The method according to claim 1 , wherein in the step of analyzing, a resonance frequency of the flexible support is not taken into account.

9. The method according to claim 1 , wherein the biological object is at least one of an enzyme and a virus.

10. The method according to claim 1 , wherein the fluctuation is at least one of a vibration or a deflection.

11. A method for analyzing a biological object with a motion detector, the motion detector including a flexible support, a sensor for measuring fluctuations of the flexible support, and electronics for analyzing the fluctuations, the biological object consisting of bacteria or cells, in a presence or absence of external or internal stimuli, the method comprising the steps of:
bringing a biological object into contact with the flexible support, the biological object imparting a nanoscopic motion,
measuring the fluctuations of the flexible support with the sensor while the biological object is in contact with the flexible support, the fluctuations caused by the nanoscopic motion imparted by the biological object;
analyzing the measured fluctuations by the electronics;
wherein the step of analyzing includes calculating a statistical variance of the fluctuations of the flexible support caused by the nanoscopic motion of the biological object in order to differentiate a motion of said flexible support from the motion induced by said object; and
determining at least one of a presence and a modification of the nanoscopic motion of the biological object based on the analysis of the statistical variance.

12. The method according to claim 11 , wherein the fluctuation is at least one of a vibration or a deflection.

Assignees

• Ecole Polytechnique Fed Lausanne Epfl
• Ecole Polytechnioue Fed De

Inventors

• Kasas Sandor
• Longo Giovanni
• Dietler Giovanni
• Alonso Sarduy Livan Bladimir

Classifications

• B82Y35/00

  Methods or apparatus for measurement or analysis of nanostructures · CPC title

• G01N29/036

  by measuring frequency or resonance of acoustic waves · CPC title

• G01N29/022

  Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices (microdevices per se B81B) · CPC title

• G01N2291/0427

  Flexural waves, plate waves, e.g. Lamb waves, tuning fork, cantilever · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title