Microbial bioconversion of curcuminoids to calebin-A

Patent metadata

Field	Value
Publication number	US-11299706-B2
Application number	US-201916281120-A
Country	US
Kind code	B2
Filing date	Feb 21, 2019
Priority date	Feb 23, 2018
Publication date	Apr 12, 2022
Grant date	Apr 12, 2022

Abstract

The present invention discloses a novel endophytic fungi, Ovatospora brasiliensis MTCC 25236 for the bioconversion of curcuminoids to Calebin-A and a method for its isolation from the rhizomes of Curcuma sp. The invention also discloses a method for the bioconversion of curcuminoids to Calebin-A using an endophytic fungi Ovatospora brasiliensis MTCC 25236 and bacterial species, Acinetobacter johnsonii and Pseudomonas putida.

First claim

We claim:

1. A method for the bioconversion of curcuminoids to Calebin-A using fungal or bacterial species, said method comprising steps of:
i. culturing the fungal or bacterial species in main two batches (flasks containing batch 1 and flasks containing batch 2) in 50 ml-500 ml volumes of suitable media and incubating at 35-37° C. with shaking at 100-120 rpm for 1 to 21 days;
ii. on the 7 th day of incubation, adding varying concentrations of curcuminoids to batch 1 flasks of step i) and incubating at 37° C. with 120 rpm shaking;
iii. maintaining the batch 2 flasks of step i) without adding curcuminoids at 37° C. with 120 rpm shaking;
iv. harvesting media from batch 1 and batch 2 flasks of step ii) and step iii) after 24, 48, 72, 96, 120 hrs;
v. drying the harvested media of step iv) under vacuum and extracting using a suitable solvent;
vi. identifying the presence of Calebin-A using HPLC (high performance liquid chromatography), LC-MS (liquid chromatography-mass spectrometry) and NMR (nuclear magnetic resonance);
vii. refluxing the harvested media of step iv) with a suitable solvent followed by separating and drying the solvent layer under vacuum; and
viii. identifying the presence of Calebin-A using HPTLC, HPLC, LC-MS and NMR, wherein the fungal species is Ovatospora brasiliensis MTCC 25236, and the bacterial species is Acinetobacter johnsonii NCIMB 9871 or Pseudomonas putida NCIMB 10007.

2. The method as in claim 1 , wherein the media of step i) is selected from the group consisting of potato dextrose broth (PDB), Sabouraud Dextrose Broth, Malt Extract Broth, and Czapek Dox Broth.

3. The method as in claim 1 , wherein the solvent of step v) and vii) is selected from the group consisting of ethyl acetate, methanol, hexane, ethanol, and acetone.

4. The method as in claim 1 , wherein the solvent of step v) and vii) is ethyl acetate and methanol.

Assignees

• Majeed Muhammed
• Nagabhushanam Kalyanam
• Majeed Anju
• Thajuddin Nooruddin
• Arumugam Sivakumar
• Viswanathan Krishnamurthy Kulithalai
• Manoharan Samuel Thomas
• Ali Furqan
• Sebastian John Adams
• Moothedath Jamsheeda
• Gnanamani Muthuraman
• Beede Kirankumar
• Sami Sabinsa Group Ltd

Inventors

• Majeed Muhammed
• Nagabhushanam Kalyanam
• Majeed Anju
• Thajuddin Nooruddin
• Arumugam Sivakumar
• Viswanathan Krishnamurthy Kulithalai
• Manoharan Samuel Thomas
• Ali Furqan
• Sebastian John Adams
• Moothedath Jamsheeda
• Gnanamani Muthuraman
• Beede Kirankumar

Classifications

• A61K31/216 Primary

  of acids having aromatic rings, e.g. benactizyne, clofibrate · CPC title

• C12R2001/645

  Fungi {; Processes using fungi} · CPC title

• A61K36/9066

  Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger · CPC title

• C12N1/145 Primary

  Fungi isolates · CPC title

• C12N1/205

  Bacterial isolates · CPC title