System and method for creating tissue

Patent metadata

Field	Value
Publication number	US-11299705-B2
Application number	US-201715805790-A
Country	US
Kind code	B2
Filing date	Nov 7, 2017
Priority date	Nov 7, 2016
Publication date	Apr 12, 2022
Grant date	Apr 12, 2022

Abstract

A system and method for growing and maintaining biological material including producing a protein associated with the tissue, selecting cells associated with the tissue, expanding the cells, creating at least one tissue bio-ink including the expanded cells, printing the at least one tissue bio-ink in at least one tissue growth medium mixture, growing the tissue from the printed at least one tissue bio-ink, and maintaining viability of the tissue.

First claim

The invention claimed is:

1. A system for automatically growing tissue comprising:
a controller providing commands to the system;
a first subsystem responding to the commands, the first subsystem producing dissociated cells associated with the tissue and growth medium associated with the dissociated cells;
a second subsystem responding to the commands, the second subsystem creating the tissue based at least on the dissociated cells and the growth medium;
a third subsystem responding to the commands, the third subsystem growing the created tissue into a pre-selected mature tissue;
a fourth subsystem responding to the commands, the fourth subsystem maintaining the viability of the pre-selected mature tissue, the fourth subsystem including:
a fifth subsystem responding to the commands, the fifth subsystem receiving the tissue from the second subsystem, the fifth subsystem transmitting viability and nutrition status of the tissue;
a sixth subsystem responding to the commands, the sixth subsystem receiving the viable tissue from the fifth subsystem, the sixth subsystem incubating the viable tissue received from the fifth subsystem, at least in the growth medium received from the first subsystem, supplements, diluent, and basal media, the sixth subsystem providing viable incubated tissue; and
a seventh subsystem enabling transport of the viable incubated tissue; and

a eighth subsystem responding to the commands, the eighth subsystem enabling transport of the viable mature tissue to a patient.

2. The system as in claim 1 wherein the growth medium comprises indicators, support materials, gel, protein, and basal medium.

3. The system as in claim 2 wherein the second subsystem creates the protein.

4. The system as in claim 2 wherein the protein comprises commercially-available protein.

5. The system as in claim 1 wherein the second subsystem comprises:
a ninth subsystem responding to the commands, the ninth subsystem receiving cells, the indicators, growth medium, and the support materials, the ninth subsystem creating a bio-ink;
a tenth subsystem responding to the commands, the tenth subsystem receiving the bio-ink, the tenth subsystem printing the bio-ink; and
a eleventh subsystem responding to the commands, the eleventh subsystem receiving the printed bio-ink, the eleventh subsystem receiving the growth medium from the first subsystem, the eleventh subsystem providing the tissue to the fourth subsystem.

6. The system as in claim 1 wherein the controller comprises:
a feedback controller controlling the flow and composition of fluid to and through the tissue, the feedback controller communicating through the commands formatted according to a communications protocol, the feedback controller receiving sensed information from at least one sensor, the feedback controller basing the commands at least on the sensed information.

7. The system as in claim 6 further comprising:
a ninth subsystem cleansing the fluid after the fluid has passed through the tissue and returning the fluid to the tissue.

8. The system as in claim 5 wherein the tenth subsystem comprises:
a multi-dimensional printer printing the tissue into the cavity.

9. A system for automatically growing tissue comprising:
a controller providing commands to the system;
a first subsystem responding to the commands, the first subsystem producing dissociated cells associated with the tissue and growth medium associated with the dissociated cells, the first subsystem including:
a second subsystem responding to the commands, the second subsystem creating disassociated cells based at least on incoming cells, viral vectors, and commercial protein, the second subsystem providing the disassociated cells to a fifth subsystem;
a third subsystem responding to the commands, the third subsystem creating the growth medium based at least on indicators, support materials, carbomer, basal media, and protein; and
a fourth subsystem responding to the commands, the fourth subsystem receiving the protein from the fifth subsystem and supplying the protein to the third subsystem;

the fifth subsystem responding to the commands, the fifth subsystem creating the tissue based at least on the dissociated cells and the growth medium;
a sixth subsystem responding to the commands, the sixth subsystem growing the created tissue into a pre-selected mature tissue;
a seventh subsystem responding to the commands, the seventh subsystem maintaining the viability of the pre-selected mature tissue; and
a eighth subsystem responding to the commands, the eighth subsystem enabling transport of the viable mature tissue to a patient.

10. A system for automatically growing tissue comprising:
a controller providing commands to the system;
a first subsystem responding to the commands, the first subsystem producing dissociated cells associated with the tissue and growth medium associated with the dissociated cells;
a second subsystem responding to the commands, the second subsystem creating the tissue based at least on the dissociated cells and the growth medium;
a third subsystem responding to the commands, the third subsystem growing the created tissue into a pre-selected mature tissue;
a fourth subsystem responding to the commands, the fourth subsystem maintaining the viability of the pre-selected mature tissue; and
a fifth subsystem responding to the commands, the fifth subsystem enabling transport of the viable mature tissue to a patient;
a tissue enclosure housing the tissue, the tissue enclosure including:
a core including a cavity, the core having at least one monitoring area and at least one opening into the cavity, one of the at least one openings receiving the tissue, the core accommodating at least one material ingress and at least one material egress; and
at least one filter assembly operably coupled with the core;
wherein the tissue is confined within the cavity by the at least one filter assembly,
wherein the life of the tissue is maintained by the maintenance subsystem at least by fluid flowing through the cavity between the at least one material ingress and the at least one material egress, and
wherein the tissue is monitored through the at least one monitoring area.

11. The system as in claim 10 further comprising:
at least one plenum operably coupled with the at least one filter assembly, the at least one plenum enabling the application of pressure to the fluid and to the tissue.

12. The system as in claim 10 further comprising:
at least one heater maintaining the temperature of the tissue.

13. The system as in claim 10 wherein the growth medium surrounds the tissue.

14. The system as in claim 10 wherein the at least one filter assembly comprises:
at least one filter;
at least one filter support operably coupled with the at least one filter; and
at least one filter frame operably coupling the at least one filter and the at least one filter support with the at least one plenum.

15. The system as in claim 10 further comprising:
a tissue enclosure top removably enclosing the tissue within the core.

16. The system as in claim 10 wherein the at least one monitoring area comprises a transparent window.

17. A system for automatically growing tissue comprising:
a controller providing commands to the system;
a first subsystem responding to the commands, the first subsystem producing dissociated cells associated with the tissue and growth medium associated with the dissociated cells;
a second subsystem responding to the commands, the second subsystem creating the tissue based at least on the

Assignees

• Deka Products Lp

Inventors

• Langenfeld Christopher C
• Cannan David D B
• Van Der Merwe Dirk A
• Kamen Dean
• Demers Jason A
• Morgan Frederick
• Moreau Timothy D
• Tracey Brian D
• Ware Matthew
• Lanigan Richard J
• Baker Michael A
• Blumberg Jr David
• Gautney Richard E
• Kane Derek G
• Fawkes Dane
• Bollenbach Thomas J
• Tilley Michael C
• Jacobson Stuart A
• Mannisto John F

Classifications

• B33Y70/00

  Materials specially adapted for additive manufacturing · CPC title

• A01N1/143

  for organ perfusion · CPC title

• C12M21/08

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title

• C12N5/0018

  Culture media for cell or tissue culture (media for specific animal cell type C12N5/06) · CPC title

• C12M27/18

  Flow directing inserts · CPC title