Mixing device for mixing the content of a bioreactor, comprising a synchronization mechanism

Patent metadata

Field	Value
Publication number	US-11299704-B2
Application number	US-201615776604-A
Country	US
Kind code	B2
Filing date	Nov 16, 2016
Priority date	Nov 16, 2015
Publication date	Apr 12, 2022
Grant date	Apr 12, 2022

Abstract

The present invention relates to a system comprising a mixing device for mixing the content of a bioreactor. The system is comprising at least one movement device for initiating a mixing movement in the bioreactor or in a holder for receiving a bioreactor and at least one sensor that can be arranged in or on the bioreactor for receiving at least one physiological or physical measurement variable. The mixing device or the bioreactor further comprises a sensor or transmitter for generating a synchronization measurement variable.

First claim

What is claimed is:

1. A system comprising a mixing device for mixing of a bioreactor and a synchronization device,
the mixing device comprising:
at least one movement device for inducing a mixing motion to the bioreactor or to a holder of the bioreactor,
at least one sensor attached to or in the bioreactor for recording at least one physiological or physical parameter,
wherein the mixing device or the bioreactor further comprises a sensor for generating a synchronization readout, namely a trigger signal or a digital signal (on/off or high/low),
wherein the synchronization readout is synchronized or synchronizable with the mixing motion induced to the bioreactor or to the holder thereof,
wherein the synchronization device:
a) triggers the measurement of at least one physiological or physical parameter on the basis of the synchronization readout or
b) selects at least one recorded value of at least one physiological or physical parameter on the basis of the synchronization readout; and
wherein the system uses a frequency spectrum for calculating and/or recording the physiological or physical parameter and/or physiological or physical parameter consists of a frequency spectrum, wherein the frequency spectrum is discretely and discontinuously recorded in dependency of the synchronization readout.

2. The system of claim 1 , further comprising an evaluation device for displaying, recording and/or evaluating the at least one physiological or physical parameter.

3. The system of claim 1 , wherein a sensor for recording a physiological or physical parameter and a sensor or transmitter for generating a synchronization readout are different from one another.

4. The system of claim 1 , wherein the sensor for recording at least one physiological or physical parameter is selected from the group consisting of a capacitive sensor, an impedance sensor, a conductivity sensor, a sensor for measuring the optical density and/or turbidity, a sensor for measuring scattered light, an optical sensor for spectroscopy or absorption spectroscopy, a fluorescence sensor, an impedance spectroscopy sensor and a FTIR spectroscopy sensor.

5. The system of claim 1 , wherein the physiological or physical parameter to be recorded is selected from the group consisting of biomass, conductivity, pH value, temperature, pO 2 and/or pCO 2 , optical density, capacity, cell parameter, cell density, cell diameter, and a metabolite concentration.

6. The system of claim 1 , wherein the sensor for generating the synchronization readout is selected from the group consisting of a sensor for recording of an angle position of the mixing device, sensor for recording of data from an electronic, servo- or step motor or an electronic, servo- or step motor control, proximity sensor for monitoring the position of the bioreactor or its holder, sensor for recording of the power consumption of an electronic, servo- or step motor, acceleration sensor for recording the momentary movement change of the bioreactor or its holder, filling-level meter for measuring of a fluid level in the bioreactor, pressure, load or weight sensor, and camera system/digital image processing.

7. The system of claim 2 , wherein the synchronization device is a component of the evaluation device.

8. The system of claim 1 , wherein the frequency spectrum is generated using one or more spectroscopic processes, wherein the one or more spectroscopic processes are impedance spectroscopy or optical spectroscopy.

9. The system of claim 8 , wherein the optical spectroscopy is UV, IR, Raman or FTIR spectroscopy.

10. A method for mixing a bioreactor using the system of claim 1 .

11. A method for teaching or calibrating the system of claim 1 , the method comprising performing a test run to determine a threshold value concerning the physiological or physical parameter or the synchronization readout, wherein the synchronization readout is defined or determined, when it is exceeded or underrun, thereby triggering selection of a measured value of the physiological or physical parameter that stands in a timely connection with said exceeding or underrun of the threshold value.

12. The method of claim 11 , wherein the synchronization device computes a synchronization variable with the at least one physiological or physical measurement variable.

Assignees

• Sartorius Stedim Biotech Gmbh

Inventors

• Brütsch Simon
• Scholz Jochen

Classifications

• C12M27/16

  Vibrating; Shaking; Tilting · CPC title

• C12M41/48 Primary

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• G08C25/00

  Arrangements for preventing or correcting errors; Monitoring arrangements · CPC title

• C12M41/00

  Means for regulation, monitoring, measurement or control, e.g. flow regulation (controlling or regulating chemical, physical or physicochemical processes B01J19/0006; heating or cooling apparatus for laboratory use B01L7/00; electro optical investigation of individual particles, flow cytometers G01N15/14; automatic analysis G01N35/00; controlling or regulating in general G06N) · CPC title