Method to produce chemical pattern in micro-fluidic structure

Patent metadata

Field	Value
Publication number	US-11298697-B2
Application number	US-201916677501-A
Country	US
Kind code	B2
Filing date	Nov 7, 2019
Priority date	Sep 27, 2013
Publication date	Apr 12, 2022
Grant date	Apr 12, 2022

Abstract

A flow cell includes: a first substrate; a second substrate; a first resin layer disposed over an inner surface of the first substrate; a second resin layer disposed over an inner surface of the second substrate; a first plurality of biological capture sites located at the first resin layer; a second plurality of biological capture sites located at the second resin layer; and a polymer layer interposed between the first resin layer and the second resin layer, such that the first substrate is attached to the second substrate via at least the first resin layer, the polymer layer, and the second resin layer, wherein the polymer layer defines a plurality of microfluidic channels that extend through polymer layer.

First claim

What is claimed is:

1. A flow cell comprising:
a first substrate having an inner surface;
a second substrate having an inner surface that faces the inner surface of the first substrate;
a first resin layer disposed over the inner surface of the first substrate;
a second resin layer disposed over the inner surface of the second substrate;
a first plurality of biological capture sites located at the first resin layer;
a second plurality of biological capture sites located at the second resin layer; and
a polymer layer interposed between the first resin layer and the second resin layer, such that the first substrate is attached to the second substrate via at least the first resin layer, the polymer layer, and the second resin layer, wherein the polymer layer defines a plurality of microfluidic channels that extend through polymer layer.

2. The flow cell of claim 1 , comprising a plurality of recesses in which the first plurality of biological capture sites and the second plurality of biological capture sites are disposed.

3. The flow cell of claim 1 , wherein each of the plurality of biological capture sites comprises an adhesive promoter and a bio-medium.

4. The flow cell of claim 3 , wherein the bio-medium is a hydrogel.

5. The flow cell of claim 3 , wherein the adhesive promotor is a silane-based adhesive promotor.

6. The flow cell of claim 3 , wherein the adhesive promotor comprises hexamethyldisilazane or 3-aminopropyl triethoxysilane.

7. The flow cell of claim 1 , wherein a thickness of the first substrate and a thickness of the second substrate are approximately 1 mm or less.

8. The flow cell of claim 1 , wherein one or both of the first substrate and the second substrate are transparent.

9. The flow cell of claim 8 , wherein one or both of the first substrate and the second substrate are made of glass.

10. The flow cell of claim 1 , wherein the first plurality of biological capture sites are offset from the second plurality of biological capture sites in a top view.

11. The flow cell of claim 1 , wherein the first plurality of biological capture sites are aligned with the second plurality of biological capture sites in a top view.

12. The flow cell of claim 1 , wherein a through hole extends through the first substrate and the first resin layer.

Assignees

• Illumina Inc

Inventors

• Tsai Shang-Ying
• Hung Li-Min
• Peng Jung-Huei
• Bowen Shane
• Han Hui
• Chan Danny
• Park Sang

Classifications

• B01L3/502707 Primary

  characterised by the manufacture of the container or its components · CPC title

• B01L2300/0636

  Integrated biosensor, microarrays · CPC title

• B01L2300/0887

  Laminated structure · CPC title

• B32B2307/412

  Transparent · CPC title

• B01L3/502715

  characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces · CPC title