Media and fermentation methods for producing polysaccharides in bacterial cell culture
US-2019292513-A1 · Sep 26, 2019 · US
Mammalian cell culture
US11292829B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11292829-B2 |
| Application number | US-201214127050-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 29, 2012 |
| Priority date | Jul 1, 2011 |
| Publication date | Apr 5, 2022 |
| Grant date | Apr 5, 2022 |
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Abstract
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The invention provides a method for culturing mammalian cells. The method provides greater control over cell growth to achieve high product titer cell cultures by changing the temperature of the cell culture and/or by starving the cells in their asparagine supply.
First claim
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What is claimed is: 1. A method of inducing growth arrest in a mammalian cell culture such that cells stop increasing in number, as measured by viable cell density (VCD), wherein the mammalian cells are Chinese Hamster Ovary (CHO) cells, comprising: establishing a mammalian cell culture in a serum-free culture medium in a bioreactor, wherein the mammalian cell culture is established by inoculating the bioreactor with at least 0.5×10 6 to 3.0×10 6 cells/mL; perfusing said cultu…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12N5/0682Primary
Chemistry & Metallurgy · mapped topic
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Frequently asked questions
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- What does patent US11292829B2 cover?
- The invention provides a method for culturing mammalian cells. The method provides greater control over cell growth to achieve high product titer cell cultures by changing the temperature of the cell culture and/or by starving the cells in their asparagine supply.
- Who is the assignee on this patent?
- Follstad Brian D, Mccoy Rebecca E, Morris Arvia E, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0682. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Apr 05 2022 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 1 related publication on this page (citations in our corpus or others sharing the same primary CPC).