Apparatus for performing contactless optically-induced dielectrophoresis for separation of circulating tumor cells

Patent metadata

Field	Value
Publication number	US-11292012-B2
Application number	US-201916577765-A
Country	US
Kind code	B2
Filing date	Sep 20, 2019
Priority date	Apr 19, 2016
Publication date	Apr 5, 2022
Grant date	Apr 5, 2022

Abstract

A method for performing contactless ODEP for separation of CTCs is provided with the steps of obtaining patients' blood with rare cell suspected CTCs; adding at least one fluorescent antibody binding to CTCs into the blood; staining the blood; injecting the stained blood with fluorescent dye into an ODEP device and then performing fluorescent image identification; trapping the CTCs with at least one fluorescent antibody in the ODEP device by creating an image pattern and then generating an ODEP force; Separating the trapped CTCs from other non-CTCs cells; absorbing the trapped CTCs; and obtaining a high purity of CTCs. An apparatus for performing contactless ODEP for separation of CTCs is also provided.

First claim

What is claimed is:

1. An apparatus for performing contactless optically-induced dielectrophoresis (ODEP) for separation of circulating tumor cells, the apparatus comprising: an ODEP device including a first conductive glass, a bio-compatible membrane, and a second conductive glass wherein the bio-compatible membrane is disposed below the first conductive glass, the bio-compatible membrane includes a transverse main channel and a longitudinal micro channel perpendicular to the main channel and joining the main channel at a cell separation zone; the first conductive glass includes a first hole for receiving a sample and a second hole for discharging an exhaust aligned with two ends of the main channel respectively, and a third hole for collecting a target aligned with one end of the micro channel, the second conductive glass being disposed below the bio-compatible membrane, a first electrode channel disposed along an edge of the first conductive glass and corresponding to the main channel, and a second electrode channel perpendicular to the first electrode channel, joining the first electrode channel, and corresponding to the micro channel; and a controller including an optical projection device and an image fetch device.

2. The apparatus of claim 1 , further comprising a light guide layer formed on a bottom of the second conductive glass, the light guide layer being distal the first conductive glass and configured to guide light.

3. The apparatus of claim 1 , wherein the ODEP device includes a fluid driver and a signal generation device connected to the fluid driver.

Assignees

• Univ Chang Gung
• Ace Medical Tech Co Ltd

Inventors

• Wu Min-Hsien

Classifications

• B03C2201/26

  for use in medical or biological applications · CPC title

• C12M47/04

  Cell isolation or sorting (purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04) · CPC title

• B01L2400/0424

  Dielectrophoretic forces · CPC title

• B03C5/005 Primary

  Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength · CPC title

• C12N2509/00

  Methods for the dissociation of cells, e.g. specific use of enzymes · CPC title