Evaporation-controlling container inserts

Patent metadata

Field	Value
Publication number	US-11291965-B2
Application number	US-201916570647-A
Country	US
Kind code	B2
Filing date	Sep 13, 2019
Priority date	Mar 14, 2013
Publication date	Apr 5, 2022
Grant date	Apr 5, 2022

Abstract

An apparatus configured for mixing the contents of one or more fluid containers includes a fluid container support platform configured to hold one or more fluid containers. The fluid container support platform is configured to index the container to one or more specified locations and to be moved in an orbital path about an orbital center independently of the rotation about the central axis of rotation. The apparatus further includes an indexing drive system configured to effect indexing movement of the container support platform and a vortex drive system configured to effect powered orbital movement of the container support platform about the orbital center. An evaporation limiting insert placed within containers reduces exposure of the fluid contents of the container to atmospheric air, thereby reducing susceptibility of the fluid contents to evaporation.

First claim

The invention claimed is:

1. A method for removing fluid contents of a container while minimizing evaporation of the fluid contents from the container, the method comprising:
a) inserting an evaporation-limiting insert into an opening of a neck of the container, the evaporation-limiting insert comprising a hollow tubular body configured to extend into the container from the neck of the container, the tubular body including a plurality of holes formed through a wall of the tubular body and distributed along at least a portion of the length of the tubular body, wherein the holes are situated above a bottom edge of the tubular body, and wherein a majority of the holes are located below a midpoint of the tubular body, and
b) after step a), inserting a portion of a fluid transfer apparatus through the opening of the container and into the tubular body of the evaporation-limiting insert and withdrawing a portion of the fluid contents from the container with the fluid transfer apparatus.

2. The method of claim 1 , wherein the fluid transfer apparatus comprises a robotic pipettor, and step b) comprises inserting a pipette tip associated with the robotic pipettor through the opening of the container and into the tubular body of the evaporation-limiting insert and aspirating a portion of the fluid contents from the container with the robotic pipettor.

3. The method of claim 2 , further comprising performing liquid level sensing with the robotic pipettor.

4. The method of claim 1 , further comprising, prior to step b), a step of agitating the fluid contents of the container to promote mixing of the fluid contents, whereby the holes formed through the wall of the tubular body of the insert permit fluid to flow through a space inside the tubular body.

5. The method of claim 4 , wherein the fluid contents comprise solid supports, wherein each of the holes is sized to permit passage of the solid supports therethrough, and wherein the agitating step is performed to keep the solid supports in suspension.

6. The method of claim 4 , wherein agitating the fluid contents of the container comprises agitating the container.

7. The method of claim 1 , wherein the surface of the fluid contents within the container is above the top-most one of the holes formed through the wall of the tubular body.

8. The method of claim 1 , further comprising a step of determining the height of the fluid contents within the container with an apparatus configured to detect a fluid surface inserted through the opening of the container and into the tubular body of the evaporation-limiting insert.

9. The method of claim 1 , wherein the fluid contents comprise solid supports, and wherein each of the holes is sized to permit passage of the solid supports therethrough, and further comprising a step of agitating the fluid contents of the container to keep the solid supports in suspension.

10. The method of claim 9 , wherein the solid supports are magnetically-responsive particles or beads.

11. The method of claim 1 , wherein the tubular body has a length extending from the opening of the container to a bottom surface of the interior of the container.

12. The method of claim 11 , wherein the bottom edge of the tubular body is configured to form one or more gaps between the bottom edge and the bottom surface of the container.

13. The method of claim 11 , wherein the tubular body of the evaporation-limiting insert comprises one or more slots extending axially from the bottom edge to thereby form one or more gaps between the bottom edge and the bottom surface of the container.

14. The method of claim 1 , wherein the evaporation-limiting insert further comprises a retainer feature at an upper end portion of the tubular body that is configured to engage an inner portion of the neck of the container to secure the insert within the container.

15. The method of claim 14 , wherein the retainer feature comprises a detent engaged with an inside surface of the container.

16. The method of claim 14 , wherein the retainer feature comprises two or more outwardly splayed resilient tabs formed at a top portion of the tubular body and pressing resiliently against an inside surface of the container.

17. The method of claim 16 , wherein the tabs are separated by slits extending lengthwise in a wall of the tubular body from a top edge thereof, wherein the length of each slit is longer than the neck of the container, so that the slit extends below the neck of the container.

18. The method of claim 1 , wherein the holes through the evaporation-limiting insert are axially aligned along a side of the tubular body.

19. The method of claim 1 , wherein the holes are arranged in two groups axially aligned along opposed sides of the tubular body.

20. The method of claim 19 , wherein each group of axially aligned holes comprises at least three holes.

21. The method of claim 20 , wherein each group of axially aligned holes comprises no more than six holes.

22. The method of claim 1 , wherein the tubular body is substantially cylindrical and has a substantially constant diameter along its entire length.

23. The method of claim 1 , wherein the tubular body is tapered such that an outside dimension of the tubular body progressively decreases moving away from the opening of the container.

24. The method of claim 1 , wherein all of the holes are situated below the midpoint of the tubular body.

Assignees

• Gen Probe Inc

Inventors

• Knight Byron J

Classifications

• B01F31/201

  Holders therefor · CPC title

• B01F31/22 Primary

  with supporting means moving in a horizontal plane, e.g. describing an orbital path for moving the containers about an axis which intersects the receptacle axis at an angle · CPC title

• B65D23/00

  Details of bottles or jars not otherwise provided for · CPC title

• B01L3/523 Primary

  with means for closing or opening · CPC title

• G01N2035/00524

  Mixing by agitating sample carrier · CPC title