Anti-galectin-7 antibody, kit comprising the same, and uses thereof

Patent metadata

Field	Value
Publication number	US-11286304-B2
Application number	US-201916653986-A
Country	US
Kind code	B2
Filing date	Oct 15, 2019
Priority date	Oct 15, 2018
Publication date	Mar 29, 2022
Grant date	Mar 29, 2022

Abstract

Provided herein is a novel antibody exhibiting binding affinity and specificity to galectin-7. Also provided herein are methods for treating psoriasis or cancer in a subject by administering to the subject a drug selected with the aid of the present anti-galectin-7 antibody.

First claim

What is claimed is:

1. An antibody or a fragment thereof that binds to galectin-7, comprising:
a heavy chain variable region comprising a first heavy chain complementarity determining region (CDR-H1), a second heavy chain CDR (CDR-H2), and a third heavy chain CDR (CDR-H3), wherein the CDR-H1, the CDR-H2, and the CDR-H3 respectively comprise the amino acid sequences of SEQ ID NOs: 1, 2 and 3; and
a light chain variable region comprising a first light chain CDR (CDR-L1), a second light chain CDR (CDR-L2), and a third light chain CDR (CDR-L3), wherein the CDR-L1, the CDR-L2, and the CDR-L3 respectively comprise the amino acid sequences of SEQ ID NOs: 4, 5 and 6.

2. The antibody or the fragment thereof of claim 1 , wherein the heavy chain variable region is at least 85% identical to SEQ ID NO: 7, and the light chain variable region is at least 85% identical to SEQ ID NO: 8.

3. The antibody or the fragment thereof of claim 2 , wherein the heavy chain variable region has the amino acid sequence of SEQ ID NO: 7, and the light chain variable region has the amino acid sequence of SEQ ID NO: 8.

4. A method of selecting a drug candidate suitable for treating psoriasis or cancer in a subject, comprising,
(a) incubating keratinocytes with one or more candidate drugs;
(b) determining the expression level of galectin-7 in the keratinocytes of step (a) by use the antibody or the fragment thereof of claim 1 ; and
(c) selecting the drug candidate from the one or more candidate drugs based on the expression level determined in step (b), wherein the drug candidate increases the expression level of galectin-7.

5. The method of claim 4 , wherein the selected drug candidate in the step (c) is a statin.

6. The method of claim 5 , wherein the subject has the psoriasis, and the statin is fluvastatin, atorvastatin, cerivastatin, pitavastatin, or simvastatin.

7. The method of claim 5 , wherein the subject has the cancer, and the statin is fluvastatin, atorvastatin, cerivastatin, pitavastatin, simvastatin, lovastatin, mevastatin, pravastatin, or rosuvastatin.

8. The method of claim 4 , wherein the heavy chain variable region of the antibody or the fragment thereof is at least 85% identical to SEQ ID NO: 7, and the light chain variable region of the antibody or the fragment thereof is at least 85% identical to SEQ ID NO: 8.

9. The method of claim 8 , wherein the heavy chain variable region of the antibody or the fragment thereof has the amino acid sequence of SEQ ID NO: 7, and the light chain variable region of the antibody or the fragment thereof has the amino acid sequence of SEQ ID NO: 8.

10. A method of treating psoriasis or cancer in a subject, comprising:
(1) selecting a drug candidate suitable for treating the psoriasis or the cancer by,
(1a) incubating keratinocytes with one or more candidate drugs;
(1b) determining the expression level of galectin-7 in the keratinocytes of step (1a) by use the antibody or the fragment thereof of claim 1 ; and
(1c) selecting the drug candidate from the one or more candidate drugs based on the expression level determined in step (1b), wherein the drug candidate increases the expression level of galectin-7; and

(2) treating the subject by administering to the subject an effective amount of a pharmaceutical composition, which comprises the selected drug candidate in the step (1c) and a pharmaceutically acceptable carrier.

11. The method of claim 10 , wherein the selected drug candidate in the step (1c) is a statin.

12. The method of claim 11 , wherein the subject has the psoriasis, and the statin is fluvastatin, atorvastatin, cerivastatin, pitavastatin, or simvastatin.

13. The method of claim 11 , wherein the subject has the cancer, and the statin is fluvastatin, atorvastatin, cerivastatin, pitavastatin, simvastatin, lovastatin, mevastatin, pravastatin, or rosuvastatin.

14. The method of claim 10 , wherein the subject has the psoriasis, and the pharmaceutical composition further comprises an TNF-α inhibitor, which is an anti-TNF-α antibody or a TNF-α antagonist.

15. The method of claim 10 , wherein the subject has the cancer, and the pharmaceutical composition further comprises a renin-angiotensin system (Ras) inhibitor, which is farnesyl thiosalicylic acid (FTS), ARS-853, or ARS-162.

16. The method of claim 10 , wherein the subject is a human.

17. The method of claim 10 , wherein the cancer is bladder cancer, biliary cancer, bone cancer, brain tumor, breast cancer, cervical cancer, colorectal cancer, dysgerminoma, esophageal cancer, epidermal cancer, gastric cancer, gastrointestinal stromal tumor (GIST), glioma, non-Hodgkin's lymphoma, head and neck cancer, intestinal cancer, Kaposi's sarcoma, liver cancer, lung cancer, lymphoma, lymphoid leukemia, melanoma, myeloid leukemia, nasopharyngeal cancer, oral cancer, ovary cancer, pancreatic cancer, prostate cancer, retinoblastoma, renal cell carcinoma, sarcoma, seminoma, skin cancer, spleen cancer, squamous cell carcinoma, teratoma, teratocarcinoma, thyroid cancer, or thyroid follicular cancer.

18. The method of claim 17 , wherein the cancer is esophageal cancer, lung cancer, oral cancer, or skin cancer.

Assignees

• Academia Sinica

Inventors

• Liu Fu-Tong
• Chen Hung-Lin
• Chiang Po-Cheng

Classifications

• A61P17/06

  Antipsoriatics · CPC title

• G01N2800/205

  Scaling palpular diseases, e.g. psoriasis, pytiriasis · CPC title

• G01N33/5044 Primary

  involving specific cell types · CPC title

• C07K2317/56

  variable (Fv) region, i.e. VH and/or VL · CPC title

• G01N2333/705

  Assays involving receptors, cell surface antigens or cell surface determinants · CPC title