Chimeric insecticidal proteins toxic or inhibitory to Lepidopteran pests

Patent metadata

Field	Value
Publication number	US-11286284-B2
Application number	US-201916658938-A
Country	US
Kind code	B2
Filing date	Oct 21, 2019
Priority date	Oct 16, 2014
Publication date	Mar 29, 2022
Grant date	Mar 29, 2022

Abstract

Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

First claim

What is claimed is:

1. A chimeric insecticidal protein comprising the amino acid sequence as set forth in SEQ ID NO: 50, wherein the chimeric insecticidal protein exhibits inhibitory activity against an insect species of the order Lepidoptera.

2. A polynucleotide encoding the chimeric insecticidal protein of claim 1 , wherein the polynucleotide is operably linked to a heterologous promoter and the chimeric insecticidal protein comprises the amino acid sequence as set forth in SEQ ID NO: 50.

3. A polynucleotide encoding the chimeric insecticidal protein of claim 1 , wherein the polynucleotide comprises a nucleotide sequence that encodes the chimeric insecticidal protein comprising the amino acid sequence as set forth in SEQ ID NO: 50.

4. A host cell comprising the polynucleotide set forth in claim 2 , wherein the host cell is selected from the group consisting of a bacterial host cell or a plant host cell.

5. The host cell of claim 4 , wherein the bacterial host cell is selected from the group consisting of Agrobacterium, Rhizobium, Bacillus, Brevibacillus, Escherichia, Pseudomonas, Klebsiella , and Erwinia ; and wherein the Bacillus species is a Bacillus cereus or a Bacillus thuringiensis , said Brevibacillus is a Brevibacillus laterosperous , and said Escherichia is an Escherichia coli.

6. The host cell of claim 4 , wherein the plant host cell is selected from the group of plants consisting of monocots and dicots.

7. An insect inhibitory composition comprising the chimeric insecticidal protein of claim 1 .

8. The insect inhibitory composition of claim 7 , further comprising at least one insect inhibitory agent different from the chimeric insecticidal protein.

9. The insect inhibitory composition of claim 8 , wherein the at least one insect inhibitory agent is selected from the group consisting of an insect inhibitory protein, an insect inhibitory dsRNA molecule, and an insect inhibitory toxic agent.

10. The insect inhibitory composition of claim 8 , wherein the at least one other insect inhibitory agent exhibits activity against one or more pest species of the orders Lepidoptera, Coleoptera, Hemiptera, Homoptera, or Thysanoptera.

11. A seed comprising:
a) an insect inhibitory effective amount of a chimeric insecticidal protein comprising the amino acid sequence as set forth in SEQ ID NO: 50; or
b) a polynucleotide as set forth in SEQ ID NO: 49.

12. A method of controlling a Lepidopteran pest, the method comprising contacting the Lepidopteran pest with an inhibitory amount of the chimeric insecticidal protein of claim 1 .

13. A transgenic plant cell, plant or plant part comprising a chimeric insecticidal protein, wherein:
a) the chimeric insecticidal protein comprises the amino acid sequence as set forth in SEQ ID NO: 50; or
b) the chimeric insecticidal protein comprises an amino acid sequence having having at least 95% identity to SEQ ID NO: 50.

14. A method of controlling a Lepidopteran pest, comprising exposing the pest to the transgenic plant cell, plant or plant part of claim 13 , wherein said plant cell, plant or plant part expresses a Lepidopteran inhibitory amount of the chimeric insecticidal protein.

15. A commodity product derived from the plant cell, plant, or plant part of claim 13 , wherein the product comprises a detectable amount of the chimeric insecticidal protein.

16. The commodity product of claim 15 , wherein the product is selected from the group consisting of plant biomass, oil, meal, animal feed, flour, flakes, bran, lint, hulls, and processed seed.

17. A method of producing a seed comprising the chimeric insecticidal protein of claim 1 , the method comprising:
a) planting at least one seed comprising the chimeric insecticidal protein of claim 1 ;
b) growing plants from the seed; and
c) harvesting seed from the plants, wherein the harvested seed comprises the chimeric insecticidal protein of claim 1 .

18. A recombinant polynucleotide molecule encoding the chimeric insecticidal protein of claim 1 , comprising the nucleotide sequence of SEQ ID NO: 49; and a polynucleotide sequence encoding an insect inhibitory agent different from the chimeric insecticidal protein.

19. A recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide segment encoding a chimeric insecticidal protein, wherein:
a) the chimeric insecticidal protein comprises the amino acid sequence set forth in SEQ ID NO: 50; or
b) the chimeric insecticidal protein comprises an amino acid sequence having having at least 95% identity to SEQ ID NO: 50.

Assignees

• Monsanto Technology Llc

Inventors

• Baum James A
• Cerruti Thomas A
• Dart Crystal L
• English Leigh H
• Flasinski Stanislaw
• Fu Xiaoran
• Guzov Victor M
• Howe Arlene R
• Morgenstern Jay P
• Roberts James K
• Salvador Sara
• Wang Jinling Hung

Classifications

• C07K14/325 Primary

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• A01N37/46

  N-acyl derivatives · CPC title

• C07K2319/00

  Fusion polypeptide · CPC title

• C12N15/8286

  for insect resistance · CPC title

• C12N15/62

  DNA sequences coding for fusion proteins · CPC title