Thermocycling of a block comprising multiple sample

Patent metadata

Field	Value
Publication number	US-11285488-B2
Application number	US-201816058135-A
Country	US
Kind code	B2
Filing date	Aug 8, 2018
Priority date	Apr 4, 2005
Publication date	Mar 29, 2022
Grant date	Mar 29, 2022

Abstract

The present invention relates to the field of high throughput analysis of samples. In particular, the present invention is directed to a device, a System and a method for simultaneous tempering of multiple samples. More particular, the invention relates to the simultaneous thermocycling of multiple samples to perform PCR in a microtiter plate format.

First claim

The invention claimed is:

1. A method for simultaneous thermocycling of multiple samples, the method comprising:
a) providing a device comprising a thermal block, a heat sink, a first liquid-vapor equalization thermal base, a second liquid-vapor equalization thermal base, a computer, and at least two thermoelectric based heat pumps actively controlled by the computer, wherein the device is configured such that the first thermal base is in thermal contact with and sandwiched directly in-between the heat pumps and the heat sink and the second thermal base in thermal contact with and sandwiched directly in-between the thermal block and the heat pumps;
b) performing a thermocycling protocol with the computer, said performing comprising
actively controlling the heat pumps to alternatively heat and cool the thermal block and at the same time to reverse direction of heat transfer through the first and second thermal bases, and
independently varying the heat conducting properties of the first thermal base and the second thermal base during the thermocycling protocol, wherein the first thermal base is configured to aid a cooling procedure by distributing heat to be dissipated homogenously across an entire surface of the heat sink, and the second thermal base is configured to aid a heating procedure by distributing heat generated at the heat pumps homogeneously across the thermal block, wherein the thermal block comprises a shape defined by a pair of sidewall outer surfaces and a bottom surface disposed therebetween, and the second thermal base comprises a corresponding shape comprising inner surfaces sized and shaped to thermally contact the pair of sidewall outer surfaces and the bottom surface of the thermal block.

2. The method according to claim 1 , comprising controlling the power supply to the at least two heat pumps and the independently varying first and second switches with the computer.

3. The method according to claim 1 , wherein independently varying is effectuated via a first switch on the first thermal base and a second switch on the second thermal base.

4. The method according to claim 1 comprising independently varying the heat conducting properties of the first thermal base via the first switch by changing volume and/or flow rate within the first thermal base.

5. The method according to claim 1 comprising independently varying the heat conducting properties of the second thermal base via the second switch by changing volume and/or flow rate within the second thermal base.

6. The method according to claim 1 wherein the thermocycling protocol comprises nucleic acid amplification.

7. The method according to claim 1 , wherein the first thermal base, the second thermal base, the heat sink, and the thermal block each have a cross section area, the cross section area of the first thermal base being less than 20% larger than the cross section area of the heat sink, wherein the cross section area of the second thermal base is larger than the cross section area of the thermal block, wherein the cross section areas are in parallel to respective contact areas, such that heat transfer to and from the first and second thermal bases comprises homogenous heat transfer across the cross-sectional areas of the heat sink and thermal block, respectively.

8. A method for the simultaneous thermocycling of multiple samples comprising the steps:
a) providing a thermal block with multiple recesses, at least two heat pumps, a first thermal base comprising a vapor chamber device for transporting and distributing heat, a second thermal base, a heat sink, and a control unit, arranged such that the first thermal base is between and in thermal contact with the heat sink and at least two heat pumps, the heat pumps situated between the first thermal base and the second thermal base, the second thermal base being in thermal contact with the thermal block, wherein thermal contact is effectuated by one or more of a paste having a high thermal conductance, a thermally conductive foil, and a mechanical force;
b) placing the multiple samples within the recesses of the thermal block; and
c) performing a thermocycling protocol with the control unit, wherein the control unit actively controls the heat pumps and independently controls a heat conducting property of the first thermal base and a heat conducting property of the second thermal bases, wherein the first thermal base is configured to aid a cooling procedure by distributing heat to be dissipated homogenously across an entire surface of the heat sink, and the second thermal base is configured to aid a heating procedure by distributing heat generated at the heat pumps homogeneously across the thermal block, wherein the thermal block comprises a shape defined by a pair of sidewall outer surfaces and a bottom surface disposed therebetween, and the second thermal base comprises a corresponding shape comprising inner surfaces sized and shaped to thermally contact the pair of sidewall outer surfaces and the bottom surface of the thermal block.

9. The method according to claim 8 , wherein the first thermal base is substantially planar and free of recesses.

10. The method according to claim 9 , wherein the cross sectional area of the first thermal base is less than 20% larger or smaller than the cross sectional area of the heat sink, and wherein the cross-sectional area of the first thermal base is larger than the cross sectional area of the thermal block and said cross sectional areas aligned parallel to the respective contact areas.

11. The method according to claim 8 wherein the second thermal base is substantially planar and free of recesses.

12. The method according to claim 8 wherein the thermocycling protocol comprises nucleic acid amplification.

Assignees

• Roche Molecular Systems Inc

Inventors

• Schlaubitz Thomas
• Burdack Torsten
• Federer Paul
• George Christian
• Grueter Guido
• Scholle Andreas
• Tenzler Guenter

Classifications

• C12M1/38 Primary

  Temperature-responsive control · CPC title

• B01L7/52 Primary

  with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples · CPC title

• B01L2300/1855

  using phase changes in a medium · CPC title

• B01L2300/1822

  using Peltier elements · CPC title

• B01L2300/0829

  Multi-well plates; Microtitration plates · CPC title