Separating agent

Patent metadata

Field	Value
Publication number	US-11285458-B2
Application number	US-201414903699-A
Country	US
Kind code	B2
Filing date	Jul 8, 2014
Priority date	Jul 8, 2013
Publication date	Mar 29, 2022
Grant date	Mar 29, 2022

Abstract

A separating medium for hydrophilic interaction chromatography useful in separating hydrophilic compounds. The hydrophilic interaction chromatography separating medium, which is formed from a support and a ligand carried by the support, is a separating medium wherein the ligand is a (meth)acrylic polymer having a constituent unit derived from the compound indicated by formula (I).

First claim

The invention claimed is:

1. A separating medium for hydrophilic interaction chromatography, being formed from a support and a ligand supported on the support, wherein
the ligand is supported on the support by physical adsorption or chemical bonding, and
the ligand is a (meth)acrylic polymer having a constituent unit derived from a compound represented by Formula (I) below:

in Formula (I), there are one or two double bonds between atoms making up the heterocyclic ring, X 1 is selected from the group consisting of S, SCH 3 + , O, NH, NCH 3 , CH 2 , CHR and CR 1 R 2 , each of X 2 , X 3 and X 4 is selected from the group consisting of N, NH, NCH 3 , CH 2 , CHR, NCH 3 + , CH, CR and CR 1 R 2 , wherein each of R 1 and R 2 is an optionally substituted C 1-18 alkyl, C 6-18 aryl, C 2-18 alkenyl, C 2-18 alkynyl, C 7-18 aralkyl, C 2-18 acyl, C 3-18 cycloalkyl, carboxyl, amino, C 6-18 aryloxy or C 1-18 alkoxy, halo, hydroxyl, nitro or cyano group, while R is a substituted or non-substituted C 1-18 alkyl, C 6-18 aryl, C 2-18 alkenyl, C 2-18 alkynyl, C 7-18 aralkyl, C 2-18 acyl, C 3-18 cycloalkyl, carboxyl, amino, C 6-18 aryloxy or C 1-18 alkoxy, halo, hydroxyl, nitro or cyano group, at least two of X 1 , X 2 , X 3 and X 4 are not CH 2 , CH, CR or CR 1 R 2 , and R 3 is H or CH 3 .

2. The separating medium according to claim 1 , wherein the ligand is a (meth)acrylic polymer having a constituent unit derived from a compound selected from the group consisting of aminoimidazole, aminoimidazoline, aminotriazole, aminotriazole, aminotetrazole, aminothiadiazole and aminomethylimidazole.

3. The separating medium according to claim 1 , wherein the ligand is a (meth)acrylic polymer having a constituent unit derived from aminotetrazole.

4. The separating medium according to claim 1 , wherein the ligand is a methacrylic polymer having a constituent unit derived from aminotetrazole.

5. The separating medium according to claim 1 , wherein the support is a silica gel or silica monolith.

Assignees

• Nat Univ Corp Kyoto Inst Technology
• Daicel Corp

Inventors

• Ikegami Tohru
• Kawachi Yusuke
• Kunisawa Akihiro

Classifications

• B01J2220/82

  Shaped bodies, e.g. monoliths, plugs, tubes, continuous beds · CPC title

• B01J20/286 Primary

  Phases chemically bonded to a substrate, e.g. to silica or to polymers · CPC title

• C08F220/606

  and containing other heteroatoms · CPC title

• C08F20/54

  Amides {, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide} · CPC title

• B01D15/305

  Hydrophilic interaction chromatography [HILIC] · CPC title