High rebaudioside C-content stevia plant

Patent metadata

Field	Value
Publication number	US-11284576-B2
Application number	US-201716473094-A
Country	US
Kind code	B2
Filing date	Dec 26, 2017
Priority date	Dec 27, 2016
Publication date	Mar 29, 2022
Grant date	Mar 29, 2022

Abstract

An objective of the present invention is to provide a Stevia plant having a high content of rebaudioside C. The present invention provides a high rebaudioside C-content type, non genetically modified Stevia plant that has a higher content of rebaudioside C than wild-type Stevia varieties, more specifically at least 20% higher content. The present invention further provides: a method for producing such a high rebaudioside C-content type, non genetically modified Stevia plant; and dried leaves obtained from such a plant.

First claim

The invention claimed is:

1. A high rebaudioside C-content non-genetically modified Stevia plant comprising a rebaudioside C content of 15% by weight or more in a dried leaf thereof, and comprising the A>T polymorphism at the 60th nucleotide of SEQ ID NO: 1 in the genome.

2. A seed of the plant according to claim 1 .

3. A dried leaf of the plant according to claim 1 .

4. A tissue culture or a cultured plant cell of the plant according to claim 1 , wherein said tissue culture or cultured plant cell comprises said polymorphism.

5. The tissue culture or the cultured plant cell according to claim 4 , which is an embryo, a meristem cell, a pollen, a leaf, a root, a root apex, a petal, a protoplast, a leaf section and a callus.

6. A method of producing a high rebaudioside C-content Stevia plant comprising a rebaudioside C content of 15% by weight or more in a dried leaf thereof, the method comprising crossing a Stevia plant according to claim 1 with a second Stevia plant.

7. The method according to claim 6 , wherein the second plant is the Stevia plant according to claim 1 .

8. A method of producing a rebaudioside C-containing extract, comprising obtaining an extract from the plant according to claim 1 .

9. A method of producing rebaudioside C, comprising purifying rebaudioside C from the rebaudioside C-containing extract according to claim 8 .

10. A method of producing a medicament, a flavor or a food or beverage, comprising mixing the extract obtained by the method according to claim 8 with other component(s).

11. A method of screening for a high rebaudioside C-content Stevia plant, comprising identifying the A>T polymorphism at the 60th nucleotide of SEQ ID NO: 1 in the genome of a test plant.

12. The method according to claim 11 , further comprising measuring a content of rebaudioside C in a leaf tissue.

13. A method of producing a rebaudioside C-containing extract, comprising obtaining an extract from the dried leaf according to claim 3 .

14. A method of producing rebaudioside C, comprising purifying rebaudioside C from the rebaudioside C-containing extract according to claim 13 .

15. A method of producing a medicament, a flavor or a food or beverage, comprising mixing the extract obtained by the method according to claim 13 with other component(s).

16. A method of producing a medicament, a flavor or a food or beverage, comprising mixing rebaudioside C obtained by the method according to claim 14 with other component(s).

Assignees

• Suntory Holdings Ltd

Inventors

• Iwaki Kazunari
• Ono Eiichiro
• HIRAI Tadayoshi
• Miyagawa Katsuro

Classifications

• C12N5/04

  Plant cells or tissues {(culture media C12N5/0025)} · CPC title

• A61K8/60

  Sugars; Derivatives thereof · CPC title

• A61K36/28

  Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea · CPC title

• A23L33/105

  Plant extracts, their artificial duplicates or their derivatives · CPC title

• A01H5/10 Primary

  Seeds · CPC title