Mechanisms and methods for liquid sample introduction into a chromatography system

Patent metadata

Field	Value
Publication number	US-11280769-B2
Application number	US-201916678454-A
Country	US
Kind code	B2
Filing date	Nov 8, 2019
Priority date	Nov 9, 2018
Publication date	Mar 22, 2022
Grant date	Mar 22, 2022

Abstract

The disclosure relates to a system for liquid sample introduction into a chromatography system. The system includes a syringe, a first valve in fluid communication with the syringe, a second valve in fluid communication with the sample, a vessel located between, and in fluid communication with, the first and second valves, a third valve in fluid communication with the first valve, the second valve and a chromatography column, and a pump in fluid communication with the third valve and a mobile phase. When the valves are in a first position the syringe draws the sample into the vessel. The mobile phase flows to the chromatography column. When the valves are in a second position, a portion of the mobile phase flows into the vessel, mixing with and pressurizing the sample. When the valves are in a third position, the mixed and pressurized sample flows to the chromatography column.

First claim

What is claimed is:

1. A system for liquid sample introduction into a chromatography system, the system comprising:
a sample syringe for drawing up a sample;
a first multi-port valve in fluid communication with the sample syringe;
a second multi-port valve in fluid communication with the sample;
a vessel located between, and in fluid communication with, the first and second multi-port valves;
a third multi-port valve in fluid communication with the first multi-port valve, the second multi-port valve and a chromatography column; and
a pump in fluid communication with the third multi-port valve and a mobile phase;
wherein (1) when the first multi-port valve, the second multi-port valve, and the third multi-port valve are in a first position, the sample syringe draws the sample into the vessel and the mobile phase flows to the chromatography column; (2) when the first multi-port valve, the second multi-port valve, and the third multi-port valve are in a second position, at least a portion of the mobile phase flows into the vessel, mixing with and pressurizing the sample; and (3) when the first multi-port valve, the second multi-port valve, and the third multi-port valve are in a third position, the mixed and pressurized sample flows to the chromatography column.

2. The system of claim 1 , further comprising a tee in fluid communication with the third multi-port valve, the second multi-port valve, and the column.

3. The system of claim 1 , wherein the first multi-port valve, the second multi-port valve and the third multi-port valve are rotary valves, solenoid valves, or pneumatic valves.

4. The system of claim 1 , wherein the first multi-port valve, the second multi-port valve and the third multi-port valve have four ports, six ports, eight ports, or ten ports.

5. The system of claim 1 , wherein the first multi-port valve has a different number of ports than the second multi-port valve.

6. The system of claim 1 , wherein the mobile phase is a highly-compressible solvent.

7. The system of claim 6 , wherein the highly-compressible solvent is carbon dioxide.

8. The system of claim 1 , wherein the chromatography system is a supercritical fluid chromatography system.

9. A method for introducing a liquid sample into a chromatography system, the method comprising:
drawing the liquid sample through a second valve by actuating a syringe in fluid communication with a first valve, thereby partially filling a vessel with the liquid sample;
flowing a mobile phase through a third valve into a chromatography column;
flowing a first portion of the mobile phase into the chromatography column and flowing a second portion of the mobile phase into the vessel by adjusting the second valve, thereby mixing and pressurizing the liquid sample and the mobile phase; and
injecting the mixed and pressurized liquid sample and mobile phase into the chromatography column by adjusting the first valve, the second valve and the third valve.

10. The method of claim 9 , wherein the vessel is positioned between the first and second valves.

11. The method of claim 9 , wherein drawing the liquid sample through a second valve and flowing a mobile phase through a third valve into a chromatography column are performed simultaneously.

12. The method of claim 9 , wherein the mobile phase is a highly-compressible solvent.

13. The method of claim 12 , wherein the highly-compressible solvent is carbon dioxide.

14. A system for liquid sample introduction into a chromatography system, the system comprising:
a sample syringe for drawing up a sample;
a first multi-port valve in fluid communication with the sample syringe and the sample;
a second multi-port valve in fluid communication with the first multi-port valve and a vessel;
a third multi-port valve in fluid communication with the second multi-port valve and a chromatography column; and
a pump in fluid communication with the third multi-port valve and a mobile phase;
wherein (1) when the first multi-port valve, the second multi-port valve, and the third multi-port valve are in a first position, the sample syringe draws the sample and the mobile phase flows to the chromatography column; (2) when the first multi-port valve, the second multi-port valve, and the third multi-port valve, are in a second position, the sample flows from the sample syringe to the vessel and the mobile phase continues to flow to the chromatography column; (3) when the first multi-port valve, the second multi-port valve, and the third multi-port valve are in a third position at least a portion of the mobile phase flows into the vessel, mixing with and pressurizing the sample; and (4) when the first multi-port valve, the second multi-port valve, and the third multi-port valve are in a fourth position, the mixed and pressurized sample flows to the chromatography column.

15. The system of claim 14 , further comprising a tee in fluid communication with the third multi-port valve, the second multi-port valve, and the column.

16. The system of claim 14 , wherein the first multi-port valve, the second multi-port valve and the third multi-port valve are rotary valves, solenoid valves or pneumatic valves.

17. The system of claim 14 , wherein the first multi-port valve, the second multi-port valve and the third multi-port valve have four ports, six ports, eight ports, or ten ports.

18. The system of claim 14 , wherein the first multi-port valve has a different number of ports than the second multi-port valve.

19. The system of claim 14 , wherein the mobile phase is a highly-compressible solvent.

20. The system of claim 19 , wherein the highly-compressible solvent is carbon dioxide.

21. The system of claim 14 , wherein the chromatography system is a supercritical fluid chromatography system.

Assignees

• Waters Technologies Corp

Inventors

• Hill Jason
• Tarafder Abhijit
• Fogwill Michael O
• Mcdonald Thomas
• Cormier Sylvain

Classifications

• G01N2030/201

  multiport valves, i.e. having more than two ports · CPC title

• G01N30/32

  of pressure or speed (G01N30/36 takes precedence) · CPC title

• G01N30/20 Primary

  using a sampling valve · CPC title

• G01N2030/202

  rotary valves · CPC title

• G01N2030/326

  pumps · CPC title