Bioprocess container having an optical measuring device

Patent metadata

Field	Value
Publication number	US-11280730-B2
Application number	US-201917285961-A
Country	US
Kind code	B2
Filing date	Aug 5, 2019
Priority date	Oct 31, 2018
Publication date	Mar 22, 2022
Grant date	Mar 22, 2022

Abstract

The invention relates to a bioprocess container (10) having an optical measuring device (100) for non-invasive spectroscopic measurement comprising: a container housing (12), a port housing (102), which is connected to the container housing (12) and is sealed off with respect to the interior (18) of the container housing (12); at least one radiation-emitting element (124), which is designed to transmit electromagnetic radiation through the at least one fluid contained in the container housing (12); at least one radiation-receiving element (126), which is designed to at least partly receive the radiation which was transmitted by the radiation-emitting element (124); and at least one measuring insert (122), which holds and supports the at least one radiation-emitting element (124) and/or the at least one radiation-receiving element (126).

First claim

The invention claimed is:

1. A bioprocess container with an optical measuring device for noninvasive spectroscopic measurement, comprising:
a container housing which is suitable for accommodating at least one fluid to be measured;
a port housing which is connected to the container housing and is closed off from the interior of the container housing;
at least one radiation-emitting element which is designed to transmit electromagnetic radiation through the at least one fluid contained in the container housing;
at least one radiation-receiving element which is designed to at least partially receive the radiation which was emitted by the radiation-emitting element; and
at least one measuring insert which holds or supports the at least one radiation-emitting element and/or the at least one radiation-receiving element,
wherein the measuring insert is at least partially pushable into the port housing and is detachably connected to the port housing when pushed in.

2. The bioprocess container as claimed in claim 1 , wherein the port housing comprises at least one window which is designed to at least partially allow electromagnetic radiation to pass between the interior of the container housing and an interior of the port housing.

3. The bioprocess container as claimed in claim 1 , wherein the port housing has at least one measuring gap or measuring recess into which the fluid to be measured is flowable from the interior of the container housing.

4. The bioprocess container as claimed in claim 3 , wherein the measuring gap has at least two opposing windows which are spaced apart from one another such that the measuring gap is formed between the windows.

5. The bioprocess container as claimed in claim 4 , wherein a radiation deflection device is connected to the measuring insert and is pushable into the port housing together with the measuring insert,
wherein the radiation deflection device has at least one first deflection element and at least one second deflection element,
wherein the first and second deflection elements are arranged such that they are spaced apart by the at least two windows and the measuring gap.

6. The bioprocess container as claimed in claim 3 , wherein the measuring gap is formed by a radiation deflection device,
wherein the radiation deflection device has at least one first deflection element and at least one second deflection element,
wherein the first and second deflection elements are arranged opposite one another and are at least regionally spaced apart from one another, so that the measuring gap is formed between the first and second deflection elements.

7. The bioprocess container as claimed in claim 6 , wherein the first deflection element is designed to receive electromagnetic radiation which is emitted by the radiation-emitting element and to then deflect it to the second deflection unit such that the radiation passes through the measuring gap, and
wherein the second deflection unit is designed to receive the radiation from the first deflection unit and to then deflect it to the radiation-receiving element.

8. The bioprocess container as claimed in claim 6 , wherein the first deflection element is designed to receive electromagnetic radiation which is emitted by the radiation-emitting element and to then deflect it to the measuring gap, and
wherein a radiation-receiving element is arranged below the measuring gap.

9. The bioprocess container as claimed in claim 6 , wherein a face of the second deflection element that faces the measuring gap is at least regionally diffusely scattering,
wherein the first deflection element is designed to receive electromagnetic radiation which is emitted by the radiation-emitting element and to then deflect it to the measuring gap and/or the diffusely scattering face of the second deflection element, and
wherein the radiation-receiving element is arranged such that it can measure the electromagnetic radiation which is reflected by the fluid to be measured and/or the diffusely scattering face.

10. The bioprocess container as claimed in claim 6 , wherein the first and second deflection elements are each a prism or each have a radiation-reflecting face.

11. The bioprocess container as claimed in claim 6 , wherein the first and second deflection elements are arranged on at least one window.

12. The bioprocess container as claimed in claim 1 , wherein the radiation-emitting element and the radiation-receiving element are arranged at the same position below the measuring gap.

13. The bioprocess container as claimed in claim 1 , wherein the measuring insert has at least one holding recess into which the radiation-emitting element or the radiation-receiving element is insertable.

14. The bioprocess container as claimed in claim 13 , wherein the measuring insert comprises:
at least one holding face in which the at least one holding recess is formed and which is arranged opposite a window, a first deflection element and/or a second deflection element when insertion into the port housing has taken place, and
at least one connecting face which is connected to the at least one holding face and is designed to be connected to the port housing when insertion has taken place.

15. The bioprocess container as claimed in claim 1 , wherein the measuring insert is clampable into the port housing, lockable into place with the port housing and/or screwable to the port housing.

16. The bioprocess container as claimed in claim 1 , wherein the bioprocess container is a bioreactor.

17. The bioprocess container as claimed in claim 1 , wherein the port housing at least partially protrudes into the interior of the container housing.

18. An optical measuring device for noninvasive spectroscopic measurement for a bioprocess container, comprising:
a port housing which is connectable to a container housing of the bioprocess container and is designed such that it is closed off from the interior of the container housing when connected;
at least one radiation-emitting element which is designed to transmit electromagnetic radiation through at least one fluid contained in the container housing;
at least one radiation-receiving element which is designed to at least partially receive the radiation which was emitted by the radiation-emitting element; and
at least one measuring insert which holds or supports the at least one radiation-emitting element and/or the at least one radiation-receiving element,
wherein the measuring insert is at least partially pushable into the port housing and is detachably connected to the port housing when pushed in.

Assignees

• Sartorius Stedim Biotech Gmbh

Inventors

• Höhse Marek
• Grimm Christian
• Regen Thomas

Classifications

• G01N2021/513

  Cuvettes for scattering measurements · CPC title

• G01N21/55

  Specular reflectivity · CPC title

• C12M31/08

  by conducting or reflecting elements located inside the reactor or in its structure · CPC title

• G01J3/14

  using refracting elements, e.g. prisms (G01J3/18, G01J3/26 take precedence {prisms per se G02B5/04}) · CPC title

• G01N2021/0325

  Cells for testing reactions, e.g. containing reagents · CPC title