Chemical compositions and methods of using same

Patent metadata

Field	Value
Publication number	US-11279969-B2
Application number	US-201916559755-A
Country	US
Kind code	B2
Filing date	Sep 4, 2019
Priority date	Nov 21, 2016
Publication date	Mar 22, 2022
Grant date	Mar 22, 2022

Abstract

The present disclosure relates to chemical compositions, kits, and apparatuses and methods for using these compositions, kits and apparatuses in various assays.

First claim

What is claimed is:

1. A probe comprising:
a primary nucleic acid molecule hybridized to at least six secondary nucleic acid molecules,
wherein the primary nucleic acid molecule comprises a first domain and a second domain, wherein the at least six secondary nucleic acid molecules are hybridized to the second domain of the primary nucleic acid molecule,
wherein the primary nucleic acid molecule comprises at least one photocleavable linker located between the first domain and the second domain,
wherein each of the secondary nucleic acid molecules is hybridized to at least five tertiary nucleic acid molecules,
wherein each secondary nucleic acid molecule comprises a first domain and a second domain, wherein the first domain is hybridized to the primary nucleic acid molecule and the second domain is hybridized to the at least five tertiary nucleic acid molecules,
wherein each of the secondary nucleic acid molecules comprise at least one photocleavable linker located between its first domain and second domain,
wherein each of the tertiary nucleic acid molecules comprises at least one detectable label such that the probe comprises at least 30 detectable labels.

2. The probe of claim 1 , wherein the first domain of the primary nucleic acid comprises about 14 nucleotides.

3. The probe of claim 2 , wherein the primary nucleic acid comprises about 80 to about 100 nucleotides.

4. The probe of claim 1 , wherein each secondary nucleic acid molecule comprises about 80 to about 90 nucleotides.

5. The probe of claim 1 , wherein each tertiary nucleic acid molecule comprises about 10 to about 20 nucleotides.

6. The probe of claim 5 , wherein each tertiary nucleic acid molecule comprises about 15 nucleotides.

7. The probe of claim 1 , wherein all of the at least 30 detectable labels have the same emission spectra.

8. The probe of claim 1 , wherein at least one of the at least 30 detectable labels has a first emission spectra and at least one of the at least 30 detectable labels has a second emission spectra.

9. The probe of claim 8 , wherein the first emission spectra and the second emission spectra are spectrally resolvable.

10. The probe of claim 1 , wherein at least one detectable label is a fluorescent label.

11. A probe comprising:
a primary nucleic acid molecule hybridized to only six secondary nucleic acid molecules,
wherein the primary nucleic acid molecule comprises a first domain and a second domain, wherein the six secondary nucleic acid molecules are hybridized to the second domain of the primary nucleic acid molecule,
wherein the primary nucleic acid molecule comprises at least one photocleavable linker located between the first domain and the second domain,
wherein each of the secondary nucleic acid molecules is hybridized to only five tertiary nucleic acid molecules,
wherein each secondary nucleic acid molecule comprises a first domain and a second domain, wherein the first domain is hybridized to the primary nucleic acid molecule and the second domain is hybridized to the five tertiary nucleic acid molecules,
wherein each of the secondary nucleic acid molecules comprise at least one photocleavable linker located between its first domain and second domain,
wherein each of the tertiary nucleic acid molecules comprises at least one detectable label such that the probe comprises at least 30 detectable labels.

12. The probe of claim 11 , wherein the first domain of the primary nucleic acid comprises about 14 nucleotides.

13. The probe of claim 12 , wherein the primary nucleic acid comprises about 80 to about 100 nucleotides.

14. The probe of claim 13 , wherein each secondary nucleic acid molecule comprises about 80 to about 90 nucleotides.

15. The probe of claim 14 , wherein each tertiary nucleic acid molecule comprises about 10 to about 20 nucleotides.

16. The probe of claim 15 , wherein each tertiary nucleic acid molecule comprises about 15 nucleotides.

17. The probe of claim 16 , wherein the probe comprises 30 detectable labels.

18. The probe of claim 17 , wherein all of the 30 detectable labels have the same emission spectra.

19. The probe of claim 17 , wherein at least one of the 30 detectable labels has a first emission spectra and at least one of the 30 detectable labels has a second emission spectra, wherein the first emission spectra and the second emission spectra are spectrally resolvable.

Assignees

• Nanostring Technologies Inc

Inventors

• Dunaway Dwayne L
• Manrao Elizabeth A
• Beechem Joseph M
• Khafizov Rustem
• Korukonda Sanghamithra
• Deng Yi
• Kim Dae
• Gregory Mark
• Hoang Margaret

Classifications

• C12Q1/6816

  characterised by the detection means (C12Q1/6804 takes precedence) · CPC title

• C12Q1/6825 Primary

  Nucleic acid detection involving sensors · CPC title

• C12Q1/6874 Primary

  involving nucleic acid arrays, e.g. sequencing by hybridisation · CPC title

• C12Q2565/101

  Interaction between at least two labels · CPC title

• C12Q1/6869 Primary

  Methods for sequencing · CPC title