Sample processing systems and methods

Patent metadata

Field	Value
Publication number	US-11278883-B2
Application number	US-201716474942-A
Country	US
Kind code	B2
Filing date	Dec 29, 2017
Priority date	Dec 30, 2016
Publication date	Mar 22, 2022
Grant date	Mar 22, 2022

Abstract

The disclosure features methods, fluid delivery platforms, and apparatus for preparing a sample on a substrate that includes a substrate handler configured to move a substrate between a first position and a second position, and a platform positioned so that when the substrate is in the second position, the platform faces the substrate, where the platform includes a fluid delivery area having a second surface formed from a hydrophilic material for which a water contact angle is 40 degrees or less, and a first surface facing the substrate when the substrate is in the second position, formed from a hydrophobic material for which a water contact angle is 100 degrees or more.

First claim

What is claimed is:

1. An apparatus for preparing a sample on a substrate, comprising:
a substrate handler configured to move a substrate between a first position and a second position; and
a platform positioned so that when the substrate is in the second position, the platform faces the substrate,
wherein the platform comprises:
a fluid delivery area having a second surface formed from a hydrophilic material for which a water contact angle is 40 degrees or less; and
a first surface facing the substrate when the substrate is in the second position, formed from a hydrophobic material for which a water contact angle is 100 degrees or more.

2. The apparatus of claim 1 , wherein the platform comprises a first member formed of the hydrophilic material, and a layer of the hydrophobic material disposed on at least a portion of the first member to form the first surface.

3. The apparatus of claim 2 , wherein a thickness of the hydrophobic material layer measured in a direction perpendicular to a plane defined by the first surface is 100 microns or less.

4. The apparatus of claim 3 , wherein the thickness of the hydrophobic material layer is 5 microns or less.

5. The apparatus of claim 1 , wherein the hydrophilic material comprises at least one material selected from the group consisting of mica, glass, and glass-ceramic composite materials.

6. The apparatus of claim 1 , wherein the hydrophilic material comprises at least one material selected from the group consisting of metals and metal oxides.

7. The apparatus of claim 1 , wherein the hydrophilic material comprises an oxidized product of the hydrophobic material.

8. The apparatus of claim 1 , wherein the hydrophobic material comprises at least one material selected from the group consisting of polydimethylacrylamide, polydimethylsiloxane, polyolefins, and fluoropolymers.

9. The apparatus of claim 1 , further comprising at least one spacer positioned on the first surface so that when the substrate is in the second position, the substrate contacts the at least one spacer, and a maximum variation in a closest distance between the substrate and the first surface for any two locations on the first surface is 10 microns or less.

10. The apparatus of claim 1 , further comprising a fluid outlet port positioned on the second surface.

11. The apparatus of claim 10 , wherein a center of the fluid outlet port is displaced from a central axis of the fluid delivery area along a direction orthogonal to the central axis.

12. The apparatus of claim 10 , further comprising first and second fluid inlet ports positioned on the second surface on opposite sides of a central axis of the fluid delivery area, wherein for each of the first and second fluid inlet ports:
a flow axis extends between a center of the fluid inlet port and the fluid outlet port; and
an angle between the flow axis and the central axis of the recess is α.

13. The apparatus of claim 12 , further comprising third and fourth fluid inlet ports positioned on the second surface on opposite sides of the central axis, wherein for each of the third and fourth fluid inlet ports:
a flow axis extends between a center of the fluid inlet port and the fluid outlet port; and
an angle between the flow axis and the central axis of the recess is β.

14. The apparatus of claim 13 , wherein β is 10 degrees or less.

15. The apparatus of claim 14 , wherein β is 6 degrees or less.

16. The apparatus of claim 13 , wherein α is larger than β.

17. The apparatus of claim 12 , wherein α is 15 degrees or less.

18. The apparatus of claim 17 , wherein α is 10 degrees or less.

19. The apparatus of claim 12 , wherein the second surface is planar.

20. The apparatus of claim 19 , wherein the fluid delivery area forms a recess in the first surface, and wherein the recess comprises lateral wall surfaces that extend between the first and second surfaces.

21. The apparatus of claim 20 , wherein each pair of adjacent lateral wall surfaces is oriented so that an angle subtended by the surfaces is more than 90 degrees.

22. The apparatus of claim 21 , wherein each pair of adjacent lateral wall surfaces blend along a rounded fillet.

23. The apparatus of claim 20 , wherein the recess comprises:
parallel first and second lateral wall surfaces;
a third lateral wall surface orthogonal to the first and second lateral wall surfaces;
fourth and fifth lateral wall surfaces extending between the third lateral wall surface and the first and second lateral wall surfaces, respectively; and
sixth and seventh lateral wall surfaces that extend from the first and second lateral wall surfaces, respectively, and that intersect.

24. The apparatus of claim 23 , wherein the bottom surface of the recess has a heptagonal transverse shape, and wherein at least some of the lateral wall surfaces of the recess have different lengths.

25. The apparatus of claim 23 , wherein the first, second, third, and fourth fluid inlet ports are positioned closer to the third lateral wall surface than to the sixth and seventh lateral wall surfaces.

26. The apparatus of claim 1 , wherein a maximum depth of the fluid delivery area, measured along a direction perpendicular to the upper surface, is 200 microns or less.

27. The apparatus of claim 26 , wherein the maximum depth is 150 microns or less.

28. The apparatus of claim 1 , wherein a difference between the water contact angles of the hydrophobic material and the hydrophilic material is 50 degrees or more.

29. The apparatus of claim 28 , wherein the difference between the water contact angles of the hydrophobic material and the hydrophilic material is 70 degrees or more.

Assignees

• Roche Diagnostics Hematology Inc
• Roche Diagnostics Operations Inc

Inventors

• Ergezen Ertan
• Conroy Stephen
• Oosterbroek Rijk Edwin
• Cherubini Claudio

Classifications

• B01L2400/0406

  capillary forces · CPC title

• G01N1/312

  for samples mounted on planar substrates · CPC title

• B01L3/0293 Primary

  for liquids · CPC title

• G01N35/00029

  provided with flat sample substrates, e.g. slides (G01N35/028 takes precedence) · CPC title

• B01L9/52

  Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips · CPC title