Compositions of polymeric nanowires and methods of making and using thereof

Patent metadata

Field	Value
Publication number	US-11278626-B2
Application number	US-201716338103-A
Country	US
Kind code	B2
Filing date	Oct 4, 2017
Priority date	Oct 5, 2016
Publication date	Mar 22, 2022
Grant date	Mar 22, 2022

Abstract

Aspects of the present disclosure include compositions having a plurality of individual polymeric nanowires. Individual polymeric nanowires according to embodiments include a bioactive compound. Methods for preparing and methods for administering the subject compositions of individual polymeric nanowires to a subject are also described. Kits having one or more components for practicing the subject methods are also provided.

First claim

What is claimed is:

1. A method for preparing a composition comprising individual polymeric nanowires, the method comprising:
applying a polymer composition comprising a polymer to a surface of a substrate to produce a polymer-coated substrate;
positioning a template structure comprising a plurality of pores onto the polymer-coated substrate;
heating the polymer-coated substrate in a manner sufficient to melt the polymer and draw the polymer into the pores of the template structure until the template structure comes into direct contact with the substrate;
removing the template structure from the substrate, wherein the template structure comprises formed polymeric nanowires; and
etching the removed template structure to dissolve the template structure and produce a composition comprising individual polymeric nanowires, wherein the individual polymeric nanowires are discrete nanowires and are not connected together.

2. The method according to claim 1 , wherein etching the template structure comprises dissolving the template structure with a base.

3. The method according to claim 2 , wherein the template structure is broken up into a plurality of pieces and the plurality of pieces are dissolved in the base.

4. The method according to claim 2 , wherein the base is sodium hydroxide.

5. The method according to claim 1 , further comprising flattening the polymer-coated substrate prior to positioning the template structure on the polymer-coated substrate.

6. The method according to claim 5 , wherein flattening comprises removing solvent from the polymer composition.

7. The method according to claim 5 , wherein flattening comprises heating the polymer-coated substrate.

8. The method according to claim 1 , wherein the polymer-coated substrate is heated to a temperature that is from 5° C. to 100° C. above the melting temperature of the polymer.

9. The method according to claim 1 , wherein pressure is applied to the template structure to draw the polymer into the pores of the template structure.

10. The method according to claim 1 , wherein the template structure comprises a porous membrane.

11. The method according to claim 10 , wherein the porous membrane is a porous metal membrane.

12. The method according to claim 11 , wherein the porous membrane is a porous metal oxide membrane.

13. The method according to claim 12 , wherein the porous membrane is a porous aluminum oxide membrane.

14. The method according to claim 1 , further comprising purifying the composition comprising individual polymeric nanowires.

15. The method according to claim 14 , wherein purifying comprises sonicating the composition and filtering the composition comprising individual polymeric nanowires with deionized water or a buffer solution.

16. The method according to claim 1 , wherein the polymer comprises a polyester.

17. The method according to claim 16 , wherein the polymer comprises polycaprolactone.

18. The method according to claim 1 , wherein the polymer has a molecular weight of from 5 kDa to 250 kDa.

19. The method according to claim 18 , wherein the polymer is polycaprolactone having a molecular weight of from 40 kDa to 80 kDa.

20. The method according to claim 1 , wherein the individual polymeric nanowires have a diameter of from 10 nm to 500 nm.

21. The method according to claim 20 , wherein the individual polymeric nanowires have a diameter of about 200 nm.

22. The method according to claim 1 , wherein the individual polymeric nanowires have a length of from 1 μm to 50 μm.

23. The method according to claim 22 , wherein the individual polymeric nanowires have a length of from 10 μm to 20 μm.

24. The method according to claim 1 , wherein the aspect ratio of the individual polymeric nanowires is 2 or higher.

25. The method according to claim 24 , wherein the aspect ratio of the individual polymeric nanowires is 5 or higher.

26. The method according to claim 1 , wherein the individual polymeric nanowires do not have a lumen.

27. The method of claim 2 , wherein the template structure with the formed polymeric nanowires is shaken, vortexed or sonicated while in the base to release the polymeric nanowires into solution.

28. The method of claim 1 , wherein removing the template structure comprises using a blade to remove the template structure from the substrate.

29. The method of claim 28 , further comprising allowing the polymer to cool overnight prior to removing the template structure from the substrate.

Assignees

• Univ California

Inventors

• Zamecnik Colin R
• Desai Tejal A

Classifications

• A61K47/593 Primary

  Polyesters, e.g. PLGA or polylactide-co-glycolide · CPC title

• B82Y5/00

  Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery · CPC title

• B82Y40/00

  Manufacture or treatment of nanostructures · CPC title

• C07K16/00

  Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies · CPC title

• C07K16/246

  IL-2 · CPC title