Protein variant generation by region shuffling
US-9593326-B2 · Mar 14, 2017 · US
Engineered deoxyribose-phosphate aldolases
US11274286B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-11274286-B2 |
| Application number | US-201916460118-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 2, 2019 |
| Priority date | Jul 9, 2018 |
| Publication date | Mar 15, 2022 |
| Grant date | Mar 15, 2022 |
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- Title
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Abstract
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The present invention provides engineered deoxyribose-phosphate aldolase polypeptides useful under industrial process conditions for the production of pharmaceutical and fine chemical compounds.
First claim
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We claim: 1. An engineered deoxyribose-phosphate aldolase comprising a polypeptide sequence having at least 95% sequence identity to SEQ ID NO: 2 or a functional fragment thereof, wherein said engineered deoxyribose-phosphate aldolase comprises a substitution in said polypeptide sequence of X145C/R/K, and wherein said engineered deoxyribose-phosphate aldolase has at least 1.2 fold increased activity on (R)-2-ethynyl-glyceraldehyde as compared to the wild-type polypeptide sequence of SEQ ID NO:2, and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO: 2. 2. The engineered deoxyribose-phosphate aldolase of claim 1 , wherein said engineered deoxyribose-phosphate aldolase comprises a polypeptide sequence that is at least 95% identical to the sequence of at least one engineered deoxyribose-phosphate aldolase variant set forth in the even numbered sequences of SEQ ID NOS: 6, 12, 42, 104, or 140-478. 3. The engineered deoxyribose-phosphate aldolase of claim 1 , wherein said engineered deoxyribose-phosphate aldolase comprises a polypeptide sequence set forth in the even numbered sequences of SEQ ID NOS: 6, 12, 42, 104, or and 140-478. 4. The engineered deoxyribose-phosphate aldolase of claim 1 , wherein said engineered deoxyribose-phosphate aldolase is purified. 5. A composition comprising at least one engineered deoxyribose-phosphate aldolase provided in claim 1 .
Assignees
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Classifications
Deoxyribose-phosphate aldolase (4.1.2.4) · CPC title
having a known sequence of two or more amino acids, e.g. glutathione · CPC title
- C12N9/88Primary
Lyases (4.) · CPC title
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression · CPC title
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- What does patent US11274286B2 cover?
- The present invention provides engineered deoxyribose-phosphate aldolase polypeptides useful under industrial process conditions for the production of pharmaceutical and fine chemical compounds.
- Who is the assignee on this patent?
- Codexis Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N9/88. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Mar 15 2022 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 2 related publications on this page (citations in our corpus or others sharing the same primary CPC).