Manipulation of microfluidic droplets

Patent metadata

Field	Value
Publication number	US-11268887-B2
Application number	US-202016733137-A
Country	US
Kind code	B2
Filing date	Jan 2, 2020
Priority date	Mar 23, 2009
Publication date	Mar 8, 2022
Grant date	Mar 8, 2022

Abstract

The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

First claim

What is claimed is:

1. A system for controlling droplet flow rate, the system comprising:
a microfluidic device comprising a microfluidic channel and a merge zone, the microfluidic channel comprising a first stream of droplets and a second stream of droplets;
a sensor configured to detect flow rates of the first and second streams of droplets in the microfluidic channel; and
a controller operably connected to the sensor, the controller configured to adjust the flow rates relative to each other to cause the first stream of droplets to intercalate with the second stream of droplet in the merge zone.

2. The system of claim 1 , wherein the controller is configured to adjust the flow rates by adjusting a pressure of one or more fluids.

3. The system of claim 1 , wherein the first and second streams of droplets comprise droplets differing in size, color, refractive index, or extinction coefficient.

4. The system of claim 1 , wherein the first and second streams of droplets contain different biological, biochemical, or chemical entities.

5. The system of claim 1 , wherein a frequency ratio of the first stream of droplets to the second stream of droplets is selected from the group consisting of 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, and 1:10.

6. The system of claim 5 , wherein the frequency ratio of the first stream of droplets to the second stream of droplets is 1:1.

7. The system of claim 1 , wherein droplets of the first stream have a maximum cross-sectional dimension of less than about 100 microns.

8. The system of claim 1 , wherein droplets of the second stream have a maximum cross-sectional dimension of less than about 100 microns.

9. The system of claim 1 , wherein the controller adjusts the flow rates of one or both streams of droplets to maintain an equal flow rate.

10. The system of claim 1 , further comprising a drive infusion system configured to drive the streams of droplets through the microfluidic channel, the drive infusion system selected from the group consisting of a pump, a pressure head, and an electrokinetic drive.

11. The system of claim 10 , wherein the drive infusion system is in communication with the controller.

Assignees

• Bio Rad Laboratories

Inventors

• Miller Benjamin
• Hutchison Brian
• Wilson Andrew
• Larson Jonathan William
• Zhong Qun
• Yurkovetsky Yevgeny
• Link Darren Roy
• Weary Mark

Classifications

• B01L3/0241 Primary

  Drop counters; Drop formers · CPC title

• B01L2400/0478

  pistons · CPC title

• B01L2400/0442

  thermal energy, e.g. vaporisation, bubble jet · CPC title

• B01L3/50273

  characterised by the means or forces applied to move the fluids · CPC title

• Y10T137/8593

  Systems · CPC title