Hybrid materials for biochemical applications

Patent metadata

Field	Value
Publication number	US-11262353-B1
Application number	US-202016946033-A
Country	US
Kind code	B1
Filing date	Jun 3, 2020
Priority date	Jun 3, 2019
Publication date	Mar 1, 2022
Grant date	Mar 1, 2022

Abstract

Materials and methods for the design of hybrid materials comprising a conducting matrix, organic modifiers/linkers and modifying molecules.

First claim

What is claimed is:

1. A hybrid material comprising:
a modified carbon support presenting internal molecular sites available for further attachment chemistry, wherein the internal molecular sites are not limited to the edges of the carbon structure; and
one or more organic or inorganic entities attached to the internal molecular sites.

2. The hybrid material of claim 1 wherein the entity is a functional surface tag.

3. The hybrid material of claim 1 wherein the entity is a biotin or epitope tag.

4. The hybrid material of claim 3 wherein the epitope tag is selected from the group consisting of His and HA tags.

5. The hybrid material of claim 1 wherein the modified carbon is selected from the group consisting of graphene, graphite, fullerenes, and sp2-bonded carbon.

6. The hybrid material of claim 1 wherein the modified carbon is graphite.

7. The hybrid material of claim 1 wherein the modified carbon is graphene.

8. The hybrid material of claim 1 wherein the entity is selected from the group consisting of peptides, proteins, lipids, nucleic acids, antibodies and polymers.

9. A biocompatible electrode comprising the hybrid material of claim 1 .

10. A method for forming a hybrid material comprising:
modifying carbon to produce reactive internal molecular sites that are presented on the surface of the carbon and not limited to the edges of the carbon structure; and
attaching an organic or inorganic entity to the reactive internal molecular sites.

11. The method of claim 10 wherein the reactive internal molecular sites are nitrile groups.

12. The method of claim 10 wherein the step of modifying carbon comprises:
exposing the carbon to tetracyanoethylene oxide (TCNEO) to produce TCNEO-modified carbon; and
reducing the TCNEO-modified carbon.

13. The method of claim 10 wherein the step of attaching an entity comprises solid phase synthesis of the organic or inorganic entity on the modified carbon.

14. The method of claim 10 wherein the step of attaching an entity comprises using covalent modification to attach the entity.

15. The method of claim 10 wherein the entity is a functional surface tag.

16. The method of claim 10 wherein the entity is a biotin or epitope tag.

17. The method of claim 16 wherein the epitope tag is selected from the group consisting of His and HA tags.

18. The method of claim 10 wherein the carbon is selected from the group consisting of graphene, graphite, fullerenes, and sp2-bonded carbon.

19. The method of claim 10 wherein the modified carbon is graphene or graphite.

20. The method of claim 1 wherein the entity is selected from the group consisting of peptides, proteins, lipids, nucleic acids, antibodies and polymers.

Assignees

• Kalugin Nikolai
• Serov Alexey
• Candelaria Lindsay
• Kalugin Peter
• Unm Rainforest Innovations

Inventors

• Kalugin Nikolai
• Serov Alexey
• Candelaria Lindsay
• Kalugin Peter

Classifications

• G01N33/5438

  Electrodes · CPC title

• G01N27/3275

  Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction · CPC title

• G01N33/551

  the carrier being inorganic · CPC title

• G01N33/54353 Primary

  with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title

• C07K1/042

  characterised by the nature of the carrier · CPC title