Method and device for concentrating molecules or objects dissolved in solution

Patent metadata

Field	Value
Publication number	US-11262333-B2
Application number	US-201515500508-A
Country	US
Kind code	B2
Filing date	Aug 3, 2015
Priority date	Aug 1, 2014
Publication date	Mar 1, 2022
Grant date	Mar 1, 2022

Abstract

A method for concentrating electrically charged objects in a non-Newtonian liquid medium comprises: feeding a sample containing electrically charged objects into a channel having a flow axis, a first transverse cross-section orthogonal to the flow axis, and at least one second transverse cross-section orthogonal to the flow axis, one dimension of the second cross-section being less than the corresponding dimension of the first cross-section; and applying a hydrodynamic flow in a direction of the channel together with the application, in the opposite direction, of an electric field in the channel, thus making it possible to move the electrically charged objects in the channel along the flow axis from the first cross-section to the second cross-section, stop the objects, and concentrate the objects in at least one area upstream from the second transverse cross-section.

First claim

The invention claimed is:

1. A process for concentrating electrically charged objects in a non-newtonian liquid medium, comprising:
the introduction of a sample containing electrically charged objects into a channel exhibiting a flow axis, a first transverse section orthogonal to the flow axis and at least one second transverse section orthogonal to the flow axis, the minimum dimension of said second transverse section being less than the corresponding dimension of said first transverse section, wherein temperatures within the first transverse section and within the second transverse section are at ambient temperature, the electrically charged objects being intended to migrate along the flow axis from a first reservoir to a second reservoir;
the application of a hydrodynamic flow, in the first and second reservoirs by means of pressure control means of the first reservoir and of the second reservoir, in a direction of said channel in conjunction with the application, in the reverse direction, of a non-alternating electric field in said channel by means of electrodes in the first and second reservoirs, making it possible to displace the electrically charged objects in the channel along the flow axis of the first transverse section toward the second section and to halt them and to concentrate them in at least one zone upstream of said second transverse section.

2. The process as claimed in claim 1 , wherein the liquid medium comprises uncharged polymers.

3. The process as claimed in claim 2 , wherein the uncharged polymers are chosen from polyvinylpyrrolidone, poly(ethylene glycol), polyacrylamide and their mixtures.

4. The process as claimed in claim 3 , wherein the uncharged polymers are present in a concentration by weight of 0.1 to 10%, preferably of 0.5 to 5% and more particularly preferably of 1 to 4%.

5. The process as claimed in claim 1 , wherein:
the applied electric field has a value of 10 V/m to 10 000 V/m, preferably of 100 V/m to 5000 V/m and more particularly preferably of 200 V/m to 1000 V/m; and/or
the hydrodynamic flow is characterized by a mean velocity of 1 to 10 000 μm/s, preferably of 5 to 5000 μm/s and more particularly preferably of 10 to 1000 μm/s.

6. The process as claimed in claim 1 , wherein:
the introduction of the electrically charged objects is carried out in an introduction zone of the channel and the displacement of the electrically charged objects is carried out from the introduction zone toward a detection zone of the channel, the process additionally comprising:
the detection of the electrically charged objects arriving in the detection zone.

7. The process as claimed in claim 1 , wherein said electrically charged objects are chosen from the group consisting of: single- or double-stranded DNA or RNA molecules comprising at least 20 bases or base pairs; peptides, polypeptides or proteins comprising at least 100 amino acid units; polymeric carbohydrates or other polymers; karyotic or prokaryotic cells; nanoobjects or nanoparticles.

8. A device for concentrating electrically charged objects in a liquid medium, comprising:
a channel exhibiting a flow axis, the channel being filled with a non-newtonian liquid medium, the electrically charged objects being intended to migrate along the flow axis from a first reservoir to a second reservoir;
means for application of a hydrodynamic flow in the first and second reservoirs by means of pressure control means of the first reservoir and of the second reservoir; and
means for application of a non-alternating electric field in the channel by means of electrodes in the first and second reservoirs,
wherein said channel exhibits a first transverse section orthogonal to the flow axis and at least one second transverse section orthogonal to the flow axis, the minimum dimension of said second transverse section being lower than the corresponding dimension of said first transverse section, wherein a first temperature within the first transverse section and a second temperature within the second transverse section are at ambient temperature.

9. The device as claimed in claim 8 , wherein said channel has the shape of a hollow tube of rectangular section exhibiting a lower wall, an upper wall and two side walls, said side walls locally forming at least one constriction.

10. The device as claimed in claim 8 , wherein said second section transverse exhibits a minimum dimension lower by at least 10% or by 20% or by 50% or by 95% than the corresponding dimension of said first transverse section.

11. The device as claimed in claim 9 , wherein said side walls of said hollow tube each exhibit a portion forming an angle of between 10° and 65° with said flow axis.

12. The device as claimed in claim 8 , wherein said channel is a lumen of a capillary exhibiting at least one constriction.

13. The device as claimed in claim 12 , wherein said capillary exhibits a square or circular section.

14. The device as claimed in claim 12 , wherein said capillary exhibits a portion forming an angle of between 10° and 65° with said flow axis.

15. The process as claimed in claim 1 , wherein said channel has the shape of a hollow tube of rectangular section exhibiting a lower wall, an upper wall and two side walls, said side walls locally forming at least one constriction, and said side walls of said hollow tube each exhibit a portion forming an angle of between 10° and 65° with said flow axis.

16. The process as claimed in claim 1 , wherein said channel has the shape of a hollow tube of rectangular section exhibiting a lower wall, an upper wall and two side walls, said side walls locally forming at least one constriction, said channel is a lumen of a capillary exhibiting at least one constriction, and said capillary exhibits a portion forming an angle of between 10° and 65° with said flow axis.

Assignees

• Centre Nat Rech Scient

Inventors

• Bancaud Aurélien
• Ranchon Hubert
• Leichle Thierry
• Teerapanich Pattamon

Classifications

• G01N2030/004

  characterised by opposing force · CPC title

• G01N30/0005 Primary

  Field flow fractionation · CPC title

• B01L3/502753

  characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation · CPC title

• C12Q1/6806

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• B01L2300/0816

  Cards, e.g. flat sample carriers usually with flow in two horizontal directions · CPC title