Composition for substrate surface modification and method using the same

Patent metadata

Field	Value
Publication number	US-11261204-B2
Application number	US-202017011470-A
Country	US
Kind code	B2
Filing date	Sep 3, 2020
Priority date	Aug 7, 2018
Publication date	Mar 1, 2022
Grant date	Mar 1, 2022

Abstract

The present invention provides a composition for substrate surface modification and a method using the same, and the composition for substrate surface modification is composed of a compound of the general formula structure shown in formula 1:

formula 1, wherein n 1 is an integer of 1 to 6, and R is a zwitterionic group. The composition for substrate surface modification uses water as a medium to perform modifying reaction over a substrate surface, and at the same time has biological modification characteristics, and abilities of immobilizing biomolecules and anti-biofouling.

First claim

What is claimed is:

1. A method for modifying a substrate surface, comprising:
preparing a surface modification solution, comprising:
providing a 2-(dimethylamino) ethyl trimethoxy silane as a reaction initiator;
reacting the reaction initiator with a 4-methyloxetan-2-one to obtain an intermediate product;
purifying the intermediate product to obtain a purified intermediate product;
adding a triethanol amine and a toluene to react with the purified intermediate product to obtain a final reactant;
adding a dimethyl sulfoxide to the final reactant to prepare a standard solution; and
diluting the standard solution to form the surface modification solution;
providing a substrate to be surface-modified; and
coating the surface modification solution on a surface of the substrate to be surface-modified for reaction to modify the surface of the substrate.

2. The method for modifying the substrate surface as claimed in claim 1 , wherein the reaction initiator is reacted with the 4-methyloxetan-2-one in an acetonitrile solution.

3. The method for modifying the substrate surface as claimed in claim 1 , wherein the step of diluting the standard solution comprises using dichloromethane, ethanol or pure water to dilute.

4. The method for modifying the substrate surface as claimed in claim 1 , wherein the substrate is a metal, a polymer or a glass.

5. The method for modifying the substrate surface as claimed in claim 1 , wherein after coating the surface modification solution on the surface of the substrate, the method further comprises steps of:
activating the surface of the surface-modified substrate using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxy succinimide (NHS);
adding a modifying biomolecule to the surface of the substrate for reaction; and
performing a deactivating reaction by adding a buffer solution to the surface of the substrate to obtain the surface-modified substrate by the biomolecule.

Assignees

• Univ Nat Central

Inventors

• Huang Chen-Han
• Chen Wen-Hao

Classifications

• C12N11/02

  Enzymes or microbial cells immobilised on or in an organic carrier · CPC title

• C07K17/06

  attached to the carrier via a bridging agent · CPC title

• C07F7/10 Primary

  containing nitrogen {having a Si-N linkage} · CPC title

• C07K17/08

  the carrier being a synthetic polymer · CPC title

• C12N11/08

  the carrier being a synthetic polymer · CPC title