Synthesis device

Patent metadata

Field	Value
Publication number	US-11260361-B2
Application number	US-201916411811-A
Country	US
Kind code	B2
Filing date	May 14, 2019
Priority date	May 17, 2018
Publication date	Mar 1, 2022
Grant date	Mar 1, 2022

Abstract

A device for synthesis of macromolecules is disclosed. In one aspect, the device comprises an ion-releaser having a synthesis surface comprising an array of synthesis locations arranged for synthesis of the macromolecules. The ion-releaser also includes an ion-source electrode, which is arranged to contain releasable ions and is arranged to be in contact with each of the synthesis locations of the synthesis surface, thereby release ions to the synthesis locations. The ion-releaser further comprises activating electrodes, which are arranged to be in contact with the ion-source electrode, wherein each one of the activating electrodes is arranged in association with one of the synthesis locations via the ion-source electrode. The ion-releaser is arranged to release at least a portion of the releasable ions from the ion-source electrode to one of the synthesis locations, by activation of the activating electrode associated with the synthesis location.

First claim

What is claimed is:

1. A device for synthesis of macromolecules, the device comprising:
an ion-releaser having a synthesis surface comprising an array of synthesis locations arranged for synthesis of the macromolecules, wherein
the ion-releaser comprises:
an ion-source electrode being an ion intercalation electrode and thereby arranged to comprise releasable ions, and arranged to be in contact with each of the synthesis locations of the synthesis surface, thereby releasing ions to the synthesis locations; and
activating electrodes arranged to be in contact with the ion-source electrode, wherein each one of the activating electrodes is arranged in association with one of the synthesis locations, respectively, via the ion-source electrode,
wherein the ion-releaser is arranged to release at least a portion of the releasable ions from the ion-source electrode to one of the synthesis locations, by activation of the activating electrode associated with the one of the synthesis locations.

2. The device according to claim 1 , wherein the ion-source electrode is an insertion electrode.

3. The device according to claim 2 , wherein the ion-source electrode is an ion intercalation electrode.

4. The device according to claim 2 , further comprising a plurality of ion-source electrodes, wherein each one of the plurality of the ion-source electrodes is arranged in connection with one of the synthesis locations, respectively.

5. The device according to claim 2 , wherein the synthesis locations are arranged to be in contact with reactants, reaction products, and reaction media.

6. The device according to claim 2 , wherein the synthesis locations comprise porous oxides configured to support the synthesis of the macromolecules.

7. The device according to claim 1 , wherein the ion intercalation electrode is formed of a material comprising amorphous TiO 2 , amorphous chlorine doped TiO 2 , nanoporous silica, or nanoporous titania.

8. The device according to claim 1 , further comprising a plurality of ion-source electrodes, wherein each one of the plurality of the ion-source electrodes is arranged in connection with one of the synthesis locations, respectively.

9. The device according to claim 1 , wherein the synthesis locations are arranged to be in contact with reactants, reaction products, and reaction media.

10. The device according to claim 1 , wherein the synthesis locations comprise porous oxides configured to support the synthesis of the macromolecules.

11. The device according to claim 1 , further comprising a counter electrode arranged at a distance from the synthesis surface,
wherein activation of the activating electrodes associated with the synthesis locations is realized by provision of voltage and/or current between the activating electrodes and the counter electrode.

12. The device according to claim 11 , further comprising a control unit arranged to control activation of the activating electrodes.

13. The device according to claim 12 , wherein the counter electrode and the ion-source electrode are made of the same material.

14. The device according to claim 11 , further comprising:
a synthesis plate having a first side and a second side and an array of through holes, one through hole for each of the synthesis locations, the through holes extending from the first side to the second side of the synthesis plate,
wherein the first side of the synthesis plate is arranged to be in contact with the synthesis surface of the ion-releaser, each of the through holes being associated with one of the synthesis locations, respectively, the synthesis surface of the ion-releaser forming a bottom portion of each of the through holes, thereby defining a synthesis cavity in each of the through holes comprising a synthesis location.

15. The device according to claim 14 , wherein the counter electrode is arranged to be in contact with the second side of the synthesis plate, and wherein the counter electrode comprises openings in connection with each of the synthesis cavities, thereby allowing transport of matter into or out of each of the synthesis cavities.

16. The device according to claim 11 , wherein the counter electrode and the ion-source electrode are made of the same material.

17. The device according to claim 1 , wherein the releasable ions are ions acting as reactants in the synthesis of the macromolecules, or ions providing catalytic effects on the synthesis of the macromolecules.

18. The device according to claim 1 , wherein the releasable ions are selected from protons, Li + , Na + , Al3 + , Ca2 + , Cu + and Mg2 + , and combinations thereof.

19. A method of synthesis of macromolecules, the method comprising:
providing reactants for the synthesis on at least one of the synthesis locations of the synthesis device of claim 1 ;
releasing at least a portion of the releasable ions from the ion-source electrode to the at least one of the synthesis locations; and
synthesizing the macromolecules from the reactants in the presence of the released ions, on the at least one of the synthesis locations.

Assignees

• Imec Vzw

Inventors

• Vereecken Philippe
• Put Brecht
• Stakenborg Tim
• Furnemont Arnaud
• Di Piazza Luca

Classifications

• C25B3/29

  Coupling reactions · CPC title

• B01J2219/00317

  Microwell devices, i.e. having large numbers of wells · CPC title

• B01J19/0046 Primary

  Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays (synthesis methods per se C40B50/00) · CPC title

• C07H21/00

  Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids · CPC title

• B01J2219/00722

  Nucleotides · CPC title