Modification of plant lipids containing PUFAs

Patent metadata

Field	Value
Publication number	US-11260095-B2
Application number	US-201515526162-A
Country	US
Kind code	B2
Filing date	Nov 13, 2015
Priority date	Nov 14, 2014
Publication date	Mar 1, 2022
Grant date	Mar 1, 2022

Abstract

The present invention generally is concerned with the modification of plant lipids containing PUFAs. In this context, the invention is particularly concerned with plants and plant materials for such modifications, wherein the plants preferably are oilseed plants. Regarding plant parts, the invention is particularly concerned with seeds of such plants and preferably seeds of oilseed plants. The invention is also concerned with plant positions obtainable or obtained by the modification method of the invention, and with full stuff of feedstuff comprising such liquid compositions.

First claim

The invention claimed is:

1. Brassica napus plant or part thereof, comprising seeds that comprise lipids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and optionally arachidonic acid (ARA), wherein
a) the content of EPA is at least 5% higher than of ARA, and/or
b) the sum of contents of EPA+DHA is at least 7% higher than ARA and/or
c) the content of ARA is less than 4% and the content of EPA is more than 7% and the content of DHA is more than 2%, and
wherein the Brassica napus plant or part thereof comprises a Delta-5 elongase gene under the control of a FAE promoter.

2. Method of altering plant seed lipids composition, comprising the step of growing a Brassica napus plant according to claim 1 to produce seeds that comprise lipids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and arachidonic acid (ARA), wherein the step of growing and lipids production is continued until the content of ARA in the lipids of the seeds has decreased.

3. Method of producing a Brassica napus plant seed lipid composition, comprising the steps of
a) growing Brassica napus plants according to claim 1 ,
b) harvesting the plants or a part thereof when the lipids content of ARA in the lipids of the seeds has decreased.

4. Method according to claim 2 , wherein ARA in the lipids of the seeds decreases by at least 0.5 wt.-%.

5. The Brassica napus plant or part thereof of claim 1 , wherein the content of EPA is at least 5% higher than of ARA in the lipids of the seeds.

6. The Brassica napus plant or part thereof of claim 1 , wherein the sum of contents of EPA+DHA is at least 7% higher than ARA in the lipids of the seeds.

7. The Brassica napus plant or part thereof of claim 1 , wherein the content of ARA in the lipids of the seeds is less than 4% and the content of EPA in the lipids of the seeds is more than 7% and the content of DHA in the lipids of the seeds is more than 2%.

8. The Brassica napus plant or part thereof of claim 1 , wherein expression of the Delta-5 elongase gene is maintained or increased in late stage seed development.

9. Brassica napus plant comprising seeds that comprise lipids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and arachidonic acid (ARA), wherein, when the Brassica napus plant or part thereof is grown, the content of ARA in the lipids of the seeds decreases at least 0.5% by weight of total lipids and the content of EPA and/or DHA in the lipids of the seeds increases, wherein the seeds comprise a Delta-5 elongase gene under the control of a FAE promoter.

10. Brassica napus plant according to claim 9 , wherein the content of EPA in the lipids of the seeds increases at least 1.0% by weight of total lipids during late stage seed development.

11. The Brassica napus plant of claim 9 , wherein, when the Brassica napus plant or part thereof is grown, the content of ARA in the lipids of the seeds decreases at least 0.5% by weight of total lipids and the content of EPA and/or DHA in the lipids of the seeds increases during late stage seed development.

Assignees

• Basf Plant Science Co Gmbh

Inventors

• Senger Toralf
• Andre Carl

Classifications

• C12N15/8247 Primary

  involving modified lipid metabolism, e.g. seed oil composition · CPC title

• A61K36/31 Primary

  Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi · CPC title

• C12N9/93

  Ligases (6) · CPC title

• A23K20/158 Primary

  Fatty acids; Fats; Products containing oils or fats · CPC title

• C12N9/0071

  acting on paired donors with incorporation of molecular oxygen (1.14) · CPC title