Plants containing beneficial endophytes

Patent metadata

Field	Value
Publication number	US-11254908-B2
Application number	US-201916378506-A
Country	US
Kind code	B2
Filing date	Apr 8, 2019
Priority date	Dec 24, 2013
Publication date	Feb 22, 2022
Grant date	Feb 22, 2022

Abstract

This application relates to methods and materials for providing a benefit to a seed, seedling or plant by producing seeds that are internally colonized with endophytes. Beneficial endophytes with particular characteristics are provided. These may be used in the methods described to provide a benefit to a seed, seedling or plant.

First claim

The invention claimed is:

1. A method for generating an agricultural seed internally colonized with a bacterial endophyte of the genus Paenibacillus , the method comprising:
a) contacting at least one flower of a plurality of cereal agricultural plants with a synthetic formulation comprising a purified bacterial population, the bacterial population comprising the bacterial endophyte of the genus Paenibacillus in an amount effective to internally colonize at least one seed made by the flower, wherein the bacterial endophyte of the genus Paenibacillus comprises a 16S nucleic acid sequence comprising SEQ ID NO: 961 and exhibits the ability to produce auxin or acetoin in the internally colonized seed or in a seedling or a cereal agricultural plant grown from the internally colonized seed and wherein the contacting results in a concentration of at least 10 CFU of the bacterial endophyte inside the tissue of the at least one internally colonized seed.

2. The method of claim 1 , wherein the flower is a male flower.

3. The method of claim 1 , wherein the flower is a female flower.

4. The method of claim 1 , further comprising: b) packaging the contacted seeds in a container.

5. The method of claim 1 , wherein the formulation further comprises a stabilizer, a fungicide, a preservative, or a combination thereof.

6. The method of claim 1 , wherein the at least one internally colonized seed is shelf stable at 25 degrees C. for at least 7 days.

7. The method of claim 1 , wherein the at least one internally colonized seed is shelf-stable at 25 degrees C. for at least 6 months.

8. The method of claim 1 , wherein the contacting comprises spraying, immersing, or dusting the at least one flower with the synthetic formulation.

9. The method of claim 1 , wherein the purified bacterial population comprises a plurality of bacterial endophyte entities.

10. The method of claim 1 , wherein the purified bacterial population comprises a plurality of taxonomically diverse bacterial endophyte entities.

11. The method of claim 1 , wherein the cereal agricultural plant is a wheat plant.

12. The method of claim 1 , wherein the cereal agricultural plant is a barley plant.

Assignees

• Indigo Ag Inc
• Ait Austrian Inst Tech Gmbh

Inventors

• Mitter Birgit
• Naveed Muhammad
• Berninger Teresa
• Compant Stephane
• Sessitsch Angela
• Von Maltzahn Geoffrey
• Flavell Richard Bailey
• Toledo Gerardo V
• Djonovic Slavica
• Marquez Luis Miguel
• Johnston David Morris
• Millet Yves Alain
• Lyford Jeffrey
• Leff Jonathan W
• Samayoa Phillip
• Sadowski Craig

Classifications

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• A01N63/20

  Bacteria; Substances produced thereby or obtained therefrom · CPC title

• G01N33/0098

  Plants or trees (wood G01N33/46) · CPC title

• A01N25/00

  Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application {, e.g. seed treatment or sequential application}; Substances for reducing the noxious effect of the active ingredients to organisms other than pests · CPC title