Cell culture vessel

Patent metadata

Field	Value
Publication number	US-11248201-B2
Application number	US-201816630019-A
Country	US
Kind code	B2
Filing date	Jul 13, 2018
Priority date	Jul 13, 2017
Publication date	Feb 15, 2022
Grant date	Feb 15, 2022

Abstract

A cell culture vessel including an accommodating part which is an inner space accommodating a cell culture support therein, a fixing member configured to fix the cell culture support to a lower surface of the accommodating part, wherein the fixing member includes a first adhesive layer attached to the lower surface of the accommodating part, a second adhesive layer attached to a lower surface of the cell culture support, and a support film interposed between the first adhesive layer and the second adhesive layer and configured to perform a support function, and an adhesive force between the second adhesive layer and the cell culture support is higher than an adhesive force between the first adhesive layer and the lower surface of the accommodating part.

First claim

The invention claimed is:

1. A cell culture vessel including an accommodating part which is an inner space accommodating a cell culture support therein, the cell culture vessel comprising:
a fixing member configured to fix the cell culture support which is a nanofiber web formed of nanofiber to a lower surface of the accommodating part,
wherein the fixing member includes a first adhesive layer attached to the lower surface of the accommodating part, a second adhesive layer attached to a lower surface of the cell culture support, and a support film interposed between the first adhesive layer and the second adhesive layer and configured to perform a support function, and
an adhesive force between the second adhesive layer and the cell culture support is higher than an adhesive force between the first adhesive layer and the lower surface of the accommodating part,
wherein the first adhesive layer has a thickness in a range of 7 to 55 μm,
wherein the second adhesive layer has a thickness in a range of 3 to 25 μm,
wherein the adhesive force between the second adhesive layer and the cell culture support is in a range of 7 to 60 gf/inch, and
wherein an adhesive force ratio of the adhesive force between the first adhesive layer and the lower surface of the accommodating part to the adhesive force between the second adhesive layer and the cell culture support is 1:1.5 to 1:3.

2. The cell culture vessel of claim 1 , wherein the adhesive force between the first adhesive layer and the lower surface of the accommodating part is in a range of 3 to 30 gf/inch.

3. The cell culture vessel of claim 1 , wherein the first adhesive layer and the second adhesive layer each independently include one or more selected from the group consisting of a silicone-based adhesive and a urethane-based adhesive.

4. The cell culture vessel of claim 1 , wherein the support film has a thickness in a range of 30 to 220 μm.

5. The cell culture vessel of claim 1 , wherein the support film includes one or more selected from the group consisting of polyethylene, polypropylene, polyimide, cross-linked polypropylene, nylon, polyurethane-based resin, acetate, polybenzimidazole, polyimideamide, polyetherimide, polyphenylene sulfide (PPS), polyethylene terephthalate (PET), polytrimethylene terephthalate (PTT), polybutylene terephthalate (PBT), polyvinylidene fluoride (PVDF), polytetrafluoroethylene (PTFE), polychlorotrifluoroethylene (PCTFE), and polyethylene tetrafluoroethylene (ETFE).

6. The cell culture vessel of claim 1 , wherein the nanofiber web includes a cell culture enhancing substance.

7. The cell culture vessel of claim 6 , wherein the cell culture enhancing substance includes a bioactive component that induces any one or more of adhesion, migration, growth, proliferation, and differentiation of cells.

Assignees

• Amolifescience Co Ltd

Inventors

• Jang Seon Ho
• Koo Song Hee
• Seo In Yong
• Lee Seoung Hoon
• Kim Chan

Classifications

• C12M23/46 Primary

  Means for fastening · CPC title

• C12M25/02

  Membranes; Filters (filters or filtration in general B01D24/00-B01D41/00) · CPC title

• C12M23/12 Primary

  Well or multiwell plates (C12M25/04 takes precedence) · CPC title

• C12M25/14 Primary

  Scaffolds; Matrices (in general C12N5/0068) · CPC title

• C12M23/20

  Material Coatings (immunocoatings C12M25/00) · CPC title