Load lock chamber assemblies for sample analysis systems and related mass spectrometer systems and methods

Patent metadata

Field	Value
Publication number	US-11244819-B2
Application number	US-202016950992-A
Country	US
Kind code	B2
Filing date	Nov 18, 2020
Priority date	Feb 13, 2018
Publication date	Feb 8, 2022
Grant date	Feb 8, 2022

Abstract

Load lock assemblies for a sample analysis system, such as a mass spectrometry system, include a load lock chamber having longitudinally opposing first and second end portions and a through channel, a door coupled to the second end portion, and a seal assembly coupled to the first end portion. The seal assembly includes a rigid seal housing member coupled to a housing of the load lock chamber. The rigid seal housing member includes a port forming part of the first end portion of the through channel of the load lock chamber. The rigid seal housing member can include a self-centering cartridge and/or a flexure member coupled to the housing of the load lock chamber. Where used, the flexure member has an outer perimeter that extends above and below the rigid seal housing member and includes an aperture that is aligned with the port of the rigid seal housing member.

First claim

That which is claimed:

1. A method handling a sample for analysis, comprising:
providing a mass spectrometer with a load lock chamber with opposing first and second end portions, the first end portion being in or adjacent an acquisition vacuum chamber and the second end portion is spaced apart from the first end portion and has a door;
inserting a slide holder of an X-Y stage into the load lock chamber from the acquisition vacuum chamber while the door is sealably closed to the second end portion of the load lock chamber;
automatically sealing the first end portion of the load lock chamber from the acquisition vacuum chamber upon engagement of a seal interface of the slide holder with a seal interface of the first end portion of the load lock chamber;
venting the load lock chamber to atmosphere;
opening the door;
inserting a slide from the second end portion of the load lock chamber to engage the slide holder in the load lock chamber;
closing the door to seal the load lock chamber;
evacuating the load lock chamber to a vacuum pressure; and
retracting the slide holder with the slide into the acquisition vacuum chamber while the door is closed and the load lock chamber is under vacuum, wherein the method further comprises
during set-up or installation:
inserting the slide holder of the X-Y stage into the load lock chamber from the acquisition vacuum chamber;
moving a seal assembly cartridge from a nominal position side to side and/or up or down to a self-centered second position to align with the seal interface of the slide holder in response to the inserting; and then
securing the seal assembly cartridge to a fixed position on a housing of the load lock chamber to provide an operative aligned position with the X-Y stage.

2. The method of claim 1 , further comprising analyzing a sample in or on the slide, wherein the sample is analyzed to identify one or more microorganisms in a mass range between about 2000 to about 20,000 Dalton.

3. The method of claim 1 , wherein the second end portion of the load lock chamber is oblong in a lateral dimension with laterally opposing arcuate ends.

4. The method of claim 1 , wherein the load lock chamber has a lateral width that is greater than a height and a volume that is in a range of about 1 cc and about 100 cc.

5. The method of claim 1 , further comprising at least one valve in communication with the load lock chamber, wherein the venting is carried out by opening the at least one valve to atmosphere and the evacuating is carried out, in part, by closing the at least one valve.

6. The method of claim 1 , wherein the closing is carried out by pivoting the door to shut and seal against the second end portion of the load lock chamber.

7. A method handling a sample for analysis, comprising:
providing a mass spectrometer with a load lock chamber with opposing first and second end portions, the first end portion being in or adjacent an acquisition vacuum chamber and the second end portion is spaced apart from the first end portion and has a door;
inserting a slide holder of an X-Y stage into the load lock chamber from the acquisition vacuum chamber while the door is sealably closed to the second end portion of the load lock chamber;
automatically sealing the first end portion of the load lock chamber from the acquisition vacuum chamber upon engagement of a seal interface of the slide holder with a seal interface of the first end portion of the load lock chamber;
venting the load lock chamber to atmosphere;
opening the door;
inserting a slide from the second end portion of the load lock chamber to engage the slide holder in the load lock chamber;
closing the door to seal the load lock chamber;
evacuating the load lock chamber to a vacuum pressure;
retracting the slide holder with the slide into the acquisition vacuum chamber while the door is closed and the load lock chamber is under vacuum; and
flexing a flexure member on the first end portion of the load lock chamber in response to the venting and/or the evacuating, wherein the flexure member comprises an outer perimeter that extends above and below a rigid seal housing member on the first end portion of the load lock chamber, and wherein the flexure member comprises an aperture that is aligned with the load lock chamber.

8. The method of claim 7 , wherein the flexure member comprises a thin sheet, and wherein the outer perimeter of the flexure member comprises a plurality of spaced apart apertures that align with apertures in a flange of the housing of the load lock chamber.

9. The method of claim 7 , wherein the rigid seal housing member comprises an outer facing surface with a channel that extends about port, and wherein the rigid seal housing member holds an O-ring in the channel so that the O-ring projects a distance outside the channel.

10. The method of claim 9 , wherein the channel of the outer facing surface and the O-ring extend about an entire perimeter of the first end portion of the load lock chamber and both the channel and the first end portion of the load lock chamber are oblong in a lateral dimension with opposing laterally spaced apart arcuate sides.

11. A method handling a sample for analysis, comprising:
providing a mass spectrometer with a load lock chamber with opposing first and second end portions, the first end portion being in or adjacent an acquisition vacuum chamber and the second end portion is spaced apart from the first end portion and has a door;
inserting a slide holder of an X-Y stage into the load lock chamber from the acquisition vacuum chamber while the door is sealably closed to the second end portion of the load lock chamber;
automatically sealing the first end portion of the load lock chamber from the acquisition vacuum chamber upon engagement of a seal interface of the slide holder with a seal interface of the first end portion of the load lock chamber;
venting the load lock chamber to atmosphere;
opening the door;
inserting a slide from the second end portion of the load lock chamber to engage the slide holder in the load lock chamber;
closing the door to seal the load lock chamber;
evacuating the load lock chamber to a vacuum pressure; and
retracting the slide holder with the slide into the acquisition vacuum chamber while the door is closed and the load lock chamber is under vacuum,
wherein the closing is carried out by pivoting the door to shut and seal against the second end portion of the load lock chamber, wherein the door is coupled to a motor with a shaft and the shaft is coupled to a first gear that engages a second gear that is coupled to the door whereby the door is decoupled and spaced apart from the second end portion of the load lock chamber with the door in an open position.

12. The method of claim 11 , wherein the second gear has only three sides with one of the three sides having an outer perimeter with gear teeth that engage the first gear and that has an angular extent in a range of about 60-120 degrees.

13. A method handling a sample for analysis, comprising:
providing a mass spectrometer with a seal assembly cartridge and a load lock chamber with opposing first and second end portions, the first end portion being in or adjacent an acquisition vacuum chamber and the second end portion is spaced apart from the first end portion and has a door;
inserting a slide holder of an X-Y stage into the load lock chamber from the acquisition vacuum chamber while the door is sealably closed to the second end portion of the load lock chamber;
automatically sealing the first end portion of the load lock chamber from the acquisition vacuum chamber upon engagement of a seal interface of the

Assignees

• Bio Merieux Inc

Inventors

• Bullock Jared
• Collins Scott
• Talmer Mark

Classifications

• H01J49/0409

  Sample holders or containers (containers for retaining a material to be analyzed, B01L3/50, for DNA, C12Q1/6834, for biological materials, G01N33/543) · CPC title

• H01J49/0031

  Step by step routines describing the use of the apparatus (H01J49/0081 takes precedence) · CPC title

• H01J49/0495 Primary

  Vacuum locks; Valves (valves per se F16K) · CPC title

• G01N2035/00039

  Transport arrangements specific to flat sample substrates, e.g. pusher blade · CPC title

• H01J37/18

  Vacuum locks {; Means for obtaining or maintaining the desired pressure within the vessel} · CPC title