Insect inhibitory toxin family active against hemipteran and/or lepidopteran insects

Patent metadata

Field	Value
Publication number	US-11242540-B2
Application number	US-202015931303-A
Country	US
Kind code	B2
Filing date	May 13, 2020
Priority date	Apr 7, 2011
Publication date	Feb 8, 2022
Grant date	Feb 8, 2022

Abstract

The present invention discloses a genus of insect inhibitory proteins that exhibit properties directed to controlling Lepidopteran and/or Hemipteran crop pests, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.

First claim

What is claimed is:

1. A recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide segment encoding an insect inhibitory protein, wherein said polynucleotide segment comprises at least 80% nucleic acid sequence identity to SEQ ID NO: 27, and further comprises sequences corresponding to motifs M0, M1, M2, M3, M4, and M5, and wherein said insect inhibitory protein exhibits insect inhibitory activity against a Hemipteran or Lepidopteran pest species.

2. The recombinant nucleic acid molecule of claim 1 , wherein said polynucleotide segment comprises a polynucleotide sequence having at least 90% identity to SEQ ID NO: 27.

3. The recombinant nucleic acid molecule of claim 1 , wherein said polynucleotide segment comprises a polynucleotide sequence having at least 95% identity to SEQ ID NO: 27.

4. An insect inhibitory composition comprising the recombinant nucleic acid molecule of claim 1 .

5. The insect inhibitory composition of claim 4 , further comprising at least one other pesticidal agent, wherein said pesticidal agent is structurally different from said insect inhibitory protein, wherein said pesticidal agent is selected from the group consisting of an insect inhibitory protein, an insect inhibitory dsRNA molecule, and an ancillary protein.

6. The insect inhibitory composition of claim 5 , wherein said pesticidal agent is selected from the group consisting of a TIC807 protein, a TIC853 protein, a Cry51Aa1 protein, and a AXMI-171 protein.

7. A method of controlling a Lepidopteran or Hemipteran species pest, said method comprising contacting said pest with an insect inhibitory amount of the insect inhibitory protein encoded by the recombinant nucleic acid of claim 1 .

8. The method of claim 7 , wherein said Lepidopteran or Hemipteran species pest is in a crop field.

9. A host cell comprising the recombinant nucleic acid molecule of claim 1 , wherein the host cell is selected from the group consisting of a bacterial cell and a plant cell.

10. The host cell of claim 9 , wherein said bacterial host cell is from a genus of bacteria selected from the group consisting of: Bacillus, Escherichia, Salmonella, Agrobacterium, Pseudomonas , and Rhizobium.

11. The host cell of claim 9 , wherein said plant host cell is a cell of a plant selected from the group consisting of alfalfa, banana, barley, bean, broccoli, cabbage, brassica , carrot, cassava, castor, cauliflower, celery, chickpea, Chinese cabbage, coconut, coffee, corn, clover, cotton, cucumber, Douglas fir, eggplant, eucalyptus , flax, garlic, grape, hops, leek, lettuce, Loblolly pine, millet, melon, oat, olive, onion, palm, pasture grass, pea, peanut, pepper, pigeonpea, potato, poplar, pumpkin, Radiata pine, radish, rapeseed, rice, rye, safflower, sorghum, Southern pine, soybean, spinach, squash, strawberry, sugar beet, sugarcane, sunflower, sweet corn, sweet gum, sweet potato, switchgrass, tea, tobacco, tomato, triticale, turf grass, watermelon, and wheat.

12. The host cell of claim 9 , wherein said plant host cell is from a plant part selected from the group consisting of a seed, a boll, a leaf, a flower, a stem, and a root.

13. The host cell of claim 9 , wherein said host cell further comprises an herbicide tolerance marker.

14. The recombinant nucleic acid molecule of claim 1 , wherein the recombinant nucleic acid molecule:
(a) further comprises a sequence that functions to express said protein in a plant; or
(b) is expressed in a plant cell to produce an insect inhibitory amount of said protein.

15. A plant comprising a recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide segment encoding an insect inhibitory protein, wherein said polynucleotide segment comprises at least 80% nucleic acid sequence identity to SEQ ID NO:27, and further comprises sequences corresponding to motifs M0, M1, M2, M3, M4, and M5, and wherein said insect inhibitory protein exhibits insect inhibitory activity against a Hemipteran or Lepidopteran pest species.

16. A seed from the plant of claim 15 , wherein the seed comprises said recombinant nucleic acid molecule.

17. A method of producing seed comprising the recombinant nucleic acid molecule of claim 1 , the method comprising:
(a) planting at least one seed that comprises the recombinant nucleic acid molecule of claim 1 ;
(b) growing at least one plant from said at least one seed;
(c) harvesting seed from said at least one plant, wherein said harvested seed comprises said recombinant nucleic acid molecule.

18. A plant resistant to insect infestation, wherein the cells of the plant comprise an insecticidally effective amount of an insect inhibitory protein wherein said amino acid sequence is encoded by a polynucleotide comprising at least 80% nucleic acid sequence identity to SEQ ID NO: 27, and further comprises sequences corresponding to motifs M0, M1, M2, M3, M4, and M5, and wherein said insect inhibitory protein exhibits insect inhibitory activity against a Hemipteran or Lepidopteran pest species.

19. The recombinant nucleic acid molecule of claim 18 , wherein said polynucleotide segment comprises a polynucleotide sequence having at least 90% identity to SEQ ID NO: 27.

20. The recombinant nucleic acid molecule of claim 18 , wherein said polynucleotide segment comprises a polynucleotide sequence having at least 95% identity to SEQ ID NO: 27.

Assignees

• Monsanto Technology Llc

Inventors

• Bowen David J
• Chay Catherine
• Evdokimov Artem
• Flasinski Stanislaw
• Kesanapalli Uma R
• Schroder Megan N
• Slightom Rachael N
• Tao Nengbing
• Wollacott Andrew M

Classifications

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C07K14/32

  from Bacillus (G) · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title

• A01N65/04

  Pteridophyta [fern allies]; Filicophyta [ferns] · CPC title

• C07K14/00 Primary

  Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof · CPC title