Apparatus for performing contactless optically-induced dielectrophoresis for separation of circulating tumor cells

Patent metadata

Field	Value
Publication number	US-11241699-B2
Application number	US-201916577863-A
Country	US
Kind code	B2
Filing date	Sep 20, 2019
Priority date	Apr 19, 2016
Publication date	Feb 8, 2022
Grant date	Feb 8, 2022

Abstract

A apparatus for performing contactless ODEP for separation of CTCs comprises an ODEP device including a first conductive glass and a second conductive glass, the first conductive glass includes a transverse main channel and a longitudinal micro channel perpendicular to the main channel and joining the main channel at a cell separation zone; the first conductive glass includes a first hole and a second hole aligned with two ends of the main channel respectively, and a third hole aligned with one end of the micro channel that is distal to the cell separation zone; a sample receiving member disposed on and aligned with the first hole; an exhaust discharge member disposed on and aligned with the second hole; a target collection member disposed on and aligned with the third hole; and a controller including an optical projection device and an image fetch device.

First claim

What is claimed is:

1. An apparatus for performing contactless optically-induced dielectrophoresis for separation of circulating tumor cells, the apparatus comprising:
an ODEP device including a first conductive glass and a second conductive glass wherein the first conductive glass is disposed on the second conductive glass; the first conductive glass includes a transverse main channel and a longitudinal micro channel perpendicular to the main channel and joining the main channel at a cell separation zone; and the first conductive glass includes a first hole for receiving a sample and a second hole for discharging an exhaust aligned with two ends of the main channel respectively, and a third hole for collecting a target aligned with one end of the micro channel;
a first electrode channel disposed along an edge of the first conductive glass and corresponding to the main channel, and a second electrode channel perpendicular to the first electrode channel, joining the first electrode channel, and corresponding to the micro channel; and
a controller including an optical projection device and an image fetch device.

2. The apparatus of claim 1 , further comprising a light guide layer formed on a top of the second conductive glass, the light guide layer being between the first conductive glass and the second conductive glass.

3. The apparatus of claim 1 , wherein the ODEP device includes a fluid driver and a signal generation device connected to the fluid driver.

Assignees

• Univ Chang Gung
• Ace Medical Tech Co Ltd

Inventors

• Wu Min-Hsien

Classifications

• G01N33/575

  for cancer · CPC title

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• B03C5/026

  using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC] · CPC title

• G01N33/563

  involving antibody fragments · CPC title

• B01L2400/0454

  radiation pressure, optical tweezers · CPC title