Gene sequencing reaction device, gene sequencing system, and gene sequencing reaction method

Patent metadata

Field	Value
Publication number	US-11241692-B2
Application number	US-201716635347-A
Country	US
Kind code	B2
Filing date	Aug 1, 2017
Priority date	Aug 1, 2017
Publication date	Feb 8, 2022
Grant date	Feb 8, 2022

Abstract

A gene sequencing reaction device, a gene sequencing system and a gene sequencing reaction method. The gene sequencing reaction device includes: a supporting platform; a dipping container disposed on the supporting platform, wherein the dipping container has a dipping reaction area, and the dipping reaction area is configured to hold a chemical reagent for gene sequencing reaction, so as to dip a sequencing chip having a DNA sample loading structure on the surface and having a DNA sample loaded thereon in the chemical reagent to perform a gene sequencing reaction; a temperature control apparatus, configured to control the temperature of the chemical reagent in the dipping reaction area; and a transfer apparatus, configured to insert the sequencing chip into the dipping reaction area or pull out the sequencing chip from the dipping reaction area.

First claim

The invention claimed is:

1. A gene sequencing reaction device, comprising:
a supporting platform;
a dipping container disposed on the supporting platform, wherein the dipping container has a dipping reaction area configured to hold a chemical reagent for gene sequencing reaction, so as to dip a sequencing chip having a DNA sample loading structure on the surface and having a DNA sample loaded thereon in the chemical reagent to perform a gene sequencing reaction;
a temperature control apparatus, configured to control the temperature of the chemical reagent in the dipping reaction area; and
a transfer apparatus, configured to insert the sequencing chip into the dipping reaction area or pull out the sequencing chip from the dipping reaction area;
wherein the temperature control apparatus comprises a temperature control portion and a water bath kettle, wherein the water bath kettle is configured to hold liquid capable of transferring heat, the dipping container is disposed in the water bath kettle, and the temperature control portion is configured to control the temperature of the liquid in the water bath kettle to control the temperature of the chemical reagent in the dipping reaction area.

2. The gene sequencing reaction device according to claim 1 , wherein the gene sequencing reaction device comprises a plurality of dipping containers; and/or, the dipping container comprises a plurality of dipping reaction areas isolated from each other.

3. The gene sequencing reaction device according to claim 1 , comprising:
a chip supplying storage apparatus, configured to contain the sequencing chip on which the gene sequencing reaction is about to be performed; and/or,
a chip unloading storage apparatus, configured to contain the sequencing chip on which the gene sequencing reaction has been completed.

4. The gene sequencing reaction device according to claim 3 , wherein the chip supplying storage apparatus and/or the chip unloading storage apparatus comprises:
a chip storage box, wherein the top of the chip storage box is open and is provided with a slot and a drain hole, the slot is configured to position and contain the sequencing chip, and the drain hole is formed in a bottom wall of the chip storage box to discharge the liquid in the chip storage box; and,
a liquid storage box disposed below the drain hole for receiving the liquid discharged from the drain hole.

5. The gene sequencing reaction device according to claim 1 , comprising a container cover being configured to be capable of being opened and closed and covered on the dipping container to prevent the evaporation of the chemical reagent.

6. The gene sequencing reaction device according to claim 5 , wherein the container cover comprises a plurality of cover bodies, each of the cover bodies is correspondingly disposed with one or more dipping reaction areas to prevent the evaporation of the chemical reagent in the corresponding dipping reaction areas, and at least one of the cover bodies is opened and closed independently relative to other cover bodies.

7. The gene sequencing reaction device according to claim 1 , wherein the transfer apparatus comprises a connecting portion configured to be connected with the sequencing chip and a movement mechanism being in driving connection with the connecting portion to change a working position of the connecting portion.

8. The gene sequencing reaction device according to claim 7 , wherein the plurality of dipping reaction areas are disposed in one or more rows along a lateral direction; the movement mechanism comprises a lateral movement mechanism, a longitudinal movement mechanism and a vertical movement mechanism, the longitudinal movement mechanism is disposed on the supporting platform, the lateral movement mechanism is disposed on the longitudinal movement mechanism, the vertical movement mechanism is disposed on the lateral movement mechanism, the connecting portion is disposed on the vertical movement mechanism, the longitudinal movement mechanism drives the lateral movement mechanism to perform longitudinal movement, the lateral movement mechanism drives the vertical movement mechanism to perform lateral movement, and the vertical movement mechanism drives the connecting portion to perform vertical movement.

9. The gene sequencing reaction device according to claim 1 , comprising a chip holding apparatus, wherein the chip holding apparatus comprises one or more chip mounting positions, and the sequencing chip is mounted on the chip mounting position so as to move the sequencing chip by moving the chip holding apparatus.

10. The gene sequencing reaction device according to claim 9 , wherein the DNA sample loading structures are provided on both sides of the sequencing chip are provided with; and the chip mounting position comprises a chip mounting opening, the sequencing chip is mounted in the chip mounting opening, and the chip mounting opening is a through opening with both open sides.

11. The gene sequencing reaction device according to claim 9 , wherein the chip holding apparatus comprises a chip framework, the chip mounting position is disposed on the chip framework, the surface of the chip framework is a hydrophobic surface, and/or, a lower end of the chip framework is gradually tapered from top to bottom.

12. The gene sequencing reaction device according to claim 9 , wherein the chip holding apparatus comprises a chip framework, one or more chip mounting positions are disposed on the chip framework, and an upper end of the chip framework is provided with a jaw fitting opening; the transfer apparatus comprises a clamping jaw connected with the chip framework and a movement mechanism in driving connection with the clamping jaw to change the working position of the clamping jaw, the clamping jaw comprises a positioning frame and a clamping block, the positioning frame ( 11 ) is connected with the movement mechanism, the clamping block is movably disposed on the positioning frame, the positioning frame is provided with a positioning hole for inserting the upper end of the chip framework, and the clamping jaw is clamped with the jaw fitting opening of the chip framework inserted into the positioning hole so as to clamp the chip framework ( 9 ).

13. The gene sequencing reaction device according to claim 1 , comprising a controller, wherein,
the controller is coupled with the temperature control apparatus to control the temperature of the chemical reagent; and/or,
the controller is coupled with the transfer apparatus to control the dipping time and/or dipping sequence of the sequencing chip in the dipping reaction area.

14. The gene sequencing reaction device according to claim 1 , comprising the sequencing chip, and the surface of the sequencing chip is provided with the DNA sample loading structure.

15. The gene sequencing reaction device according to claim 1 , comprising a protective cover, and the dipping container is disposed in the protective cover.

16. The gene sequencing reaction device according to claim 1 , comprising an air blowing apparatus, configured to blow off the chemical reagent on the surface of the sequencing chip and/or a surface of a chip holding apparatus on which the sequencing chip is mounted.

17. A gene sequencing system, comprising a DNA sample loading device and a gene sequencing reaction device, wherein the gene sequencing reaction device is the gene sequencing reaction device according to claim 1 .

18. A gene sequencing reaction method, comprising:
adding a chemical reagent for gene sequencing reaction into a dipping reaction area o

Assignees

• Mgi Tech Co Ltd

Inventors

• Ma Wei
• Xu Xun
• Wu Jiabo
• Ni Ming
• Wei Dong
• Tang Jiansheng

Classifications

• B01L2300/0819

  Microarrays; Biochips · CPC title

• B01L2300/185

  using a liquid as fluid · CPC title

• C12Q1/6874

  involving nucleic acid arrays, e.g. sequencing by hybridisation · CPC title

• B01L7/02

  Water baths; Sand baths; Air baths · CPC title

• B01L9/52 Primary

  Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips · CPC title