Feed additive composition

Patent metadata

Field	Value
Publication number	US-11235035-B2
Application number	US-201816208829-A
Country	US
Kind code	B2
Filing date	Dec 4, 2018
Priority date	Aug 3, 2012
Publication date	Feb 1, 2022
Grant date	Feb 1, 2022

Abstract

A method for improving the performance of a subject or for improving digestibility of a raw material in a feed (e.g. nutrient digestibility, such as amino acid digestibility), or for improving nitrogen retention, or for improving dietary phosphorus absorption and retention, or for improving the efficacy of the phytase, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating (e.g. improving) the immune response of the subject or for reducing populations of pathogenic bacteria in the gastrointestinal tract of a subject, or for reducing nutrient excretion in manure, which method comprising administering to a subject at least one direct fed microbial in combination with a phytase, wherein the phytase is administered to the subject at a dosage of more than about 1500 FTU/kg feed.

First claim

The invention claimed is:

1. A method for improving the performance of a subject or for improving digestibility of a raw material in a feed, or for improving nitrogen retention, or for improving dietary phosphorus absorption and retention, or for improving the efficacy of the phytase, or for improving the subject's resistance to necrotic enteritis or for improving feed conversion ratio (FCR) or for improving weight gain in a subject or for improving feed efficiency in a subject or for modulating the immune response of the subject or for reducing populations of pathogenic bacteria in the gastrointestinal tract of a subject, or for reducing nutrient excretion in manure, which method comprising administering to a subject at least one direct fed microbial comprising Bacillus amyloliquefaciens in combination with a phytase, wherein the phytase is administered to the subject at a dosage of more than about 1500 FTU/kg feed.

2. A method according to claim 1 wherein the direct fed microbial is an antipathogen direct fed microbial.

3. A method according to claim 1 wherein the direct fed microbial is a viable bacterium.

4. A method according to claim 1 wherein the phytase is administered to the subject at a dosage of more than about 2000 FTU/kg feed.

5. A method according to claim 1 wherein the phytase is administered to the subject at a dosage of more than about 3000 FTU/kg feed.

6. A method according to claim 1 wherein the composition comprises as least three direct fed microbials.

7. A method according to claim 1 wherein the direct fed microbial is a strain which inhibits intestinal establishment of pathogenic microorganisms.

8. A method according to claim 7 wherein the pathogenic microorganisms are Clostridium perfringens and/or E. coli.

9. A method according to claim 1 wherein the direct fed microbial further comprises a bacterium from one or more of the following genera: Bacillus, Enterococcus, Pediococcus, Saccharomyces, Bifidobacterium, Propionibacterium, Lactobacillus, Lactococcus, Aspergillus and combinations thereof.

10. A method according to claim 1 wherein the direct fed microbial further comprises a bacterium from one or more of the following species: Bacillus subtilis, Bacillus licheniformis, Bacillus cereus, Enterococcus faecium, Pediococcus acidilactici, Saccharomyces cerevisiae, Bifidobacterium animalis spp animalis, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus acidophilus, Lactobacillus brevis, Lactobacillus salivarius ssp salivarius, Lactobacillus farciminis, Lactococcus lactis, Lactobacillus casei, Lactobacillus salivarius, Clostridium butyricum, Aspergillus oryzae, Propionibacterium acidipropionici, Propionibacterium jensenii, and combinations thereof.

11. A method according to claim 1 wherein the direct fed microbial further comprises one or more of the following strains: Bacillus subtilis BS18 (NRRL B-50633), Bacillus subtilis BS278 (NRRL 50634), Bacillus subtilis 4-7 d (NRRL B-50505), Bacillus subtilis 3-5 h (NRRL B-50507), Bacillus subtilis AGTP BS3BP5 (NRRL B-50510), Bacillus subtilis BS918 (NRRL B-50508), Bacillus subtilis AGTP BS1013 (NRRL-50509), B. subtilis AGTP 944 (NRRL B-50548), Bacillus subtilis AGTP BS442 (NRRL B-50542), B. subtilis AGTP BS1069 (NRRL B-50544), B. subtilis AGTP BS521 (NRRL B-50545), B. subtilis BS2084 (NRRL B-50013), B. subtilis LSSA01 (NRRL B-50104), B. subtilis B27 (NRRL B-50105), B. subtilis 3A-P4 (PTA-6506), Bacillus subtilis 15A-P4 (PTA-6507), B. subtilis 22C-P1 (PTA-6508), B. subtilis BL21 (NRRL B-50134), Bacillus licheniformis BL21 (NRRL B-50134), Bacillus licheniformis 3-12a (NRRL B-50504), Bacillus licheniformis 4-2a (NRRL B-50506), Bacillus licheniformis 842 (NRRL B-50516), Propionibacterium acidipropionici P261 (NRRL B-50131), Propionibacterium acidipropionici P179 (NRRL B-50133), Propionibacterium acidipropionici P169 (PTA 5271), Propionibacterium acidipropionici P170 (PTA 5272), Propionibacterium jensenii P63 (NRRL B-30979), Propionibacterium jensenii P195 (NRRL B-50132), Lactococcus lactis ID7 (PTA 6103), Lactococcus lactis JD19 (PTA 6104), Lactobacillus acidophilus A2020 (NRRL B-30977), Lactobacillus acidophilus A4000 h (NRRL B-30978), Lactobacillus acidophilus PIBc6 (NRRL B-50103), Lactobacillus brevis LBR 1000 (NRRL B-30982), Lactobacillus casei LC222 (NRRL B-30983), Lactobacillus johnsonii PLCB6 (NRRL B-50518), Lactobacillus salivarius o246i33w (NRRL B-50102), Lactobacillus brevis AJ25 (PTA-6099), Lactobacillus brevis HE17 (PTA-6100), Lactobacillus brevis 1E-1 (PTA-6509), Lactobacillus lactis C115 (PTA-6101), Lactobacillus lactis DJ6 (PTA-6102), Lactobacillus rhamnosus (CNCM 1-3698), Lactobacillus farciminis (CNCM 1-3699), Enterococcus faecium EF141 (EN-1) (NRRL B-30981), Enterococcus faecium 2-1 d (NRRL B-50519), Pediococcus acidilactici PIJe3 (NRRL B-50101), Pediococcus acidilactici o246e42 (NRRL B-50171) and combinations thereof.

12. A method use according to claim 1 wherein the phytase is a 6-phytase or a 3-phytase.

13. A method according to claim 12 wherein the phytase is a 6-phytase.

14. A method according to claim 1 wherein the phytase is an E. coli phytase or a Buttiauxella phytase or a Citrobacter phytase or a Hafnia phytase or an Aspergillus phytase or a Penicillium phytase or a Trichoderma phytase or a Pichia phytase or a Peniophora phytase.

15. A method according to claim 1 wherein the DFM is present at a dosage of 3.75×10 7 CFU/g feed additive composition and 1×10 11 CFU/g feed additive composition.

16. A method according to claim 1 wherein a clinical or subclinical intestinal disease challenge is present in the subject.

17. A method according to claim 16 wherein the clinical or subclinical intestinal disease challenge may be caused by a pathogenic bacteria.

18. A method according to claim 17 wherein the pathogenic bacteria are Clostridium perfringens or E. coli.

Assignees

• Dupont Nutrition Biosci Aps

Inventors

• Romero Luis
• Plumstead Peter
• Barnard Luke

Classifications

• A23K40/20

  by moulding, e.g. making cakes or briquettes · CPC title

• A61K38/46

  Hydrolases (3) · CPC title

• A23K50/75

  for poultry · CPC title

• A23K10/18

  of live microorganisms · CPC title

• A23K50/30

  for swines · CPC title