Cellulolytic enzyme compositions and uses thereof

Patent metadata

Field	Value
Publication number	US-11230723-B2
Application number	US-202016878267-A
Country	US
Kind code	B2
Filing date	May 19, 2020
Priority date	Aug 24, 2011
Publication date	Jan 25, 2022
Grant date	Jan 25, 2022

Abstract

The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

First claim

What is claimed is:

1. An enzyme composition comprising one or more enzymes selected from the group consisting of: (i) an Aspergillus fumigatus xylanase or homolog thereof, (ii) an Aspergillus fumigatus beta-xylosidase or homolog thereof; or (iii) a combination of (i) and (ii);
wherein the Aspergillus fumigatus xylanase or homolog thereof is selected from the group consisting of:
(i) an Aspergillus fumigatus xylanase comprising or consisting of the mature polypeptide of SEQ ID NO: 10, SEQ ID NO: 12, or SEQ ID NO: 14;
(ii) a xylanase comprising or consisting of an amino acid sequence having at least 70% sequence identity to the mature polypeptide of SEQ ID NO: 10, SEQ ID NO: 12, or SEQ ID NO: 14;
(iii) a xylanase encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 9, SEQ ID NO: 11, or SEQ ID NO: 13; and
(iv) a xylanase encoded by a polynucleotide that hybridizes under at least high stringency conditions, very high stringency conditions, with the mature polypeptide coding sequence of SEQ ID NO: 9, SEQ ID NO: 11, or SEQ ID NO: 13; or the full-length complement thereof; and
wherein the Aspergillus fumigatus beta-xylosidase or homolog thereof is selected from the group consisting of:
(i) a beta-xylosidase comprising or consisting of the mature polypeptide of SEQ ID NO: 16;
(ii) a beta-xylosidase comprising or consisting of an amino acid sequence having at least 70% sequence identity to the mature polypeptide of SEQ ID NO: 16;
(iii) a beta-xylosidase encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 15; and
(iv) a beta-xylosidase encoded by a polynucleotide that hybridizes under at least high stringency conditions, very high stringency conditions, with the mature polypeptide coding sequence of SEQ ID NO: 15 or the full-length complement thereof.

2. The enzyme composition of claim 1 , which further comprises one or more enzymes selected from the group consisting of a cellulase, a GH61 polypeptide having cellulolytic enhancing activity, a hemicellulase, an esterase, an expansin, a laccase, a ligninolytic enzyme, a pectinase, a peroxidase, a protease, and a swollenin.

3. The enzyme composition of claim 1 , wherein the xylanase comprises or consists of an amino acid sequence having at least 75% sequence identity to the mature polypeptide of SEQ ID NO: 10, SEQ ID NO: 12, or SEQ ID NO: 14.

4. The enzyme composition of claim 1 , wherein the xylanase comprises or consists of an amino acid sequence having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 10, SEQ ID NO: 12, or SEQ ID NO: 14.

5. The enzyme composition of claim 1 , wherein the xylanase comprises or consists of an amino acid sequence having at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 10, SEQ ID NO: 12, or SEQ ID NO: 14.

6. The enzyme composition of claim 1 , wherein the xylanase comprises or consists of an amino acid sequence having at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 10, SEQ ID NO: 12, or SEQ ID NO: 14.

7. The enzyme composition of claim 1 , wherein the xylanase comprises or consists of an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 10, SEQ ID NO: 12, or SEQ ID NO: 14.

8. The enzyme composition of claim 1 , wherein the xylanase comprises or consists of an amino acid sequence having at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 10, SEQ ID NO: 12, or SEQ ID NO: 14.

9. The enzyme composition of claim 1 , wherein the xylanase is encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 75% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 9, SEQ ID NO: 11, or SEQ ID NO: 13.

10. The enzyme composition of claim 1 , wherein the xylanase is encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 9, SEQ ID NO: 11, or SEQ ID NO: 13.

11. The enzyme composition of claim 1 , wherein the xylanase is encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 9, SEQ ID NO: 11, or SEQ ID NO: 13.

12. The enzyme composition of claim 1 , wherein the xylanase is encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 9, SEQ ID NO: 11, or SEQ ID NO: 13.

13. The enzyme composition of claim 1 , wherein the xylanase is encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 9, SEQ ID NO: 11, or SEQ ID NO: 13.

14. The enzyme composition of claim 1 , wherein the xylanase is encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 99% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 9, SEQ ID NO: 11, or SEQ ID NO: 13.

15. The enzyme composition of claim 1 , wherein the beta-xylosidase comprises or consists of an amino acid sequence having at least 75% sequence identity to the mature polypeptide of SEQ ID NO: 16.

16. The enzyme composition of claim 1 , wherein the beta-xylosidase comprises or consists of an amino acid sequence having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 16.

17. The enzyme composition of claim 1 , wherein the beta-xylosidase comprises or consists of an amino acid sequence having at least 85% sequence identity to the mature polypeptide of SEQ ID NO: 16.

18. The enzyme composition of claim 1 , wherein the beta-xylosidase comprises or consists of an amino acid sequence having at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 16.

19. The enzyme composition of claim 1 , wherein the beta-xylosidase comprises or consists of an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 16.

20. The enzyme composition of claim 1 , wherein the beta-xylosidase comprises or consists of an amino acid sequence having at least 99% sequence identity to the mature polypeptide of SEQ ID NO: 16.

21. The enzyme composition of claim 1 , wherein the beta-xylosidase is encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 75% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 15.

22. The enzyme composition of claim 1 , wherein the beta-xylosidase is encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 80% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 15.

23. The enzyme composition of claim 1 , wherein the beta-xylosidase is encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 85% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 15.

24. The enzyme composition of claim 1 , wherein the beta-xylosidase is encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ

Assignees

• Novozymes Inc

Inventors

• Shasky Jeffrey
• Maiyuran Suchindra
• Fischer Amanda

Classifications

• C12R2001/885

  Trichoderma · CPC title

• C12R2001/68

  Aspergillus fumigatus · CPC title

• C12P19/02

  Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title

• C12P7/10

  substrate containing cellulosic material · CPC title

• C12P19/14 Primary

  produced by the action of a carbohydrase {(EC 3.2.x)}, e.g. by alpha-amylase {, e.g. by cellulase, hemicellulase} · CPC title