Method of analyzing using analyte concentrator system having eluent generation module

Patent metadata

Field	Value
Publication number	US-11226274-B2
Application number	US-201615043332-A
Country	US
Kind code	B2
Filing date	Feb 12, 2016
Priority date	Feb 12, 2016
Publication date	Jan 18, 2022
Grant date	Jan 18, 2022

Abstract

Systems and methods for concentrating an analyte preparatory to analysis thereof include processing the effluent of an analyte concentrator to produce an eluent for eluting an analyte retained in the same or separate concentrator, and systems implementing the same. The analyte concentrator system connects the effluent outlet of an analyte concentrator column to an eluent generation module such that the substantially analyte-free effluent discharged from the analyte concentrator column passes fluidly into the eluent generation module. Eluent generated from the substantially analyte-free effluent in the eluent generation module is likewise substantially free of the analyte. The systems and methods can minimize and/or (substantially) eliminate background signal during analysis of the concentrated analyte.

First claim

What is claimed is:

1. A method of analyzing a fluid sample, comprising:
processing a fluid sample comprising an analyte in an analyte concentrator system, the analyte concentrator system comprising a multi-port valve and an analyte concentrator, the multi-port valve having a plurality of ports including a first port fluidly coupled to a port of the analyte concentrator, a second port fluidly coupled to another port of the analyte concentrator, a third port fluidly coupled to an inlet of an eluent generation module, and a fourth port fluidly coupled to an outlet of the eluent generation module, the multi-port valve having a first valve configuration and a second valve configuration, wherein processing the fluid sample comprises retaining the analyte in the analyte concentrator and discharging from the analyte concentrator an effluent of the fluid sample that is substantially free of the analyte, and wherein the multi-port valve is in the first valve configuration during processing the fluid sample to provide a first fluid flow path from the analyte concentrator to the eluent generation module and a second fluid flow path to the multi-port valve via the fourth port from the eluent generation module; the second flow path bypassing the analyte concentrator;
in the eluent generation module, generating an eluent from the discharged effluent, the generated eluent being chemically configured to elute a portion of the analyte retained in the analyte concentrator and the eluent being substantially free of the analyte, wherein the generating the eluent from the discharged effluent comprises passing the discharged effluent into the eluent generation module, the eluent generation module including an anode, a cathode, and an electrolytic chamber disposed at least partially between the anode and the cathode, the electrolytic chamber including an electrolyte reservoir, an eluent generation chamber and at last ion exchange connector, the electrolyte reservoir separated from eluent generation chamber by the ion exchange connector, wherein the passing the discharged effluent into the eluent generation module comprises passing the discharged effluent through the multi-port valve in the first valve configuration;
switching the multi-port valve from the first valve configuration to the second valve configuration to provide a third fluid flow path from the eluent generation module to the analyte concentrator;
with the multi-port valve in the second valve configuration, passing the eluent generated in the eluent generation module into the analyte concentrator system and eluting the portion of the analyte retained in the analyte concentrator with the eluent, wherein the analyte comprises at least one charged molecule or compound and the analyte concentrator comprises an ion exchange element configured to retain the at least one charged molecule or compound under a first ionic strength and to release the at least one charged molecule or compound under a second ionic strength;
passing the eluted portion of the analyte through a chromatography member to separate the eluted portion of the analyte into individual components or fractions, the chromatography member being fluidly coupled with the analyte concentrator system via the multi-port valve; and
passing the individual components or fractions from the chromatography member to an analyte detector for detecting the individual components or fractions.

2. The method of claim 1 , comprising passing the eluent in the eluent generation module into the analyte concentrator system through a fluid coupling extending between the eluent generation module and the analyte concentrator system so as to elute the portion of the analyte retained in the analyte concentrator with the eluent.

Assignees

• Dionex Corp

Inventors

• Srinivasan Kannan

Classifications

• G01N30/06

  Preparation · CPC title

• G01N30/40

  using back flushing · CPC title

• B01D15/361 Primary

  Ion-exchange · CPC title

• G01N2030/085

  using absorbing precolumn · CPC title

• G01N2030/062

  extracting sample from raw material · CPC title